Preparation and characterization of elastin-like polypeptide scaffolds for local delivery of antibiotics and proteins

Tissue engineering applications could benefit from simultaneous release of growth factors, signaling molecules, and antibiotics to obtain optimal healing of tissues. Elastin-like polypeptides (ELPs) are genetically engineered polymers that possess good biocompatibility, are biodegradable, and exhibit mechanical properties similar to natural elastin. In addition, ELPs exhibit a characteristic inverse phase transition temperature (T_t). This T_t behavior is widely exploited in hyperthermia mediated drug delivery. The objectives of this research were to prepare ELP hydrogel scaffolds using a novel ultrasonication method and to investigate the release of a model protein (bovine serum albumin, BSA) and a commonly used antibiotic in periodontal therapy (doxycycline) from the scaffolds at two different temperatures (25?°C <T_t vs. 37?°C >T_t). Both BSA and doxycycline showed a gradual time dependent release and showed a trend of higher release fractions with higher loading doses. Based on the comparison between the release profiles at the two selected temperatures, the release was higher at 37?°C compared to that at 25?°C for both the loading concentrations of doxycycline (0.05 and 0.1?% v/v) and only one of the loading concentrations of BSA (0.5?% v/v) studied, while the release was higher at 25?°C compared to that at 37?°C only for the other loading concentration of BSA (1?% v/v) studied. These results suggested that the drug molecular weight and loading concentration were significant factors that affected the release kinetics. The experiments in this study demonstrated that the ELP hydrogel scaffolds can successfully release proteins and antibiotics critical to tissue engineering.     

Isobutrin from butea monosperma (flame of the forest): a promising new natural sensitizer belonging to chalcone class

In this work, "isobutrin", an ecofriendly sensitizer that is extracted from Butea monosperma (commonly known as "Flame of the Forest") flowers, is introduced. It is a bright yellow pigment belonging to the chalcone class and is examined as a sensitizer for optoelectronic applications. It is observed that chelation of this dye with Ti ions results into a strong dye-TiO(2) charge transfer (DTCT) band in the visible region. This Ti-Isobutrin chelate is stable, irreversible and its formation is studied using Benesi-Hildebrand plot. The locations of HOMO-LUMO states of the Ti-isobutrin chelate and the corresponding band alignment with TiO(2) are obtained. Also, a thermal stability test revealed that isobutrin is stable above 100 °C.     

Macrocyclization of a Ligand Targeting a Toxic RNA Dramatically Improves Potency

RNA molecules both contribute to and are causative of many human diseases. One method to perturb RNA function is to target its structure with small molecules. However, discovering bioactive ligands for RNA targets is challenging. Here, we show that the bioactivity of a linear dimeric ligand that inactivates the RNA trinucleotide repeat expansion that causes myotonic dystrophy type 1 [DM1; r(CUG)^exp] can be improved by macrocyclization. Indeed, the macrocyclic compound is ten times more potent than the linear compound for improving DM1-associated defects in cells, including in patient-derived myotubes (muscle cells). This enhancement in potency is due to the macrocycle's increased affinity and selectively for the target, which inhibit r(CUG)^exp’s toxic interaction with muscleblind-like 1 (MBNL1), and its superior cell permeability. Macrocyclization could prove to be an effective way to enhance the bioactivity of modularly assembled ligands targeting RNA.     

Photophysical studies of polyvinylcarbazole polymer films

Polyvinylcarbazole (PVK) films have been prepared by solution method. Their absorption spectra, photoluminescence, and electroluminescence have been studied. Absorption in pure PVK is found to be high at lower wavelengths in UV region and decreases in visible range. In case of I₂ doped PVK, absorption is observed in visible region also. The photoluminescence is obtained in pure PVK when exited by violet or blue light but in case of I₂ doped PVK, similar excitation gives low intensity peaks in yellow–green region. The results indicate that I₂ levels in PVK causes absorption in 400–600 nm wavelength range and makes the deexcitation of the molecules nonradiative. Light emission by application of ac voltage from mains up to 100 V has been studied. Nonlinear increase in emission intensity has been observed with increasing voltage. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 104: 722–726, 2007     

Influence of ion-beam sputtering deposition parameters on highly photosensitive and transparent CdZnO thin films

CdZnO thin films with a nominal thickness of ~200 nm were grown on c-plane sapphire substrates by dual ion-beam sputtering deposition technique. The effect of substrate temperature (300–600 °C) and gas ambience on structural, morphological, compositional and opto-electronic properties was studied. X-ray diffraction patterns confirmed that all the films were polycrystalline in nature and were preferentially oriented along the c-axis. It was revealed that the films grown at Ar/O₂ ratio of 4:1 were structurally more ordered and the film quality was found to be the best at 500 °C. The compositional studies specify that approximately 11.8 at.% of cadmium were present in the film deposited at 300 °C in Ar–O₂ mixture. Investigations on optical properties by photoluminescence and absorption studies indicate band gap shrinkage with the increase in argon partial pressure and substrate temperature. It was found that photosensitivity of the deposited films was highly dependent on growth conditions. The photosensitivity was found to be 5000-fold higher for CdZnO film grown at 600 °C in Ar–O₂ ambience compared to the best reported result, and this was promising to realize high-performance opto-electronic devices on such CdZnO films.     

Short Antimicrobial Lipo‐α/γ‐AA Hybrid Peptides

The last two decades have seen the rise of antimicrobial peptides (AMPs) to combat emerging antibiotic resistance. Herein we report the solid‐phase synthesis of short lipidated α/γ‐AA hybrid peptides. This family of lipo‐chimeric peptidomimetics displays potent and broad‐spectrum antimicrobial activity against a range of multi‐drug resistant Gram‐positive and Gram‐negative bacteria. These lipo‐α/γ‐AA hybrid peptides also demonstrate high biological specificity, with no hemolytic activity towards red blood cells. Fluorescence microscopy suggests that these lipo‐α/γ‐AA chimeric peptides can mimic the mode of action of AMPs and kill bacterial pathogens via membrane disintegration. As the composition of these chimeric peptides is simple, therapeutic development could be economically feasible and amenable for a variety of antimicrobial applications.     

Structural characteristics,fluorescence quenching,and antioxidant activity of the arabinogalactan protein-rich fraction from senna (<Emphasis Type="Italic">Cassia angustifolia</Emphasis>) leaves

Senna (Cassia angustifolia) is widely used in Indian folk medicine for the prevention of various disorders. Herein, we have demonstrated that water extracted arabinogalactan protein (AGP) rich fraction possesses strong antioxidative activity. The AGP rich fraction was analyzed using chemical, chromatographic, and spectroscopic methods. Effect of water extracted polymers on bovine serum albumin spectrum was determined using excitation wavelength of 282 nm. The antioxidant capacity of this fraction was studied by ferric reducing antioxidant power (FRAP) and DPPH radical assays. This polymeric fraction, ≥88% of which gets precipitated with Yariv reagent, consisted mainly of (1→5)-/(1→3,5)-linked α-arabinosyl, (1→3)-/(1→3,6)-linked-galactosyl residues and terminal arabinofuranosyl residues. Its in vitro antioxidant capacity is comparable to that of standard antioxidants. Fluorescence quenching studies furnishes evidence for the interaction of the arabinogalactan protein with bovine serum albumin.     

Mechano‐Morphological Characterization of Polyethylene‐Glycol Based Polyurethane Microgel

Segmental polyurethanes (PU) with hydrophilic segments form colloidal dispersions which are ultimately arrested into gel‐like structure in aqueous continuous phase owing to the differential interactions between polymer and solvent. These structural states of amphiphilic PUs evolve hierarchically, but the structure‐function correlation between PU colloidal dispersion and gels is not clear. Here, this correlation is defined from the mechanomorphology of hydrophilic polyethylene glycol based PU which forms dispersions and finally transforms into gel‐structure. Morphological and rheological analyses show that PU with comparable hydrophilic and hydrophobic content forms attractive colloids with self‐similar fractal microstructures whereas PU with increased hydrophilic character forms space‐filling colloids without any defined organization. Furthermore, colloidal dispersions are densified under shear or gravity to form gel where gel mechanics is defined by colloidal particle organization and the morphology is dependent on gelation mode. This stepwise organization of PU colloidal particles into microgel can independently control microgel mechanics and morphology.

     

A study on vulnerability and presentation attack detection in palmprint verification system

As biometric systems become ubiquitous in the domain of personal authentication, it is of utmost importance that these systems are secured against attacks. Among various types of attacks on biometric systems, the presentation attack, which involves presenting a fake copy (artefact) of the real biometric to the biometric sensor to gain illegitimate access, is the most common one. Despite the serious threat posed by these attacks, not much work has been done to address this vulnerability in palmprint-based biometric systems. This paper demonstrates the vulnerability of a palmprint verification system to presentation attacks and proposes a novel presentation attack detection (PAD) approach to discriminating between real biometric samples and artefacts. The proposed PAD approach is inspired by a work that established relationship between the surface reflectance and a set of statistical features extracted from the image. Specifically, statistical features computed from the distributions of pixel intensities, sub-band wavelet coefficients and the grey-level co-occurrence matrix form the original feature set, and CFS-based feature selection approach selects the most discriminating feature subset. A trained binary classifier utilizes the selected feature subset to determine whether the acquired image is of real hand or an artefact. For performance evaluation, an antispoofing database—PALMspoof has been developed. This database comprises left- and right-hand images of 104 subjects, and three kinds of artefacts generated from these images. In addition to PALMspoof database, the biometric system’s vulnerability has been assessed on display and print artefacts generated from two publicly available palmprint datasets. Our experimental results show that 1) the palmprint verification system is highly vulnerable with spoof acceptance of 84.56%; 2) the proposed PAD approach is effective against both print and display attacks, in both same-device and cross-device scenarios; and 3) the proposed approach for PAD provides an average improvement of 12.73 percentage points in classification error rate over local binary pattern (LBP)-based PAD approach.     

Artificial neural network approach for LNA design of GPS receiver

Paper presents an ANN modeling of microwave LNA for the global positioning front end receiver, operating at 1.57542 GHz. To design LNA, multilayer perceptron architecture is used. The scattering parameters of LNA are calculated using Levenberg Marquardt Backpropagation Algorithm for the frequency range 100 MHz to 8 GHz. The inputs given to this architecture are drain to source current, drain to source voltage, temperature and frequency and the outputs are maximum available gain, noise figure and scattering parameters (magnitude as well as angle). ANN model is trained using Agilent MGA 72543 GaAs pHEMT Low Noise Amplifier datasheet and this model shows high regression. The smith and polar charts are plotted for frequency range 100 MHz to 8 GHz.