A Targeted Releasable Affinity Probe (TRAP) for In Vivo Photocrosslinking

A protein TRAP : The in vivo photocrosslinking of TRAP after its intracellular targeting to a binding sequence on the bait protein stabilizes protein interactions. Because the crosslinker is releasable, simple mass spectrometry can be used to identify the protein binding sites after purification.

     

Adenylate Kinase 4—A Key Regulator of Proliferation and Metabolic Shift in Human Pulmonary Arterial Smooth Muscle Cells via Akt and HIF-1α Signaling Pathways

Increased proliferation of pulmonary arterial smooth muscle cells (PASMCs) in response to chronic hypoxia contributes to pulmonary vascular remodeling in pulmonary hypertension (PH). PH shares numerous similarities with cancer, including a metabolic shift towards glycolysis. In lung cancer, adenylate kinase 4 (AK4) promotes metabolic reprogramming and metastasis. Against this background, we show that AK4 regulates cell proliferation and energy metabolism of primary human PASMCs. We demonstrate that chronic hypoxia upregulates AK4 in PASMCs in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. RNA interference of AK4 decreases the viability and proliferation of PASMCs under both normoxia and chronic hypoxia. AK4 silencing in PASMCs augments mitochondrial respiration and reduces glycolytic metabolism. The observed effects are associated with reduced levels of phosphorylated protein kinase B (Akt) as well as HIF-1α, indicating the existence of an AK4-HIF-1α feedforward loop in hypoxic PASMCs. Finally, we show that AK4 levels are elevated in pulmonary vessels from patients with idiopathic pulmonary arterial hypertension (IPAH), and AK4 silencing decreases glycolytic metabolism of IPAH-PASMCs. We conclude that AK4 is a new metabolic regulator in PASMCs interacting with HIF-1α and Akt signaling pathways to drive the pro-proliferative and glycolytic phenotype of PH.     

Comparative Characteristic of Energy Content Calculating Methods for the furazan series as an example of energetic materials

The significant expenditures for organic synthesis of high-energy materials necessitate an a priori evaluation of prospects for physical-chemical parameters. The most important of these parameters is the heat of formation characterizing this or the other compound suggested for synthesis. This study describes and analyzes the most well-known and widely used schemes and methods for evaluating and predicting the energy capacity of high-energetic materials. The accuracy of calculations is evaluated for different schemes; the limits of their applicability are determined.     

Estimation and prediction of the heats of formation for non-aromatic polynitro compounds on the basis of the QSPR approach

The analysis of “Quantitative Structure-Property Relationship”(QSPR), concerning the heat of formation in the condensed state, is performed for non-aromatic polynitrocompounds. The QSPR approach and our original computer program “EMMA”(Efficient Modelling of Molecular Activity) are used. This approach is based on the construction of optimal linear regression models involving physical-chemical, topological, informational, and substructural indices; it can be used as an alternative to traditional additive schemes for evaluating physical-chemical characteristics of energetic materials. On the basis of the QSPR method, the “structure-heat of formation (ΔH°_f)” relationship is revealed for a data base of non-aromatic polynitrocompounds, and ΔH°_f is predicted for some hypothetic substances.     

A boundary‐perturbation finite element approach for shape optimization

A numerical method is proposed for the efficient solution of shape optimization problems, which combines the boundary perturbation technique and finite element analysis. The method is computationally efficient in that it requires a number of finite element analyses with a fixed geometry, as opposed to standard shape optimization which requires re‐analysis with varying geometry. The application of the method to general shape optimization is considered. In addition, a special optimization scheme is devised for a class of problems governed by linear partial differential equations. The performance of the method is illustrated via an example which involves acoustic wave scattering from an obstacle. Copyright © 2000 John Wiley & Sons, Ltd.     

Breathable polymeric materials based on high-density polyethylene prepared by environmental crazing

This work addresses the preparation of breathable materials based on high-density polyethylene (HDPE) and characterization of their performance. The sustainable HDPE mesoporous materials were prepared by the tensile drawing of the HDPE films in the presence of physically active liquids (PALEs) via the mechanism of environmental intercrystallite crazing (EIC). The prepared mesoporous materials were characterized by the different physicochemical methods such as differential scanning calorimetry (DSC), atomic force microscopy (AFM), low-temperature nitrogen adsorption (LTNA), and so forth. Water vapor transmission rate (WVTR) of the breathable materials can be tailored by varying the tensile strain upon the EIC and is found to be equal to 597–1345 g m⁻² day⁻¹. The breathable HDPE-based materials are characterized by good mechanical and insulating properties as well as by high water entry pressure (~43–47 bar). Hence, the deformation of HDPE films via the EIC mechanism provides an efficient route for the preparation of mesoporous breathable materials based on the low-cost commercial polymers, which can be used as waterproof, protective, subroof, gas storage, and packaging materials. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48567.     

A New Approach to Explaining the Oscillatory Oxidation of Arsenites,Stibnites and Vismutinites Using Magnetic Method

Magnetic and relativistic effects in uranium catalysts, the movement of charged particles under the effect of a uniform electric field and uniform magnetic field were studied in the paper. We have considered various mechanisms oscillating reactions （Models Jabotinsky-Korzukhina, Brusselator, Oregonator and Advanced Oregonator）. The mechanisms of the motion of charged particles under the influence of an electric field and a uniform magnetic field were proposed.