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排序方式: 共有1840条查询结果,搜索用时 15 毫秒
41.
Marta Louano Joana Oliveira Isabel Martins Rui Vaz Isaura Tavares 《International journal of molecular sciences》2022,23(7)
Pain transmission at the spinal cord is modulated by noradrenaline (NA)-mediated actions that arise from supraspinal areas. We studied the locus coeruleus (LC) to evaluate the expression of the cathecolamine-synthetizing enzyme tyrosine hydroxylase (TH) and search for local oxidative stress and possible consequences in descending pain modulation in a model of hydrocephalus, a disease characterized by enlargement of the cerebral ventricular system usually due to the obstruction of cerebrospinal fluid flow. Four weeks after kaolin injection into the cisterna magna, immunodetection of the catecholamine-synthetizing enzymes TH and dopamine-β-hydroxylase (DBH) was performed in the LC and spinal cord. Colocalization of the oxidative stress marker 8-OHdG (8-hydroxyguanosine; 8-OHdG), with TH in the LC was performed. Formalin was injected in the hindpaw both for behavioral nociceptive evaluation and the immunodetection of Fos expression in the spinal cord. Hydrocephalic rats presented with a higher expression of TH at the LC, of TH and DBH at the spinal dorsal horn along with decreased nociceptive behavioral responses in the second (inflammatory) phase of the formalin test, and formalin-evoked Fos expression at the spinal dorsal horn. The expression of 8-OHdG was increased in the LC neurons, with higher co-localization in TH-immunoreactive neurons. Collectively, the results indicate increased noradrenergic expression at the LC during hydrocephalus. The strong oxidative stress damage at the LC neurons may lead to local neuroprotective-mediated increases in NA levels. The increased expression of catecholamine-synthetizing enzymes along with the decreased nociception-induced neuronal activation of dorsal horn neurons and behavioral pain signs may indicate that hydrocephalus is associated with alterations in descending pain modulation. 相似文献
42.
Ângela Fernandes João C. M. Barreira Amilcar L. Antonio M. Beatriz P. P. Oliveira Anabela Martins Isabel C. F. R. Ferreira 《Food and Bioprocess Technology》2014,7(6):1606-1617
Mushrooms are very perishable foods due to their high susceptibility to moisture loss, changes in color and texture, or microbiological spoilage. Drying is considered as the most appropriate method to prevent these alterations, but it has some limitations, such as shrinkage, enzymatic and non-enzymatic browning reactions, and oxidation of lipids and vitamins. According to previous studies, irradiation might effectively attenuate the undesirable changes caused by drying process, ensuring also higher shelf-life of mushrooms and their decontamination. Electron-beam irradiation presents some technological advantages, since it allows higher dose rates and the possibility to be used in most foods/or thin products in a short period. Herein, the combined effects of electron-beam irradiation (0, 0.5, 1 and 6 kGy) and storage time (0, 6 and 12 months) were evaluated by measuring changes in nutritional parameters, namely, free sugars, tocopherols, fatty acids and antioxidant activity. As indicated by linear discriminant analysis, storage time had a higher effect on all the evaluated parameters, except fatty acids, which suffer significant changes with both factors. Overall, the obtained results indicate that electron-beam irradiation might be considered as a suitable technique, allowing long-lasting conservation periods while reducing changes induced by drying treatment. 相似文献
43.
Eighty samples of animal feeds of different origins were screened for the natural co-occurrence of cyclopiazonic acid (CPA) and aflatoxins in Portugal. Forty-five strains of Aspergillus flavus were collected from those samples and studied for their ability to produce these mycotoxins, in vitro. CPA was detected by thin-layer chromatography using Erhlich's reagent for confirmation. Aflatoxins were determined by high-pressure liquid chromatography with postcolumn iodination. Only 5 of the 80 samples (6.2%) were naturally contaminated with cyclopiazonic acid (0.16 mg/kg) and 36 (45.0%) with aflatoxin B1 (AFB1) (from 0.001 to 0.016 mg/kg). An in vitro study of the 45 strains of A. flavus was performed in cracked corn at 25 degrees C (water activity, a(w) = 0.96), incubated for 21 days to CPA production. For in vitro production of aflatoxins, the same substrate was incubated at 28 degrees C for 14 days. Nineteen of the strains (42.2%) produced CPA (ranging from 0.5 to 1.45 mg of CPA/kg) and 23 of them (51.1%) produced AFB1 (from 0.001 to 0.844 mg/kg). Only 10 isolates (22.2%) produced both CPA and AFB1 (0.05 to 0.10 mg/kg and 0.001 to 0.230 mg/kg, respectively). Thirteen strains did not produce either CPA nor AFB1. 相似文献
44.
Effect of gamma and electron beam irradiation on the physico-chemical and nutritional properties of mushrooms: a review 总被引:2,自引:0,他引:2
The short shelf-life of mushrooms is an obstacle to the distribution and marketing of the fresh product. Thus, prolonging postharvest storage, while preserving their quality, would benefit the mushroom industry as well as consumers. There has been extensive research on finding the most appropriate technology for mushrooms preservation. Gamma, electron-beam and UV irradiation have been shown to be potential tools in extending the postharvest shelf-life of fresh mushrooms. Studies evaluating the effects of ionizing radiation are available mainly in cultivated species such as Agaricus bisporus, Lentinus edodes and Pleurotus ostreatus. This review comprises a comprehensive study of the effects of irradiation on physico-chemical parameters (weight, colour, texture and pH), chemical compounds including nutrients (proteins, sugars and vitamins) and non-nutrients (phenolics, flavonoids and flavour compounds), and on biochemical parameters such as enzymatic activity of mushrooms for different species and from different regions of the world. 相似文献
45.
R. C. Martins V. V. Lopes A. A. Vicente J. A. Teixeira 《Food and Bioprocess Technology》2008,1(3):207-222
Shelf-life is defined as the time that a product is acceptable and meets the consumers expectations regarding food quality.
It is the result of the conjunction of all services in production, distribution, and consumption. Shelf-life dating is one
of the most difficult tasks in food engineering. Market pressure has lead to the implementation of shelf-life by sensory analyses,
which may not reflect the full quality spectra. Moreover, traditional methods for shelf-life dating and small-scale distribution
chain tests cannot reproduce in a laboratory the real conditions of storage, distribution, and consumption on food quality.
Today, food engineers are facing the challenges to monitor, diagnose, and control the quality and safety of food products.
The advent of nanotechnology, multivariate sensors, information systems, and complex systems will revolutionize the way we
manage, distribute, and consume foods. The informed consumer demands foods, under the legal standards, at low cost, high standards
of nutritional, sensory, and health benefits. To accommodate the new paradigms, we herein present a critical review of shelf-life
dating approaches with special emphasis in computational systems and future trends on complex systems methodologies applied
to the prediction of food quality and safety. 相似文献
46.
Silvia F. Moya Ruth L. Martins Antje Ota Edward L. Kunkes Malte Behrens Martin Schmal 《Applied Catalysis A: General》2012
The Pd on α-Al2O3 catalysts with Pd particles in the low nanometer range have been prepared by a sonochemical reduction and a colloidal method, respectively. The two catalysts differ in their particle size, the widths of their particle size distributions and the amount of carbon incorporation in the Pd lattice.The adsorptive properties of the Pd/Al2O3 samples are different as a result of the different preparation methods. The methane adsorption capacity of that sample with smaller particles is lower than that of the catalyst with larger particles and the energy of activation is nearly doubled. DRIFTS and TPD results of CO adsorption supported by transmission electron microscopy data indicate that the PdSON catalyst with smaller and more homogeneous particles than PdCOL is highly dispersed which influences the coupling-hydrogenolysis process.The catalytic activity evidenced the formation of different adspecies during methane coupling and chemisorption on both catalysts. During the hydrogenation the carbon adspecies formed mainly methane at low adsorption temperatures. The significant amount of adsorbed methane at 773 K is governed by the highly active coordination unsaturated sites at the surface. 相似文献
47.
Organic coatings based on epoxy and polyurethane matrices containing polyaniline doped with dodecyl benzene sulfonic acid (Pani-DBSA) were prepared and applied over steel plates (SAE 1020). The plates were submitted to salt spray chamber for up to 30 days in order to evaluate the corrosion protection of these coatings. The properties of the coated plates were analyzed as a function of time by electrochemical impedance spectroscopy, open circuit potential, optical microscopy, and Raman spectroscopy. In general, results indicate a decrease in the electrical resistance, increase in capacitance and decrease in open circuit potential. Epoxy based coatings have improved performance when Pani-DBSA is used as pigment, whereas for the polyurethane coatings, Pani-DBSA seems to play an adverse effect. Raman spectroscopy indicates a possible chlorination of the epoxy matrix after 30 days exposure to salt spray chamber. 相似文献
48.
49.
Jean-Pierre Martins Louis Doceul Sbastien Marol Elise Delchi Jean-Jacques Cordier Bruno Levesy Alessandro Tesini Emanuela Ciattaglia Richard Tivey Ren Gillier Christophe Abbes 《Fusion Engineering and Design》2009,84(7-11):1268-1275
In the field of the ITER port plug engineering and integration task, CEA has contributed to define proposals concerning the port plugs vacuum sealing interface with the vessel flange and the equatorial plug handling.The 2001 baseline vacuum flange sealing consisted of TIG welding of a 316L strip plate on to U shapes. This arrangement presented some issues like welding access, implementation of tools, lip consumption, complex local leak test, continuous leak checking. Therefore, an alternate sealing solution based on the use of metallic gaskets is proposed. The different technical aspects are discussed to explain how this design can simplify the maintenance and deal with safety and vacuum requirements.The design of the mechanical attachment and vacuum sealing of the plug has constantly evolved, but the associated remote handling equipment was not systematically reviewed. An update of the cask and maintenance procedure was studied in order to design it in accordance with the last generic plug flange design. This includes a concept of a gripping system that uses the plug flange bolting area and, to help the remote handling process, a cantilever assisting system is suggested to increase the reliability of the transfer operation between vacuum vessel and cask. 相似文献
50.
Abdelaaziz Merabti Diogo S. Martins Mohamed Meddi Luis S. Pereira 《Water Resources Management》2018,32(3):1087-1100
The spatial and temporal variability of droughts were studied for the Northeast Algeria using SPI and RDI computed with monthly precipitation data from 123 rainfall stations and CFSR reanalysis monthly temperature data covering the period 1979–80 to 2013–14. The gridded temperature data was interpolated to all the locations having precipitation data, thus providing to compute SPI and RDI with the time scales of 3-, 6- and 12-month with the same observed rainfall data. Spatial and temporal patterns of droughts were obtained using Principal Component Analysis in S-Mode with Varimax rotation applied to both SPI and RDI. For all time scales of both indices, two principal components were retained identifying two sub-regions that are similar and coherent for all SPI and RDI time scales. Both components explained more than 70% and 74% of drought spatial variability of SPI and RDI, respectively. The identified sub-regions are similar and coherent for all SPI and RDI time scales. The Modified Mann-Kendall test was used to assess trends of the RPC scores, which have shown non-significant trends for decreasing drought occurrence and severity in both identified drought sub-regions and all time scales. Both indices have shown a coherent and similar behavior, however with RDI likely showing to identify more severe and moderate droughts in the southern and more arid sub-region which may be due to its ability to consider influences of global warming. Results for RDI are quite uniform relative to time scales and show smaller differences among the various climates when compared with SPI. Further assessments covering the NW and NE of Algeria using longer time series should be performed to better understand the behavior of both indices. 相似文献