HSV-1 and Endogenous Retroviruses as Risk Factors in Demyelination

Herpes simplex virus type 1 (HSV-1) is a neurotropic alphaherpesvirus that can infect the peripheral and central nervous systems, and it has been implicated in demyelinating and neurodegenerative processes. Transposable elements (TEs) are DNA sequences that can move from one genomic location to another. TEs have been linked to several diseases affecting the central nervous system (CNS), including multiple sclerosis (MS), a demyelinating disease of unknown etiology influenced by genetic and environmental factors. Exogenous viral transactivators may activate certain retrotransposons or class I TEs. In this context, several herpesviruses have been linked to MS, and one of them, HSV-1, might act as a risk factor by mediating processes such as molecular mimicry, remyelination, and activity of endogenous retroviruses (ERVs). Several herpesviruses have been involved in the regulation of human ERVs (HERVs), and HSV-1 in particular can modulate HERVs in cells involved in MS pathogenesis. This review exposes current knowledge about the relationship between HSV-1 and human ERVs, focusing on their contribution as a risk factor for MS.     

Identification of Proteins of Altered Abundance in Oil Palm Infected with Ganoderma boninense

Basal stem rot is a common disease that affects oil palm, causing loss of yield and finally killing the trees. The disease, caused by fungus Ganoderma boninense, devastates thousands of hectares of oil palm plantings in Southeast Asia every year. In the present study, root proteins of healthy oil palm seedlings, and those infected with G. boninense, were analyzed by 2-dimensional gel electrophoresis (2-DE). When the 2-DE profiles were analyzed for proteins, which exhibit consistent significant change of abundance upon infection with G. boninense, 21 passed our screening criteria. Subsequent analyses by mass spectrometry and database search identified caffeoyl-CoA O-methyltransferase, caffeic acid O-methyltransferase, enolase, fructokinase, cysteine synthase, malate dehydrogenase, and ATP synthase as among proteins of which abundances were markedly altered.     

Heterogeneous ion-selective membranes: the influence of the inert matrix polymer on the membrane properties

Heterogeneous ion-exchange membranes were prepared by mixing small particles of sulfonated poly(1,4-phenylene sulfide) or sulfonated styrene–divinylbenzene copolymer with a matrix polymer. Four kinds of polymers were tested as a matrix: highly flexible linear polyethylene, medium-flexible fluoroelastomer, rigid polystyrene (all highly hydrophobic) and hydrophilic cellulose prepared by hydrolysis of cellulose acetate butyrate. Membrane morphologies were studied by scanning electron microscopy, IR spectroscopy and density measurements. Subsequently, the membranes were characterised with respect to their swelling in water, electrochemical characteristics and transport properties. Ion-exchange capacity and proton conductivity together with the permeability to hydrogen and methanol were investigated. The important impact of the ion-exchange particles as well as of the polymer matrix used was observed. The increasing rigidity of the polymer matrix resulted in a decrease in membrane permeability, but at the same time in deterioration of its ion-exchange capacity and subsequently of the proton conductivity, too. This was explained in terms of the limited elasticity of the polymer matrix, in each sample under study, which does not allow the ion-exchange particles to swell to an identical degree.     

Family Functioning and Children's Adjustment: Associations Among Parents' Depressed Mood, Marital Hostility, Parent-Child Hostility, and Children's Adjustment.

Relations between parents' depressed mood, marital conflict, parent-child hostility, and children's adjustment were examined in a community sample of 136 ten-year-olds and their parents. Videotaped observational and self-report data were used to examine these relations in path analyses. A proposed model was tested in which mothers' and fathers' depressed mood and marital hostility were associated with children's adjustment problems through disruptions in parent-child relationships. Results showed that both mothers' and fathers' marital hostility were linked to parent-child hostility, which in turn was linked to children's internalizing problems. Fathers' depressed mood was linked to children's internalizing problems indirectly through father-child hostility. Fathers' depressed mood was directly linked to children's externalizing problems and indirectly linked through father-child hostility. For mothers, marital hostility was directly linked to children's externalizing problems, and marital hostility in fathers was indirectly linked to children's externalizing problems through father-child hostility. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Intermittent hemodialysis treatment in cefepime‐induced neurotoxicity: Case report,pharmacokinetic modeling,and review of the literature

Cefepime is a broad‐spectrum cephalosporin indicated for in‐hospital treatment of severe infections. Acute neurotoxicity, an increasingly recognized adverse effect of this drug in an overdose, predominantly affects patients with reduced renal function. Although dialytic approaches have been advocated to treat this condition, their role in this indication remains unclear. We report the case of an 88‐year‐old female patient with impaired renal function who developed life‐threatening neurologic symptoms during cefepime therapy. She was treated with two intermittent 3‐hour high‐flux, high‐efficiency hemodialysis sessions. Serial pre‐, post‐, and peridialytic (pre‐ and postfilter) serum cefepime concentrations were measured. Pharmacokinetic modeling showed that this dialytic strategy allowed for serum cefepime concentrations to return to the estimated nontoxic range 15 hours earlier than would have been the case without an intervention. The patient made a full clinical recovery over the next 48 hours. We conclude that at least 1 session of intermittent hemodialysis may shorten the time to return to the nontoxic range in severe clinically patent intoxication. It should be considered early in its clinical course pending chemical confirmation, even in frail elderly patients. Careful dosage adjustment and a high index of suspicion are essential in this population.     

Physical properties of muffins sweetened with steviol glycosides as the sucrose replacement

Overconsumption of sweets contributes to increasing obesity among children and adults. Functional food brings an opportunity to improve nutritional value of commonly eaten products, e.g., pastry products. New trends in the food industry tend to produce low-calorie products by replacing sugar or fat with additives having pro-health benefits. The aim of this study was to determine the effect of replacing sucrose with steviol glycosides (natural non-caloric sweetener) on the quality properties of bakery products. The analysis of muffin’s texture, color, cooking yield, browning index, and sensory analysis were performed. The study showed that a 25% addition of steviol sweetener (as a sucrose replacement) was the most appropriate modification of the basic formula. The resulting muffins gained sensory attractiveness and health-promoting qualities. Moreover, the study showed that a reduction of sucrose in excess of 50% had a negative impact on the quality of muffins and their sensory profiles.     

Modeling of Three-Phase Fibrous Composite Using the Asymptotic Homogenization Method

A three-phase concentric fiber-reinforced periodic composite is considered wherein the constituents exhibit piezoelectric properties. The cross-section of the periodic cell is a regular hexagon with two concentric circles and the periodicity is the same in two directions at an angle ~ /3. Simple closed-form expressions are obtained for the effective properties of this composite by means of the asymptotic homogenization method. Numerical computations have been done. The analytical solution of the required resulting plane- and antiplane-strain local problems, which turns out to be only two, makes use of potential methods of a complex variable and properties of Weierstrass elliptic and related functions of periods (1,0) and (cos ~ /3, sin ~ /3). Benveniste's universal type of relations for this composite are satisfied. Comparison with other models is shown.     

Anodes for glucose fuel cells based on carbonized nanofibers with embedded carbon nanotubes

Electrodes made of carbonized polyacrylonitryle (cPAN) nanofibers, with and without embedded multiwall carbon nanotubes (MWCNTs) were fabricated by the electrospinning (ES) process and evaluated as anodes in glucose fuel cell (FC) application. The effect of several processing and structural characteristics, such as the presence of MWCNTs, polymer concentration in the ES solution and silver electroless plating on FC performance were measured. The carbon electrodes were successful as anodes showing significant activity even without additional silver catalyst, with noticeable improvement by the incorporation of MWCNTs. The orientation of graphitic layers along the fiber axis and the coherence of layer packing were shown to be important for enhanced electrode activity. The maximal values of open circuit voltage (OCV) and peak of power density (PP_D) of unmetalized electrodes, 0.4 V and 30 μW/cm² respectively, were found to be for composite cPAN/CNT electrode. Electroless silver metallization of the carbon nanofiber electrodes leads to much better FC performance. Maximal values of OCV and PP_D of silvered carbon electrodes were measured to be about 0.9 V and 400 μW/cm², respectively. Thus, carbonized nanofibers with embedded MWCNTs may form a good basis for glucose FC anodes, but better metallization and cell-configuration allowing proper mixing are required.     

Factors affecting the mechanical and viscoelastic properties of acrylic bone cement

The aim of this paper is to report a series of experiments investigating the factors that influence the viscoelastic properties of acrylic bone cement. The effects of the brand of cement, the length of time since mixing, temperature, the hydration of the cement, and the influence of fat and or blood in the environment on the creep and stress relaxation behavior of the cement have been studied in laboratory-prepared specimens in tension, compression and four point bending. Although there are significant differences in the viscoelastic behavior of some of the different brands of polymethylmethacrylate based cements, these differences are small by comparison with the major effects that can be exerted by the length of time since mixing and some environmental factors. These effects have important practical consequences, especially with regard to the ability of bench top and theoretical studies to predict reliably the mechanical and viscoelastic behavior of acrylic cement in vivo.     

Using Bounded Model Checking for Coverage Analysis of Safety-Critical Software in an Industrial Setting

Testing and Bounded Model Checking (BMC) are two techniques used in Software Verification for bug-hunting. They are expression of two different philosophies: testing is used on the compiled code and it is more suited to find errors in common behaviors, while BMC is used on the source code to find errors in uncommon behaviors of the system. Nowadays, testing is by far the most used technique for software verification in industry: it is easy to use and even when no error is found, it can release a set of tests certifying the (partial) correctness of the compiled system. In the case of safety critical software, in order to increase the confidence of the correctness of the compiled system, it is often required that the provided set of tests covers 100% of the code. This requirement, however, substantially increases the costs associated to the testing phase, since it often involves the manual generation of tests. In this paper we show how BMC can be productively applied to the Software Verification process in industry. In particular, we show how to productively use a Bounded Model Checker for C programs (CBMC) as an automatic test generator for the Coverage Analysis of Safety Critical Software. In particular, we experimented CBMC on a subset of the modules of the European Train Control System (ETCS) of the European Rail Traffic Management System (ERTMS) source code, an industrial system for the control of the traffic railway, provided by Ansaldo STS. The Code of the ERTMS/ETCS, with thousands of lines, has been used as trial application with CBMC obtaining a set of tests satisfying the target 100% code coverage, requested by the CENELEC EN50128 guidelines for software development of safety critical systems. The use of CBMC for test generation led to a dramatic increase in the productivity of the entire Software Development process by substantially reducing the costs of the testing phase. To the best of our knowledge, this is the first time that BMC techniques have been used in an industrial setting for automatically generating tests achieving full coverage of Safety-Critical Software. The positive results demonstrate the maturity of Bounded Model Checking techniques for automatic test generation in industry.