Detection of in situ derivatized peptides in microbial biofilms by laser desorption 7.87 eV postionizaton mass spectrometry

A novel analytical method based on laser desorption postionization mass spectrometry (LDPI-MS) was developed to investigate the competence and sporulation factor-a pentapeptide of amino acid sequence ERGMT-within intact Bacillus subtilis biofilms. Derivatization of the neat ERGMT peptide with quinoline- and anthracene-based tags was separately used to lower the peptide ionization potential and permit direct ionization by 7.87-eV vacuum ultraviolet radiation. The techniques of mass shifting and selective ionization of the derivatized peptide were combined here to permit detection of ERGMT peptide within intact biofilms by LDPI-MS, without any prior extraction or chromatographic separation. Finally, imaging MS specific to the derivatized peptide was demonstrated on an intact biofilm using LDPI-MS. The presence of ERGMT in the biofilms was verified by bulk extraction/LC-MS. However, MALDI imaging MS analyses were unable to detect ERGMT within intact biofilms.     

Diurnal Variation in the Composition of Ileal Digesta and the Ileal Digestibilities of Nutrients in Growing Pigs

Diurnal variation in the chemical composition of digesta and its effect on nutrient digestibilities of diets varying in dietary fibre content was examined in pigs fitted with a PVTC-cannula at the terminal ileum. The pigs were fed three times a day (08:00, 16:00 and 24:00 h). A barley control diet and four barley-based diets containing two levels (100 or 200 g kg^-1 diet) of alfalfa meal or white clover meal and Cr₂O₃ as an indigestible marker were fed according to a 5×5 Latin square design. In each experimental period, digesta were collected for 1 h every third hour on each of two separate sampling days, covering in total a 24 h period. The concentration of dry matter (DM), crude protein (CP), ash and chromium (Cr) in the ileal digesta varied in relation to time of feeding. However, the patterns were similar after all three meals (08:00, 16:00 and 24:00 h) and independent of diet. The maximum DM, CP and ash contents were reached 4–5 h post-feeding. The highest (P < 0·05) DM concentration was found in the ileal digesta of pigs fed the barley control diet. The DM concentration decreased (P < 0·05) as the inclusion level of alfalfa meal and white clover meal was increased. The lowest DM concentration was found for the highest inclusion level of alfalfa meal. The ileal digestibilities of both organic matter and CP were lower 1–2 h post-feeding compared with those recorded 3–5 h later, indicating that the less digestible components of a diet have shorter transit times. The pooled ileal digestibilities of organic matter and CP from the three 8 h periods (08:00–16:00 h, 16:00–24:00 h, 24:00–08:00 h) did not differ (P < 0·05) from each other. Therefore, the estimate of digestibility can be based on digesta samples collected from any interval between two consecutive meals. However, care must be taken to obtain representative samples of digesta between meals. © 1997 SCI.     

Finding active factors from unreplicated fractional factorials utilizing the total time on test (TTT) technique

Much research has been devoted to improving the process of identifying active factors from designed experiments. Generally, the proposed methods rely on an estimate of the experimental error. Here we present a method based on the TTT (total time on test) plot, where the scaled TTT transform enables an evaluation of the contrasts independently of the experimental error. The method can be separated into two parts. The first part consists of a transformed TTT plot for a visual evaluation of data. The second part is more formal and utilizes the cumulative TTT statistic for testing the significance of contrasts. A simulation study shows the power of the method compared with competing methods. Five data sets are used to show that the conclusions drawn are consistent with those obtained using other suggested methods. Copyright © 1999 John Wiley & Sons, Ltd.     

Multivariate methods in the development of a new tablet formulation: excipient mixtures and principal properties

A tablet formulation for direct compression has previously been studied using multivariate design. An optimization study of one of the most important tablet properties, disintegration time, revealed that excipients with Principal Properties (PP's) that were predicted as suitable by the model were not represented within the studied material. The feasibility of using mixtures of excipients in the multivariate approach to tablet formulation to solve this problem has been investigated in the present study. By mixing different excipients of the same excipient class, it should be possible to obtain mixtures with the predicted PP's, which in turn should give a formulation with the desired properties. In order to investigate the utility of this approach, separate mixture designs were applied to both binders and fillers (diluents). As reported here, the Partial Least Squares Projections to Latent Structures (PLS) model developed in the previously published screening study has been validated in the sense that the interesting region of the PP space identified in it has been shown to contain excipients, pure or mixed, that give the formulation suitable properties. Formulations with suitable properties were found with the mixture experiments. The local models also offer several alternatives for the composition of the formulation that yield the desired disintegration time.     

Biogas production from grape pomace: Thermodynamic model of the process and dynamic model of the power generation system

In year 2010, the Province of San Juan – Argentina (South America) produced around 81 947 tons of grape pomace, which is a winery waste by-product. Wineries demand a great quantity of power during the three months that winemaking time lasts, which involve a high installed electric power. This power is used mainly for refrigerating the must during the fermentation. The control of temperature is crucial in winemaking as the low temperature preserves subtle fruit characters. An alternative for reducing the requirement of power from the utility electric grid is producing it locally, using the residuals generated by the own wineries. The technology for bioenergy conversion proposed to be implemented in this research for electricity generation is the anaerobic digestion of grape pomace. The aim of this research is to develop the thermodynamic equilibrium analysis of grape pomace anaerobic digestion based on the equilibrium constants for predicting the potential production of biogas and its composition. In addition, a dynamic model of a biogas-fuelled microturbine system for distributed generation applications is derived.     

Barrier properties of Al2O3 and alucone coatings and nanolaminates on flexible biopolymer films

Thin atomic layer deposited (ALD) Al₂O₃ coatings are efficient barriers against gases and vapors. Al₂O₃ coatings are, however, brittle and straining them generates defects that impair barrier properties. Flexibility of ALD-grown Al₂O₃ coatings on biopolymer substrates can be improved by separating thinner Al₂O₃ layers with inorganic-organic alucone layers. The number and size of defects were smaller for these nanolaminates compared to the thick Al₂O₃ films after straining, and hence straining deteriorated the oxygen barrier properties less when applied to the laminates than when applied to the Al₂O₃ coatings.     

A super-oscillatory lens optical microscope for subwavelength imaging

The past decade has seen an intensive effort to achieve optical imaging resolution beyond the diffraction limit. Apart from the Pendry-Veselago negative index superlens, implementation of which in optics faces challenges of losses and as yet unattainable fabrication finesse, other super-resolution approaches necessitate the lens either to be in the near proximity of the object or manufactured on it, or work only for a narrow class of samples, such as intensely luminescent or sparse objects. Here we report a new super-resolution microscope for optical imaging that beats the diffraction limit of conventional instruments and the recently demonstrated near-field optical superlens and hyperlens. This non-invasive subwavelength imaging paradigm uses a binary amplitude mask for direct focusing of laser light into a subwavelength spot in the post-evanescent field by precisely tailoring the interference of a large number of beams diffracted from a nanostructured mask. The new technology, which--in principle--has no physical limits on resolution, could be universally used for imaging at any wavelength and does not depend on the luminescence of the object, which can be tens of micrometres away from the mask. It has been implemented as a straightforward modification of a conventional microscope showing resolution better than λ/6.     

Simulation of salinity effects on past, present, and future soil organic carbon stocks

Soil organic carbon (SOC) models are used to predict changes in SOC stocks and carbon dioxide (CO(2)) emissions from soils, and have been successfully validated for non-saline soils. However, SOC models have not been developed to simulate SOC turnover in saline soils. Due to the large extent of salt-affected areas in the world, it is important to correctly predict SOC dynamics in salt-affected soils. To close this knowledge gap, we modified the Rothamsted Carbon Model (RothC) to simulate SOC turnover in salt-affected soils, using data from non-salt-affected and salt-affected soils in two agricultural regions in India (120 soils) and in Australia (160 soils). Recently we developed a decomposition rate modifier based on an incubation study of a subset of these soils. In the present study, we introduce a new method to estimate the past losses of SOC due to salinity and show how salinity affects future SOC stocks on a regional scale. Because salinity decreases decomposition rates, simulations using the decomposition rate modifier for salinity suggest an accumulation of SOC. However, if the plant inputs are also adjusted to reflect reduced plant growth under saline conditions, the simulations show a significant loss of soil carbon in the past due to salinization, with a higher average loss of SOC in Australian soils (55 t C ha(-1)) than in Indian soils (31 t C ha(-1)). There was a significant negative correlation (p < 0.05) between SOC loss and osmotic potential. Simulations of future SOC stocks with the decomposition rate modifier and the plant input modifier indicate a greater decrease in SOC in saline than in non-saline soils under future climate. The simulations of past losses of SOC due to salinity were repeated using either measured charcoal-C or the inert organic matter predicted by the Falloon et al. equation to determine how much deviation from the Falloon et al. equation affects the amount of plant inputs generated by the model for the soils used in this study. Both sets of results suggest that saline soils have lost carbon and will continue to lose carbon under future climate. This demonstrates the importance of both reduced decomposition and reduced plant input in simulations of future changes in SOC stocks in saline soils.     

A cascode modulated class-E power amplifier for wireless communications

A cascode modulated CMOS class-E power amplifier (PA) is presented in this paper. It is shown that by applying a modulated signal to the gate of the cascode transistor the output power is modulated. The main advantage of the proposed technique is a high 35 dB output power dynamic range. The peak power added efficiency (PAE) is 35%. The concept of the cascode power control of class-E RF PA operating at 2.2 GHz with 18 dBm output power was implemented in a CMOS technology and the performance has been verified by measurements. The prototype CMOS PA is tested by single tone excitation and by enhanced data rates for GSM evolution (EDGE) modulated signal. Digital predistortion is used to linearize the transfer characteristic. The EDGE spectrum mask is met and the rms error vector magnitude (EVM) is less than 4° in the entire output power range.