Interferometric analysis of reorientational nonlinear phenomena at 10.6 microm in a nematic liquid crystal

We describe an infrared interferometric technique based on a two-dimensional spatial fringe analysis Fourier method for investigating the characteristic ring diffraction pattern generated by the self-phase-modulation effect induced in nematic liquid crystals (NLCs) by an infrared laser beam and for measuring the nonlinear refractive index of the NLCs. The experimental setup employs a Mach-Zehnder interferometer with a cw CO2 laser emitting at 10.6 microm and a pyroelectric optoelectronic sensor matrix to detect the modulated ring-pattern intensity distribution formed in the far field by a nematic E7 sample. A Fourier-transform-based analysis of the interference fringe pattern allows comparison of the measurements with the theoretical ring-pattern intensity distribution. We show that accurate determination of the nonlinear refractive index can be obtained by analyzing the two-dimensional phase distribution of the modulated ring pattern.     

Inhibitory effect of umbelliferone aminoalkyl derivatives on oxidosqualene cyclases from S. cerevisiae, T. cruzi, P. carinii, H. sapiens, and A. thaliana: a structure-activity study

Eighteen coumarin derivatives were tested as inhibitors of oxidosqualene cyclases (OSCs) from Saccharomyces cerevisiae, Trypanosoma cruzi, Pneumocystis carinii, Homo sapiens, and Arabidopsis thaliana, all expressed in an OSC-defective strain of S. cerevisiae.35 All the compounds have an aminoalkyl chain bound to an aromatic nucleus; unconventional synthetic procedures (microwave- and ultrasound-promoted reactions) were successfully used to prepare some of them. The most interesting structure-dependent difference in inhibitory activities was observed with an N-oxide group replacement of the tertiary amino group at the end of the side chain. An interesting species specificity also emerged: T. cruzi OSC was the least sensitive enzyme; P. carinii and A. thaliana OSCs were the most sensitive. The remarkable activities of three compounds on the T. cruzi enzyme and of five of them on the P. carinii enzyme suggest the present series as a promising compound family for the development of novel antiparasitic agents.     

New Insights on Metabolic and Genetic Basis of Migraine: Novel Impact on Management and Therapeutical Approach

Migraine is a hereditary disease, usually one-sided, sometimes bilateral. It is characterized by moderate to severe pain, which worsens with physical activity and may be associated with nausea and vomiting, may be accompanied by photophobia and phonophobia. The disorder can occur at any time of the day and can last from 4 to 72 h, with and without aura. The pathogenic mechanism is unclear, but extensive preclinical and clinical studies are ongoing. According to electrophysiology and imaging studies, many brain areas are involved, such as cerebral cortex, thalamus, hypothalamus, and brainstem. The activation of the trigeminovascular system has a key role in the headache phase. There also appears to be a genetic basis behind the development of migraine. Numerous alterations have been identified, and in addition to the genetic cause, there is also a close association with the surrounding environment, as if on the one hand, the genetic alterations may be responsible for the onset of migraine, on the other, the environmental factors seem to be more strongly associated with exacerbations. This review is an analysis of neurophysiological mechanisms, neuropeptide activity, and genetic alterations that play a fundamental role in choosing the best therapeutic strategy. To date, the goal is to create a therapy that is as personalized as possible, and for this reason, steps forward have been made in the pharmacological field in order to identify new therapeutic strategies for both acute treatment and prophylaxis.     

Iron Content,Iron Speciation and Phycocyanin in Commercial Samples of Arthrospira spp.

Cyanobacteria are characterized by high iron content. In this research, we collected ten commercial samples of Arthrospira spp. sold as food supplement to determine iron content and assess whether iron speciation showed variability among samples and changed respect to A. platensis grown in controlled conditions. Particular attention was also paid to phycocyanin, as an iron-binding protein. In six of the ten samples, 14 essential and non-essential trace elements were analysed using ICP-MS. Iron content measured in samples using atomic absorption spectrometry (AAS) varied from 353 (sample S5) to 1459 (sample S7) µg g⁻¹ dry weight and was in the range of those reported by other authors in commercial supplements. Iron speciation was studied using size exclusion chromatography followed by the analysis of the collected fraction for the determination of iron by AAS and for protein separation using SDS-PAGE. Overlapping chromatographic profiles were obtained for total proteins, phycocyanin and iron, although quantitative differences were evidenced among the samples analysed. In most samples, iron was mainly bound to ligands with high molecular mass; however, in four samples iron was also bound to ligands with low molecular mass. In fractions containing the most relevant iron burden, the principal protein was phycocyanin, confirming its role as an iron-binding protein in commercial samples.     

Establishing quality management systems for European food composition databases

As part of the activities of EuroFIR (European Food Information Resource Network), the scientific validation and exploitation of food composition data will be assured by a quality framework. This paper outlines the processes and procedures implemented during the initial development of this quality framework for national food composition data compilers and for laboratories generating new data. EuroFIR’s strategic quality objectives are based upon requirements of national food composition data compilers, laboratories, and users/stakeholders. To implement thorough quality principles, a range of standard procedures and tools are being developed. Elements of a quality management system include criteria for quality control and quality assurance of the compilation process and the computerised systems in which data are hosted, as well as data quality assessment models. The results and findings obtained so far for the proposed quality framework indicate that existing guidelines and standards for laboratories should be updated to meet EuroFIR requirements. The generic compilation process has been described, including the identification of critical stages of the data compilation process and standard operating procedures are being prepared. A data quality assessment system has been developed for application to all nutrients and has been tested by national compilers within the EuroFIR network. The development of the EuroFIR quality policy through open and constructive discussions among food composition data compilers, laboratories, and users, strengthens the proposed quality standards and procedures and is fundamental to improving quality of data exchanged across Europe and beyond.     

The Application of NIR FT-Raman Spectroscopy to Monitor Starch Retrogradation and Crumb Firmness in Semolina Bread

In this study, the potential application of near-infrared (NIR) Fourier-transform (FT)-Raman spectroscopy to monitor starch retrogradation in stored bread crumb was investigated. Semolina-based bread was made and cut into slices, which were stored under controlled conditions in sealed plastic bags. The aging of the bread crumb was monitored by both NIR FT-Raman spectroscopy and a texture analysis over a period of 20 days. Two-dimensional correlation analysis in the spectral range of 390–975 cm⁻¹ revealed characteristic differences among the spectra collected over time for bands that peaked at 480, 765, and 850 cm⁻¹. The band at 480 cm⁻¹ is studied here in detail. During the storage, the peak frequency of this band shifted towards lower wavenumbers, and its full width at half height decreased. Both of these parameters are highly correlated (R ² = 0.921 and R ² = 0.949, respectively) to crumb hardness measured by the texture analyzer.     

Lightweight design and crash analysis of composite frontal impact energy absorbing structures

Carbon fibre composites have shown to be able to perform extremely well in the case of a crash and are being used to manufacture dedicated energy-absorbing components, both in the motor sport world and in constructions of aerospace engineering. While in metallic structures the energy absorption is achieved by plastic deformation, in composite ones it relies on the material diffuse fracture. The design of composite parts should provide stable, regular and controlled dissipation of kinetic energy in order to keep the deceleration level as least as possible. That is possible only after detailed analytical, experimental and numerical analysis of the structural crashworthiness.This paper is presenting the steps to follow in order to design specific lightweight impact attenuators. Only after having characterised the composite material to use, it is possible to model and realise simple CFRP tubular structures through mathematical formulation and explicit FE code LS-DYNA. Also, experimental dynamic tests are performed by use of a drop weight test machine.Achieving a good agreement of the results in previously mentioned analyses, follows to the design of impact attenuator with a more complex geometry, as a composite nose cone of the Formula SAE racing car. In particular, the quasi-static test is performed and reported together with numerical simulation of dynamic stroke. In order to initialize the collapse in a stable way, the design of the composite impact attenuator has been completed with a trigger which is consisted of a very simple smoothing (progressive reduction) of the wall thickness. Initial requirements were set in accordance with the 2008 Formula SAE rules and they were satisfied with the final configuration both in experimental and numerical crash analysis.     

Autophagy in Prostate Cancer and Androgen Suppression Therapy

The role of autophagy is known to be highly complex and context-dependent, leading to both cancer suppression and progression in several tumors including melanoma, breast and prostate cancer. In the present review, recent advances in an understanding of the involvement of autophagy in prostate cancer treatment are described. The regulatory effects of androgens on prostate cancer cell autophagy are particularly discussed in order to highlight the effects of autophagy modulation during androgen deprivation. A critical evaluation of the studies examined in the present review suggests the attractive possibility of autophagy inhibition combined with hormonal therapy as a promising approach for prostate cancer treatment.     

Innovative films with tunable permeability for fresh vegetable packaging applications

Blends of a commercial polypropylene (PP), typically used as food contact material, with increasing amounts of poly(4‐methyl‐1‐pentene) (PMP) are prepared in a twin‐screw extruder to obtain thin films with improved gas permeation for fresh vegetable packaging. Permeability, haze, and transparency of monolayer films produced by casting are investigated under conditions mimicking the industrial ones. O₂ and CO₂ permeability can be tuned by increasing PMP content in the blends from 5 to 20 wt %. Transparency and haze are practically unchanged in the presence of low content of PMP. Moreover, tensile and rheological tests show that PMP does not significantly affect the related properties of blends compared to the neat PP matrix, thus allowing for the industrial applicability of these new formulations. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2014 , 131, 39503.