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51.
The Penicillium genus of fungi is a frequently reported cause of allergic reactions. However, only a limited number of allergens have been reported. In Penicillium spp., many allergens show higher IgE-binding activity in culture filtrate extracts than in cellular extracts. In order to investigate the IgE-reactive profile of mold-sensitized patients, secreted IgE-reactive proteins from Penicillium citrinum were identified by 2-DE, serum immunoblotting, and nanoLC-MS/MS. Among the IgE-reactive spots, one known allergen, Pen c 13, and four novel allergens were identified. The cDNAs coding for Pen c 32 and Pen c 30 were cloned using designed primers based on nanoLC-MS/MS analysis. The amino acid sequences of Pen c 32 and Pen c 30 were, respectively, found to have extensive similarity with those of pectate lyases and catalases from various fungi. Native Pen c 30 was shown to have catalase activity and to bind to serum IgE from 48% of mold-allergic patients and induced immediate type skin reactions in a sensitized patient. Here, we present a proteome approach which resulted in the identification of four novel secreted allergens. These novel allergens might be useful in allergy diagnosis and in the treatment of mold-allergic disorders. 相似文献
52.
Chiu YH Wu CH Su HY Cheng CJ 《IEEE transactions on pattern analysis and machine intelligence》2007,29(1):28-39
This work proposes a novel approach to translate Chinese to Taiwanese sign language and to synthesize sign videos. An aligned bilingual corpus of Chinese and Taiwanese sign language (TSL) with linguistic and signing information is also presented for sign language translation. A two-pass alignment in syntax level and phrase level is developed to obtain the optimal alignment between Chinese sentences and Taiwanese sign sequences. For sign video synthesis, a scoring function is presented to develop motion transition-balanced sign videos with rich combinations of intersign transitions. Finally, the maximum a posteriori (MAP) algorithm is employed for sign video synthesis based on joint optimization of two-pass word alignment and intersign epenthesis generation. Several experiments are conducted in an educational environment to evaluate the performance on the comprehension of sign expression. The proposed approach outperforms the IBM Model2 in sign language translation. Moreover, deaf students perceived sign videos generated by the proposed method to be satisfactory 相似文献
53.
Urine is a human specimen that is easily obtained non-invasively for clinical diagnosis. We attempted to enhance the resolution of current human urine proteomes and construct a comprehensive reference database for advanced studies, such as the discovery of biomarkers for renal diseases. Multi-dimensional LC-MS/MS was coupled with de novo sequencing and database matching. The proposed approach improved the identification of not only the proteins, but also the post-translational sites of urinary proteins. We identified 165, 200 and 259 unique gene products in the urine proteomes from males, females and pregnant women, respectively. When all of the results were combined and the redundancies removed, a total of 1095 distinct peptides were identified. Of these, 1016 peptides were associated with 334 unique gene products. In this study, over 100 gene products, including some disease-related proteins, were detected in urine for the first time by proteomic approaches. Various proteins with novel post-translational hydroxylation were identified using the MASCOT program and de novo sequencing. All proteins with peptide information were summarized into a comprehensive urine protein database. We believe that this comprehensive urine proteome database will assist in the identification of urinary proteins/polypeptides whose spectra are difficult to interpret in the discovery of urinary biomarkers. 相似文献
54.
Linear relations have been observed between the reciprocal of the noise ratio and the reciprocal of the output power of the He-Cd laser as well as between the noise ratio and the net gain within the laser cavity. By a feedback method with an acoustooptic modulator in the laser cavity at the Brewster angle for low optical loss, a stable laser with high power output and low noise has been obtained. The noise ratio and the output power are 0.8% and 33 mW, respectively, at 4416 A 相似文献
55.
With the increasing popularity of cloud computing, there is increased motivation to outsource data services to the cloud to save money. An important problem in such an environment is to protect user privacy while querying data from the cloud. To address this problem, researchers have proposed several techniques. However, existing techniques incur heavy computational and bandwidth related costs, which will be unacceptable to users. In this paper, we propose a cooperative private searching (COPS) protocol that provides the same privacy protections as prior protocols, but with much lower overhead. Our protocol allows multiple users to combine their queries to reduce the querying cost while protecting their privacy. Extensive evaluations have been conducted on both analytical models and on a real cloud environment to examine the effectiveness of our protocol. Our simulation results show that the proposed protocol reduces computational costs by 80% and bandwidth cost by 37%, even when only five users query data. 相似文献
56.
This study examined whether evaluations (agreements, disagreements), knowledge content (new ideas, justifications), or social cues (SCs) in recent messages affected a current message’s positive or negative SC during asynchronous, online discussions. Using statistical discourse analysis, we modeled 894 messages by 183 participants on 60 high school mathematics topics (typically eight people posted per topic) on a mathematics problem solving website not connected to any class or school. Results showed that recent agreements increased the likelihood of positive SC, whereas justifications reduced it. Disagreements increased the likelihood of negative SC, whereas new ideas reduced it. Meanwhile, recent positive or negative SCs did not affect the likelihood of a subsequent SC. Together, these results suggest that judicious use of positive SCs rather than negative SCs during disagreements can help students both construct knowledge and maintain social relationships. 相似文献
57.
Du-Ming Tsai Ming-Chun Chen Wei-Chen Li Wei-Yao Chiu 《Machine Vision and Applications》2012,23(5):869-886
In this paper, we propose a fast regularity measure for defect detection in non-textured and homogeneously textured surfaces, with specific emphasis on ill-defined subtle defects. A small neighborhood window of proper size is first chosen and they slide over the entire inspection image in a pixel-by-pixel basis. The regularity measure for each image patch enclosed in the window is then derived from the eigenvalues of the covariance matrix formed by the variance–covariance of the x- and y-coordinates with the pixel gray levels as the weights for all pixel points in the window. The two eigenvalues of the weighted covariance matrix will be approximately the same when the image patch contains only a homogeneous region, whereas the two eigenvalues will be relatively different if the image patch in the window contains a defect. The smaller eigenvalue of the covariance matrix is then used as the regularity measure. The integral image technique is introduced to the computation of the regularity measure so that it is invariant to the neighborhood window size. The proposed method uses only one single discrimination feature for defect detection. It avoids the use of complicated classifiers in a high-dimensional feature space, and requires no learning process from a set of defective and defect-free training samples. Experimental results on a variety of material surfaces found in industry, including textured images of plastic surfaces and leather and non-textured images of backside solar wafers and LCD backlight panels, have shown the effectiveness of the proposed regularity measure for surface defect detection. It is computationally very fast, and takes only 0.032 s for a 400 × 400 image on a Pentium 3.00?GHz personal computer. In a test set of 73 backside solar wafer images involving 53 defect-free and 20 defective samples, the proposed regularity measure can correctly identify all the test images. 相似文献
58.
Singa Wang ChiuYuan-Shyi Peter Chiu Jyh-Chau Yang 《Expert systems with applications》2012,39(3):2578-2583
This paper combines an alternative multi-delivery policy into an imperfect economic production quantity (EPQ) model with partial rework, with the purpose of reducing supplier’s stock holding cost. We extend the problem examined by Chiu et al. [Chiu, Y.-S. P, Chiu, S. W., Li, C.-Y., & Ting, C.-K. (2009). Incorporating multi-delivery policy and quality assurance into economic production lot size problem. Journal of Scientific and Industrial Research, 68(6), 505-512] by considering an n + 1 delivery policy in lieu of n multi-delivery plan for the specific EPQ model with partial rework. Under such a policy, an initial delivery of perfect (finished) items is distributed to customer for satisfying product demand during manufacturer’s regular production time and rework time. At the end of rework, fixed quantity n installments of the finished products are delivered to customer at a fixed interval of time. As a result, a closed-form optimal replenishment batch size solution to the problem is obtained. A numerical example with analysis and comparison is provided to show practical usage of the proposed model and demonstrate its significant savings in stock holding cost. 相似文献
59.
Chiu Liu 《Canadian Metallurgical Quarterly》2005,131(2):161-166
Van der Waals force between neutral molecules is employed to characterize the interaction among molecules in asphalt cement. Several important consequences emerge from the consideration. The brittle strength of the asphalt binder is shown to be linked to the well depth of the Van der Waals potential and the mesoscopic cracks present within the asphalt binder. Moreover, the elastic modulus of asphalt binder is analytically related to the potential well depth. The strength of the asphalt concrete (AC) is estimated by considering aggregate surface characteristics and the adhesion strength between asphaltenes or resins and molecules of aggregates. These predictions can help design asphalt concrete pavement. Formulas for predicting the binder strength and the interfacial breaking strength between aggregates and binders are derived. These results are supported by reported data. Furthermore, an analytic expression for the strength of AC is given at temperature below the AC glass transition point. 相似文献
60.
A case-based expert support system for due-date assignment in a wafer fabrication factory 总被引:3,自引:1,他引:3
Chaochang Chiu Pei-Chann Chang Nan-Hsing Chiu 《Journal of Intelligent Manufacturing》2003,14(3-4):287-296
Owing to the complexity of wafer fabrication, the traditional human approach to assigning due-date is imprecise and very prone to failure, especially when the shop status is dynamically changing. Therefore, assigning a due date to each order becomes a challenge to the production planning and scheduling staff. Since most production orders are similar to those previously manufactured, the case based reasoning (CBR) approach provides a suitable means for solving the due-date assignment problem. This research proposes a CBR approach that employs the k-nearest neighbors concept with dynamic feature weights and non-linear similarity functions. The test results show that the proposed approach can more accurately predict order due dates than other approaches. 相似文献