碳酸盐岩储层多级注入闭合酸压技术研究与应用

本文阐述了针对陕甘宁盆地中部气田碳酸盐岩低渗储层的特点,采用系统工程方法开展的多级注入闭合酸压技术研究,介绍了利用研制的多级注入、二维及拟三维酸压设计软件模拟研究所获得的一些重要认识;提出了该项技术的现场设计原则及效果评估方法,介绍了现场应用效果。文中强调指出,对低渗及特低渗碳酸盐岩气藏,多级注入闭合酸压技术是最好的增产措施。     

服务计算中避免死锁和活锁的资源分配算法

服务计算系统资源层的目标是满足服务实例的资源分配需求,保证其成功执行而不陷入死锁和活锁.首先将服务资源分配行为形式化为有限状态机;其次提出一种避免死锁和活锁的资源分配算法,它采用并发请求资源的方式,且不需要并发服务实例之间交换消息.仿真实验结果表明,该算法能够避免服务资源分配过程中的死锁和活锁,表现出较高资源分配性能.     

燃气活塞式弹翼展开动力系统内弹道性能分析

为了给某型燃气活塞式弹翼展开动力系统的研制和改进提供理论依据,根据弹翼展开机构的具体结构,考虑展开动力系统与展开执行机构之间的耦合联动关系及弹翼展开过程中的气动负载等,建立了该动力系统的内弹道性能分析模型并完成了性能计算,将计算结果与试验数据进行了对比分析,两者基本吻合.研究结果表明,该模型可为工程实践提供理论支持.     

动态特征选择算法对恶意行为检测的优化研究

针对互联网中存在的恶意行为,特别是社交网络应用中的在线恶意行为,通常使用基于用户多维特征的聚类分析算法进行检测。提出一种动态特征选择算法(DFSA),使用具有特征加权熵的模糊C均值目标函数,首先为参数构建一个学习模式,自动计算每个特征权重,并剔除权重小于阈值的特征,动态选择重要的特征,迭代地更新隶属函数、簇中心和特征权重直到最优化为止,最后识别出具有高精度的恶意用户行为簇。仿真结果表明,对比SDAFS算法、ELAFC算法和NADMB算法,DFSA算法在Rand指数、Jaccard指数和归一化互信息量3个主要性能指标上均有改善。     

Having a Same Type IIS Enzyme’s Restriction Site on Guide RNA Sequence Does Not Affect Golden Gate (GG) Cloning and Subsequent CRISPR/Cas Mutagenesis

Golden gate/modular cloning facilitates faster and more efficient cloning by utilizing the unique features of the type IIS restriction enzymes. However, it is known that targeted insertion of DNA fragment(s) must not include internal type IIS restriction recognition sites. In the case of cloning CRISPR constructs by using golden gate (GG) cloning, this narrows down the scope of guide RNA (gRNA) picks because the selection of a good gRNA for successful genome editing requires some obligation of fulfillment, and it is unwanted if a good gRNA candidate cannot be picked only because it has an internal type IIS restriction recognition site. In this article, we have shown that the presence of a type IIS restriction recognition site in a gRNA does not affect cloning and subsequent genome editing. After each step of GG reactions, correct insertions of gRNAs were verified by colony color and restriction digestion and were further confirmed by sequencing. Finally, the final vector containing a Cas12a nuclease and four gRNAs was used for Agrobacterium-mediated citrus cell transformation. Sequencing of PCR amplicons flanking gRNA-2 showed a substitution (C to T) mutation in transgenic plants. The knowledge derived from this study could widen the scope of GG cloning, particularly of gRNAs selection for GG-mediated cloning into CRISPR vectors.     

大型公路桥梁施工监理探讨

根据广深高速公路等施工实践经验,对公路桥梁水下混凝土灌注桩、主体钢筋混凝土、后张法预应力箱梁制作、桥梁架设的施工与监理过程中控制关键点进行探讨,以期加强施工与监理的工作质量。     

丁羟推进剂(AP/A1/wax)爆轰参数的理论计算

丁羟推进剂在特定场合具有炸药行为,采用B-W法则预测丁羟推进剂的爆炸产物,同时运用盖斯定律、热力学定律及C-J爆轰理论对爆轰参数进行理论计算.结果表明:理论计算得出的爆轰参数与实验测得的结果相当,说明该计算方法运用恰当,对丁羟推进剂发生爆轰事故评估以及改制其它含能材料具有一定的指导意义.     

水下武器探测和信息处理的综合化与平台多样化

近年来,鱼雷技术内涵的延伸和功能的扩展给水下武器探测和信息处理带来了新的应用和发展空间,水下探测与信息处理模式呈现出了多样化和综合化的新局面,主要体现在新的探测平台带来的优势。未来作战模式的改变,使得实现平台—武器探测一体化以及武器探测与信息处理技术的融合成为其技术发展的主流趋势。利用平台的成套传感器,把接敌信息下载到武器上,以改进其捕获目标能力和提高其自导性能;利用平台处理器来增强武器的处理能力;利用平台显示器可以实时显示武器的战术态势;利用平台能实现武器对威胁的快速响应等等。当前常规鱼雷、低速UUV和反鱼雷鱼雷的技术发展分别代表了水下武器探测技术的3个主要应用方向。本文试图跟踪近年来这3种武器平台的发展状况以及与其相关的探测技术的发展趋势,归纳了这3种武器平台探测与信息处理技术的发展动向,并阐述了其目前的研究状况和面临的主要问题。     

A Predictive Instrument for Sensitive and Expedited Measurement of Ultra-Barrier Permeation

The reliable operation of flexible display devices poses a significant engineering challenge regarding the metrology of high barriers against water vapor. No reliable results have been reported in the range of 10^–6 g∙(m²∙d)⁻¹, and there is no standard ultra-barrier for calibration. To detect trace amount of water vapor permeation through an ultra-barrier with extremely high sensitivity and a greatly reduced test period, a predictive instrument was developed by integrating permeation models into high-sensitivity mass spectrometry measurement based on dynamic accumulation, detection, and evacuation of the permeant. Detection reliability was ensured by means of calibration using a standard polymer sample. After calibration, the lower detection limit for water vapor permeation is in the range of 10^–7 g∙(m²∙d)⁻¹, which satisfies the ultra-barrier requirement. Predictive permeation models were developed and evaluated using experimental data so that the steady-state permeation rate can be forecasted from non-steady-state results, thus enabling effective measurement of ultra-barrier permeation within a significantly shorter test period.     

Application of laser-induced breakdown spectroscopy for detecting leakage of boric acid

Recently, many studies have been conducted on developing on-line leak detection techniques in the reactor vessel head of nuclear power plants. One of them, laser-induced breakdown spectroscopy (LIBS), an effective technique of leak detection, which is a kind of atomic emission spectroscopy that uses a highly energetic laser pulse as the excitation source, has been of interest due to the fast and reliable identification of chemical elements for the precipitates formed by the leakage of cooling water containing boric acid. Experimental setup and tests were performed for the boric acid precipitates formed on the steel for various conditions with a Q-switched 532 nm Nd:YAG laser, optical lenses, an Echelle spectrograph and an intensified charge-coupled device detector. The LIBS system could be applied to obtain a spectral line (～249. 2 nm) corresponding to the atomic boron emission line for the precipitates of boric acid formed on low alloy steels, SA508 and SA533, as a substrate. The LIBS technique based on characterizing boric acid deposits might be used for detecting the leakage of cooling water in reactor vessel head of nuclear power plants.