Effects of Clinorotation on Growth and Chlorophyll Content of Rice Seeds

Microgravity, as a different environment, has been shown to affect plant growth and development (Sievers et al. 1996; Sack 1997). In the present study, effects of slow clinorotation (2 rpm) on growth and chlorophyll content in rice (variety: PRH-10) seedlings were investigated. Rice seeds were clinorotated continuously for 3, 5 and 7 days under ambient conditions. Root and shoot lengths and weights of rice seedlings were measured on the third, fifth and seventh day. Chlorophyll was extracted using N, N-Dimethylformamide (DMF). Absorption and fluorescence spectra of chlorophyll were recorded. Chlorophyll a, chlorophyll b and total chlorophyll contents were calculated from absorption spectra using Arnon’s method. Results showed an increase in root and shoot lengths in clinorotated samples. Similar results were obtained for root and shoot weights. Absorption spectra of chlorophyll showed no shift in the absorption peaks. Chlorophyll content was increased in clinorotated samples as compared to the controls. Interestingly, the difference between chlorophyll content in control and clinorotated samples decreased as the number of days of clinorotation increased. Chlorophyll a/b ratio was lowered in clinorotated samples as compared to the controls. These results suggest that slow clinorotation (2 rpm) affects plant growth and chlorophyll content in rice seedlings.     

Model validation under epistemic uncertainty

This paper develops a methodology to assess the validity of computational models when some quantities may be affected by epistemic uncertainty. Three types of epistemic uncertainty regarding input random variables - interval data, sparse point data, and probability distributions with parameter uncertainty - are considered. When the model inputs are described using sparse point data and/or interval data, a likelihood-based methodology is used to represent these variables as probability distributions. Two approaches - a parametric approach and a non-parametric approach - are pursued for this purpose. While the parametric approach leads to a family of distributions due to distribution parameter uncertainty, the principles of conditional probability and total probability can be used to integrate the family of distributions into a single distribution. The non-parametric approach directly yields a single probability distribution. The probabilistic model predictions are compared against experimental observations, which may again be point data or interval data. A generalized likelihood function is constructed for Bayesian updating, and the posterior distribution of the model output is estimated. The Bayes factor metric is extended to assess the validity of the model under both aleatory and epistemic uncertainty and to estimate the confidence in the model prediction. The proposed method is illustrated using a numerical example.     

Curvelet-based fingerprint anti-spoofing

Existing perspiration-based liveness detection algorithms need two successive images (captured in certain time interval), hence they are slow and not useful for real-time applications. Liveness detection methods using extra hardware increase the cost of the system. To alleviate these problems, we propose new curvelet-based method which needs only one fingerprint to detect liveness. Wavelets are very effective in representing objects with isolated point singularities, but fail to represent line and curve singularities. Curvelet transform allows representing singularities along curves in a more efficient way than the wavelets. Ridges oriented in different directions in a fingerprint image are curved; hence curvelets are very significant to characterize fingerprint texture. Textural measures based on curvelet energy and co-occurrence signatures are used to characterize fingerprint image. Dimensionalities of feature sets are reduced by running Pudil’s sequential forward floating selection (SFFS) algorithm. Curvelet energy and co-occurrence signatures are independently tested on three different classifiers: AdaBoost.M1, support vector machine and alternating decision tree. Finally, all the aforementioned classifiers are fused using the “Majority Voting Rule” to form an ensemble classifier. A fingerprint database consisting of 185 real, 90 Fun-Doh and 150 Gummy fingerprints is created by using varieties of artificial materials for casts and moulds of spoof fingerprints. Performance of the new liveness detection approach is found very promising, as it needs only one fingerprint and no extra hardware to detect vitality.     

Experimental Comparison of the Effects of Nanometric and Micrometric Particulates on the Tensile Properties and Fracture Behavior of Al Composites at Room and Elevated Temperatures

This article studies the influence of nanometric (n-SiC_p) and micrometric-scale SiC particulates (μ-SiC_p) on the tensile properties of the Al 7075 alloy. The unreinforced Al and its composites were synthesized using the powder metallurgy (P/M) route and were tested uniaxially in tension at both room and elevated temperatures. Aging behavior was studied to observe any effect of the reinforcement on the aging kinetics and hardness of the composites. X-ray diffraction was performed to determine the crystal structures of the raw materials and any reaction phase formed in the composites. The n-SiC_p were not dispersed uniformly in the Al matrix and clustered mainly at the grain boundaries. The stiffness of the composites increased and the ductility decreased with an increase in the volume fraction of the n-SiC_p. The n-SiC_p proved to be a better reinforcement than the traditional μ-SiC_p in terms of imparting higher ductility to the composite. Fractography and microscopy using optical, scanning electron, and transmission electron microscopes were performed for failure and microstructural analysis of all the materials. At room temperature, the fracture altered from ductile in the unreinforced Al to brittle in the composites. At an elevated temperature, the fracture mechanism transformed from brittle to ductile rupture in the composites.     

Practices,projects and portfolios: Current research trends and new directions

Project portfolio management (PPM) bridges strategy and project management. Traditional research in PPM has primarily investigated the rational, top-down and structural aspects of strategizing. By doing so, it has failed to focus on the underlying practices that are triggered by the strategy and how these practices frame strategy implementation. Practice-based research provides a methodological lens to explore the reality of strategic enactment through the project portfolio. Practice-based perspectives are under-represented in PPM research; therefore the aim of this paper is to provide an agenda for further practice-based research in PPM. Central to this agenda is a concern with various aspects of practice, including its discursivity, representation, dynamic capabilities, leadership and materiality.     

Effect of Radial Clearance on Wear and Contact Pressure of Hard-on-Hard Hip Prostheses Using Finite Element Concepts

Wear of the bearing surface increases the revision rate of artificial hip replacements and is influenced by the radial clearances between the acetabulum cup and the femoral head. The objective of this article is to determine the effect of various radial clearance values over the contact pressure and wear of the hard-on-hard—that is, polycrystalline diamond (PCD)—hip prostheses using finite element concepts for normal walking conditions. The wear of the hip bearing surface is determined by considering the contact pressures obtained from the hip gait instants of normal walking activity and sliding distance determined from the three-dimensional hip gait motions. The radial clearance value of 0.05 mm showed less volumetric wear rate when compared with other radial clearance values. Overall, it is recommended that the low radial clearance between the contacting pair is suitable for PCD-on-PCD hip prostheses.     

Effect of microstructure on micromechanical performance of dry cortical bone tissues

The mechanical properties of bone depend on composition and structure. Previous studies have focused on macroscopic fracture behavior of bone. In the present study, we performed microindentation studies to understand the deformation properties and microcrack–microstructure interactions of dry cortical bone. Dry cortical bone tissues from lamb femurs were tested using Vickers indentation with loads of 0.245–9.8 N. We examined the effect of bone microstructure on deformation and crack propagation using scanning electron microscopy (SEM). The results showed the significant effect of cortical bone microstructure on indentation deformation and microcrack propagation. The indentation deformation of the dry cortical bone was basically plastic at any applied load with a pronounced viscoelastic recovery, in particular at lower loads. More microcracks up to a length of approximately 20 μm occurred when the applied load was increased. At loads of 4.9 N and higher, most microcracks were found to develop from the boundaries of haversian canals, osteocyte lacunae and canaliculi. Some microcracks propagated from the parallel direction of the longitudinal interstitial lamellae. At loads 0.45 N and lower, no visible microcracks were observed.     

Nitrogen redistribution, microstructure, and elastic constant evaluation using ultrasonics in aged 316LN stainless steels

The presence of even dilute concentrations of nitrogen (0.08 mass pct) is found to have a strong influence on the microstructure of a nuclear grade 316LN austenitic stainless steel, thermally aged at 1123 K. High-resolution transmission electron microscopy (TEM) evidence of pre-precipitation reactions involving formation of Cr-N rich clusters prior to Cr₂N precipitation has been presented. A tendency for cellular precipitation has been observed on continued aging above 500 hours. Beyond 1000 hours, chi precipitates are the most frequently seen phase. The observed microstructural variations correlate well with ultrasonic velocity and attenuation measurements. The present study reveals that nitrogen in solid solution decreases all the elastic constants, namely longitudinal modulus, elastic modulus, shear modulus, and bulk modulus in this steel but has a negligible effect on Poisson’s ratio. The stages associated with the precipitation of intragranular coherent Cr₂N are, however, associated with an increase in all the elastic constants including Poisson’s ratio.     

Particle tracking techniques for electrokinetic microchannel flows

We have applied particle tracking techniques to obtain spatially resolved velocity measurements in electrokinetic flow devices. Both micrometer-resolution particle image velocimetry (micro-PMV) and particle tracking velocimetry (PTV) techniques have been used to quantify and study flow phenomena in electrokinetic systems applicable to microfluidic bioanalytical devices. To make the flow measurements quantitative, we performed a series of seed particle calibration experiments. First, we measure the electroosmotic wall mobility of a borosilicate rectangular capillary (40 by 400 microm) using current monitoring. In addition to this wall mobility characterization, we apply PTV to determine the electrophoretic mobilities of more than 1,000 fluorescent microsphere particles in aqueous buffer solutions. Particles from this calibrated particle/ buffer mixture are then introduced into two electrokinetic flow systems for particle tracking flow experiments. In these experiments, we use micro-PIV, together with an electric field prediction, to obtain electroosmotic flow bulk fluid velocity measurements. The first example flow system is a microchannel intersection where we demonstrate a detailed documentation of the similitude between the electrical fields and the velocity fields in an electrokinetic system with uniform zeta potential, zeta. In the second system, we apply micro-PIV to a microchannel system with nonuniform zeta. The latter experiment provides a simultaneous measurement of two distinct wall mobilities within the microchannel.     

A middleware‐transparent approach to developing distributed applications

An innovative middleware‐transparent approach to developing distributed applications is presented. The approach uses an aspect‐oriented software development technique to separate an application's middleware‐independent functionality from its middleware‐specific functionality. Application elements that are specific to the middleware are localized in aspects that can be seamlessly integrated into middleware‐independent application designs. The middleware‐transparent approach is used to decouple business functionality from middleware‐specific functionality. The decoupling allows developers to change middleware application elements without significantly modifying business functionality. Middleware technologies such as Java Remote Method Invocation (RMI), Jini, Simple Object Access Protocol (SOAP) remote procedure call (RPC) and .Net are used as examples to illustrate the approach. Copyright © 2005 John Wiley & Sons, Ltd.