Overlapping Yee FDTD Method on Nonorthogonal Grids

We propose a new overlapping Yee (OY) method for solving time-domain Maxwell’s equations on nonorthogonal grids. The proposed method is a direct extension of the Finite-Difference Time-Domain (FDTD) method to irregular grids. The OY algorithm is stable and maintains second-order accuracy of the original FDTD method, and it overcomes the late-time instability of the previous FDTD algorithms on nonorthogonal grids. Numerical examples are presented to illustrate the accuracy, stability, convergence and efficiency of the OY method.     

嘴硬，还是命硬？众多游戏大鳄的冬日骄阳

国内“过冬论”甚嚣尘上，甚至有网友笑言：“现在身价亿万也好，工薪一族也罢，无一不是抱着脑袋过日子，大家都生怕一不留神被飞来的“危机”砸得头破血流。”然而在一片御寒之声中，民族网游大鳄们却是围炉而坐，笑谈暖冬。     

Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases

Expression of recombinant proteins as translational fusionsis commonly employed to enhance stability, increase solubilityand facilitate purification of the desired protein. In general,such fusion proteins must be cleaved to release the mature proteinin its native form. The usefulness of the procedure dependson the efficiency and precision of cleavage and its cost perunit activity. We report here the development of a general procedurefor precise and highly efficient cleavage of recombinant fusionproteins using the protease chymosin. DNA encoding a modifiedpro-peptide from bovine chymosin was fused upstream of hirudin,carp growth hormone, thioredoxin and cystatin coding sequencesand expressed in a bacterial Escherichia coli host. Each ofthe resulting fusion proteins was efficiently cleaved at thejunction between the pro-peptide and the desired protein bythe addition of chymosin, as determined by activity, N-terminalsequencing and mass spectrometry of the recovered protein. Thesystem was tested further by cleavage of two fusion proteins,cystatin and thioredoxin, sequestered on oilbody particles obtainedfrom transgenic Arabidopsis seeds. Even when the fusion proteinwas sequestered and immobilized on oilbodies, precise and efficientcleavage was obtained. The precision, efficiency and low costof this procedure suggest that it could be used in larger scalemanufacturing of recombinant proteins which benefit from expressionas fusions in their host organism. Received June 5, 2003; revised August 1, 2003; accepted August 20, 2003.     

Immobilisation of Semliki forest virus for atomic force microscopy

Semliki Forest virus (SFV), an alphavirus, is a single-stranded positive-sense RNA virus. The RNA genome is surrounded by a protein shell known as the capsid which itself is surrounded by a lipid envelope of host cell origin. In this study, SFV strain L10 enveloped virus and its capsid were immobilised onto silicon wafer supports which had been pre-coated with a monolayer of the relevant anti-viral antibody. After drying, the samples were imaged in air, using non-contact mode atomic force microscopy (AFM). Quantification of the AFM images has revealed that both the strain L10 enveloped virus and capsid collapse when immobilised in this manner. The capsid undergoes more significant collapse compared to the enveloped virus. The dimensions of the immobilised enveloped virus and capsid have been compared to a model where the free spherical particles collapse into ellipsoids during immobilisation. For the immobilised capsid the dimensions are consistent with this model whereas for the enveloped virus the model is less effective. The dimensions of the enveloped virus appear to be affected by the antibody used for immobilisation.     

All-Fiber Picosecond Laser System at 1.5 $mu$m Based on Amplification in Short and Heavily Doped Phosphate-Glass Fiber

Amplification of ultrashort pulses in doped fibers is limited by an onset of nonlinear effects in the fiber. At the 1.5-mum wavelength, single-mode fibers typically have anomalous dispersion. The self-phase modulation combined with dispersion leads to instability of multinanojoule pulses in such fibers. Various techniques developed to amplify pulses beyond the nonlinearity limit typically rely on a delicate balance between dispersive and nonlinear effects in different parts of the laser system. We report a simple all-fiber alternative to these complex techniques that utilizes a rapid amplification of pulses in a short and heavily doped phosphate-glass active fiber. In our preliminary experiments, picosecond pulses at 1.5 mum generated by a passively mode-locked fiber oscillator at a repetition rate of 70 MHz are amplified in a 15-cm-long heavily Er-Yb codoped fiber amplifier to the average output power of 1.425 W. The pulse energy and peak power reach 20.4 nJ and 16.6 kW, respectively, while the pulse distortion is minimal in both temporal and spectral domains. Further power up-scaling is possible by using active phosphate fiber with a large mode area, in the amplifier stage     

The simulated environmental impact of incorporating white clover into pasture-based dairy production systems

White clover (WC) offers an alternative source of nitrogen (N) for pasture-based systems. Substituting energy- and carbon-intensive synthetic N fertilizers with N derived from biological fixation by WC has been highlighted as a promising environmental mitigation strategy through the omission of emissions, pollutants, and energy usage during the production and application of synthetic fertilizer. Therefore, the objective was to investigate the effect of the inclusion of WC in perennial ryegrass (PRG) swards on the environmental impact of pasture-based dairy systems. Cradle-to-farm gate life cycle assessment of 3 pasture-based dairy systems were conducted: (1) a PRG–WC sward receiving 150 kg of N/ha per year (CL150), (2) a PRG–WC sward receiving 250 kg of N/ha per year (CL250), and (3) a PRG-only sward receiving 250 kg of N/ha per year (GR250). A dairy environmental model was updated with country-specific N excretion equations and recently developed N₂O, NH₃, and NO₃^? emission factors. The environmental impact categories assessed were global warming potential, nonrenewable energy, acidification potential, and eutrophication potential (marine and freshwater). Impact categories were expressed using 2 functional units: per hectare and per metric tonne of fat- and protein-corrected milk. The GR250 system had the lowest milk production and highest global warming potential, nonrenewable energy, and acidification potential per tonne of fat- and protein-corrected milk for all systems. The CL250 system produced the most milk and had the highest environmental impact across all categories when expressed on an area basis. It also had the highest marine eutrophication potential for both functional units. The impact category freshwater eutrophication potential did not differ across the 3 systems. The CL150 system had the lowest environmental impact across all categories and functional units. This life cycle assessment study demonstrates that the substitution of synthetic N fertilizer with atmospheric N fixed by WC has potential to reduce the environmental impact of intensive pasture-based dairy systems in temperate regions, not only through improvement in animal performance but also through the reduction in total emissions and pollutants contributing to the environmental indicators assessed.     

The volatile profile of longissimus dorsi muscle of heifers fed pasture, pasture silage or cereal concentrate: implication for dietary discrimination

The aim of this study was to determine the effect of different diets on beef meat volatile organic compounds (VOC). Seven heifers grazed pasture for twelve months (group P); 8 heifers received grass silage ad libitum indoors for six months and then were switched to pasture for six months (group SiP); 8 heifers received grass silage ad libitum indoors for six months and then switched to pasture and also offered 0.5 of the diet dry matter of concentrate for six months (group SiPC); 8 heifers received concentrate for twelve months (group C). The muscle longissimus dorsi was sampled at slaughter and subjected to VOC analysis by SPME-GC-MS. Some aldeyhdes, ketones and furans deriving from lipid oxidation were affected by the treatments. Skatole, 3-undecanone, cuminic alcohol and 1-butanol, 2-methyl allowed the discrimination between animals fed concentrate from animals fed non-concentrate diets. Germacrene D, a terpenoid, was a marker of grass feeding.     

Carotenoid, colour and reflectance measurements in bovine adipose tissue to discriminate between beef from different feeding systems

Our hypothesis was that carotenoids in bovine subcutaneous adipose tissue (SAT) together with colour and reflectance spectra could be used to differentiate between beef production systems based on grass, concentrates or combinations thereof. SAT was sampled from the carcasses of heifers fed pasture (P), a barley-based concentrate (C), silage followed by pasture (SiP) or silage followed by pasture with concentrate (SiPC). β-carotene in the SAT from the C group (0.09 μg g(-1)) was lower (P<0.05) than that from the P (0.54 μg g(-1)), SiP (0.49 μg g(-1)) and SiPC (0.49 μg g(-1)) groups. Lutein in the SAT differed (P<0.05) between all groups with 0.13, 0.10, 0.08 and 0.04 μg g(-1) for the P, SiP, SiPC and C groups, respectively. Principal component analysis of the carotenoid data, SAT colour variables ['L', 'a', 'b', 'C', 'H'] and the reflectance data made it possible to distinguish between the animals fed a barley-based concentrate diet and the animals fed pasture-based diets, but not between different pasture-based groups.     

Raman probing of adhesion loss in carbon nanotube – reinforced composite

Raman spectroscopy of a carbon nanotube – reinforced phenolic resin is used to study the interaction of nanotubes with a host matrix. The observed sublinear dependence of the Raman G-band shift on the matrix strain, accompanied by inhomogeneous broadening of the spectral line, is interpreted as a gradual loss of adhesion between nanotubes and the polymer. An approach to simulate the ensemble-averaged Raman response of the nanotubes in composite is proposed, that takes into account nanotube orientation, angular dependence of the polarized Raman response of nanotubes, and adhesion loss between the nanotubes and the polymer. The comparison of the observed Raman line shapes and Raman shifts with simulation provides interesting insights into the micromechanics of nanotube interaction with polymer.     

Global distribution of linear and cyclic volatile methyl siloxanes in air

The global distribution of linear and cyclic volatile methyl silxoanes (VMS) was investigated at 20 sites worldwide, including 5 locations in the Arctic, using sorbent-impregnated polyurethane foam (SIP) disk passive air samplers. Cyclic VMS are currently being considered for regulation because they are high production volume chemicals that are potentially persistent, bioaccumulative, and toxic. Linear and cyclic VMS (including L3, L4, L5, D3, D4, D5, and D6) were analyzed for in air at all urban, background, and Arctic sites. Concentrations of D3 and D4 are significantly correlated, as are D5 and D6, which suggests different sources for these two pairs of compounds. Elevated concentrations of D3 and D4 on the West coast of North America and at high elevation sites suggest these sites are influenced by trans-Pacific transport, while D5 and D6 have elevated concentrations in urban areas, which is most likely due to personal care product use. Measured concentrations of D5 were compared to modeled concentrations generated using both the Danish Eulerian Hemispheric Model (DEHM) and the Berkeley-Trent Global Contaminant Fate Model (BETR Global). The correlation coefficients (r) between the measured and modeled results were 0.73 and 0.58 for the DEHM and BETR models, respectively. Agreement between measurements and models indicate that the sources, transport pathways, and sinks of D5 in the global atmosphere are fairly well understood.