Phosphorylation Stoichiometries of Human Eukaryotic Initiation Factors

Eukaryotic translation initiation factors are the principal molecular effectors regulating the process converting nucleic acid to functional protein. Commonly referred to as eIFs (eukaryotic initiation factors), this suite of proteins is comprised of at least 25 individual subunits that function in a coordinated, regulated, manner during mRNA translation. Multiple facets of eIF regulation have yet to be elucidated; however, many of the necessary protein factors are phosphorylated. Herein, we have isolated, identified and quantified phosphosites from eIF2, eIF3, and eIF4G generated from log phase grown HeLa cell lysates. Our investigation is the first study to globally quantify eIF phosphosites and illustrates differences in abundance of phosphorylation between the residues of each factor. Thus, identification of those phosphosites that exhibit either high or low levels of phosphorylation under log phase growing conditions may aid researchers to concentrate their investigative efforts to specific phosphosites that potentially harbor important regulatory mechanisms germane to mRNA translation.     

地应力影响因素三维数值模拟分析

以某水电站为实例,结合数值模拟,研究了河谷下切过程中断层、错动带以及侧向剥蚀对地应力的影响情况分析,为坝址区地应力特征研究提供了科学依据。通过计算得出:在断层、断层与断层及断层与层间错动带交错部位岩体应力明显减小,而侧向剥蚀主要影响左岸应力分布,对右岸应力分布的影响不是很明显。     

Recognizing key segments of videos for video annotation by learning from web image sets

In this paper, we propose an approach of inferring the labels of unlabeled consumer videos and at the same time recognizing the key segments of the videos by learning from Web image sets for video annotation. The key segments of the videos are automatically recognized by transferring the knowledge learned from related Web image sets to the videos. We introduce an adaptive latent structural SVM method to adapt the pre-learned classifiers using Web image sets to an optimal target classifier, where the locations of the key segments are modeled as latent variables because the ground-truth of key segments are not available. We utilize a limited number of labeled videos and abundant labeled Web images for training annotation models, which significantly alleviates the time-consuming and labor-expensive collection of a large number of labeled training videos. Experiment on the two challenge datasets Columbia’s Consumer Video (CCV) and TRECVID 2014 Multimedia Event Detection (MED2014) shows our method performs better than state-of-art methods.     

Vinyl ester/clay based nanocomposites: a complementary study of vinyl ester polymerization by hyphenated rheo‐Fourier transform infrared and separate rheology/Fourier transform infrared measurements

This work is focused on the complementary information obtained from advanced in situ Fourier transform infrared (FTIR) spectroscopy and rheological measurements to correlate the structural changes with rheological properties upon polymerization of different organically modified montmorillonite clay/vinyl ester composites prepared by in situ polymerization. The microstructure and morphology of the nanocomposites were examined by X‐ray diffraction and transmission electron microscopy. The effect exerted by the presence of organic clay on the polymerization reaction of a vinyl ester based polymer matrix was evaluated. In situ and ex situ rheo‐FTIR measurements were compared to demonstrate the accuracy of this technique. © 2013 Society of Chemical Industry     

Experimental study on drying characteristics of wheat by low-field nuclear magnetic resonance

The characteristics of transverse relaxation time (T₂) of water in wheat were studied by measuring the relaxation time of low-field nuclear magnetic resonance. Analysis of the exponential distribution of T₂ revealed that wheat contains five water components. The T₂ relaxation time and distribution significantly changed during drying. The dynamic characteristics of five water components during wheat drying were determined using the signal quantity of their characteristic peaks, which showed different features. Weakly chemically bound water (T₂₂) and water ascribed to cell wall (T₂₃) were the main source of water loss. Moreover, most T₂₃ and extracellular water (T₂₄) were removed during drying. Water migration between strongly chemically bound water (T₂₁) and the other water components was bidirectional. This process was not only affected by temperature but also by wheat moisture content and proportion of the five water components. The start time of water migration advanced and growth rate of T₂₁ at the end of drying to that before drying increased at 60, 70, and 80°C. Drying at varied temperatures should be applied according to the characteristics of five water components during the drying process. In addition, high initial temperature was found to be necessary to achieve high drying rate of T₂₃, T₂₄, and free water (T₂₅). The use of drying temperature of 80°C at the early stage and then changing to 70°C reduced the heat consumption by 4.81% and increased the drying time by 9.61%.     

Effects of temperature,pH and light on the stability of aloin A and characterisation of its major degradation products

This study explored the stability and degradation products of aloin A under varying pH, temperature and light conditions usually encountered in processing. The stability of aloin A was significantly affected by temperature and pH. The content of aloin A decreased by more than 90% within 12 h at 50 °C and within 6 h at 70 °C, respectively. A significant decrease in stability was also observed at higher pHs. At pH 8.0, less than 2% of aloin A remained within 12 h. However, aloin A exhibited good stability at acidic pH levels with 94% remaining at pH 2.0 for 14 days. Light exerted no influence on the stability during the experimental period (14 days). Aloe‐emodin, elgonica‐dimers A and B were characterised as major degradation products of aloin A at pH 5.0 or below, and elgonica‐dimers were mainly formed at 4 °C as well. 10‐hydroxyaloins A and B were found under any condition except at pH 2.0 and 3.0, and they were mainly formed under high temperature, neutral‐basic and any light conditions.     

饰面清水混凝土施工工艺及质量管控的研究与应用

以上海市杨浦区某公共建筑为背景工程,结合实际工况和关键工序,从内外模板的制作、运输、连接及安装,混凝土浇筑,成品保护等方面,介绍分析了饰面清水混凝土的施工工艺流程、施工过程中的质量管控措施及相关注意事项,可为相关工程施工提供借鉴经验.     

Feature selection and hyper parameters optimization for short-term wind power forecast

Applied Intelligence - Accurate wind power forecasting plays an increasingly significant role in power grid normal operation with large-scale wind energy. The precise and stable forecasting of wind...     

Effects of isomalt on the quality of wheat flour dough and spicy wheat gluten sticks

The effects of different isomalt concentrations on the quality of wheat flour dough and spicy wheat gluten sticks (SWGS) were evaluated at the physical, structural and molecular levels. The results showed that the radial expansion rate (RER) and oil absorption rate (OAR) of SWGS increased first and then decreased with increased isomalt supplementation, which reached the maximum at 3 wt%. The pasting properties of wheat starch also changed, and the peak viscosity, breakdown and setback were decreased with the addition of isomalt. Dynamic rheological properties results showed that the storage modulus (G') and loss modulus (G'') increased with the addition of isomalt, which may be attributed to the reinforcement of gluten network structure by hydrogen bonding of isomalt. Scanning electron microscopy (SEM) images illustrated that the SWGS surface becomes smooth and the broken gluten structure was reduced after the addition of different isomalt levels compared with the control group. Overall, the wheat flour dough quality analysis showed that the addition of isomalt could generate a close binding with wheat starch and protein and further strengthen the internal structure of gluten through isomalt hydrogen bonds.     

Constitutive model and failure criterion for orthotropic ceramic matrix composites under macroscopic plane stress

To predict the nonlinear stress-strain behavior and the rupture strength of orthotropic ceramic matrix composites (CMCs) under macroscopic plane stress, a concise damage-based mechanical theory including a new constitutive model and two kinds of failure criteria was developed in the framework of continuum damage mechanics (CDM). The damage constitutive model was established using strain partitioning and damage decoupling methods. Meanwhile, the failure criteria were formulated in terms of damage energy release rate (DERR) in order to correlate the failure property of CMCs with damage driving forces, and the maximum DERR criterion and the interactive DERR criterion were suggested simultaneously. For the sake of model evaluation, the theory was applied to a typical CMC with damageable and nonlinear behavior, that is, 2D-C/SiC. The damage evolution law, strain response and rupture strength under incremental cyclic tension along both on-axis and off-axis directions were completely investigated. Comparison between theoretical predictions and experimental data illustrates that the newly developed mechanical theory is potential to give reasonable and accurate results of both stress-strain response and failure property for orthotropic CMCs.