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排序方式： 共有337条查询结果，搜索用时 15 毫秒
281.
Diana Salnikova Varvara Orekhova Andrey Grechko Antonina Starodubova Evgeny Bezsonov Tatyana Popkova Alexander Orekhov 《International journal of molecular sciences》2021,22(16)
Altered mitochondrial function is currently recognized as an important factor in atherosclerosis initiation and progression. Mitochondrial dysfunction can be caused by mitochondrial DNA (mtDNA) mutations, which can be inherited or spontaneously acquired in various organs and tissues, having more or less profound effects depending on the tissue energy status. Arterial wall cells are among the most vulnerable to mitochondrial dysfunction due to their barrier and metabolic functions. In atherosclerosis, mitochondria cause alteration of cellular metabolism and respiration and are known to produce excessive amounts of reactive oxygen species (ROS) resulting in oxidative stress. These processes are involved in vascular disease and chronic inflammation associated with atherosclerosis. Currently, the list of known mtDNA mutations associated with human pathologies is growing, and many of the identified mtDNA variants are being tested as disease markers. Alleviation of oxidative stress and inflammation appears to be promising for atherosclerosis treatment. In this review, we discuss the role of mitochondrial dysfunction in atherosclerosis development, focusing on the key cell types of the arterial wall involved in the pathological processes. Accumulation of mtDNA mutations in isolated arterial wall cells, such as endothelial cells, may contribute to the development of local inflammatory process that helps explaining the focal distribution of atherosclerotic plaques on the arterial wall surface. We also discuss antioxidant and anti-inflammatory approaches that can potentially reduce the impact of mitochondrial dysfunction. 相似文献
282.
Vladimir Neroev Tatyana Malishevskaya Dietmar Weinert Sergei Astakhov Sergey Kolomeichuk Germaine Cornelissen Yana Kabitskaya Elena Boiko Irina Nemtsova Denis Gubin 《International journal of molecular sciences》2021,22(1)
Parameters of 24-h rhythm in intraocular pressure (IOP) were assessed in patients with stable or advanced primary open-angle glaucoma (S-POAG/A-POAG) and referenced to the phase of “marker” circadian temperature rhythm of each patient. Body temperature and IOP were measured over a 72-h span in 115 participants (65 S-POAG and 50 A-POAG). Retinal Ganglion Cell (RGC) damage was assessed by high-definition optical coherence tomography. The 24-h IOP rhythm in A-POAG patients peaked during the night, opposite to the daytime phase position in S-POAG patients (p < 0.0001). The 24-h IOP phase correlated with RGC loss (p < 0.0001). The internal phase shift between IOP and body temperature gradually increased with POAG progression (p < 0.001). Angiotensin converting enzyme Alu-repeat deletion/insertion (ACE I/D) emerged as a candidate gene polymorphism, which may play a role in the alteration of the circadian IOP variability in advanced glaucoma. To conclude, a reliable estimation of the 24-h rhythm in IOP requires the degree of RGC damage to be assessed. In advanced POAG, the 24-h phase of IOP tended to occur during the night and correlated with RGC loss, being progressively delayed relative to the phase of temperature. 相似文献
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284.
Natalya V. Permyakova Tatyana V. Marenkova Pavel A. Belavin Alla A. Zagorskaya Yuriy V. Sidorchuk Elena V. Deineko 《International journal of molecular sciences》2022,23(15)
Targeted DNA integration into known locations in the genome has potential advantages over the random insertional events typically achieved using conventional means of genetic modification. We studied the presence and extent of DNA rearrangements at the junction of plant and transgenic DNA in five lines of Arabidopsis thaliana suspension cells carrying a site-specific integration of target genes. Two types of templates were used to obtain knock-ins, differing in the presence or absence of flanking DNA homologous to the target site in the genome. For the targeted insertion, we selected the region of the histone H3.3 gene with a very high constitutive level of expression. Our studies showed that all five obtained knock-in cell lines have rearrangements at the borders of the integrated sequence. Significant rearrangements, about 100 or more bp from the side of the right flank, were found in all five plant lines. Reorganizations from the left flank at more than 17 bp were found in three out of five lines. The fact that rearrangements were detected for both variants of the knock-in template (with and without flanks) indicates that the presence of flanks does not affect the occurrence of mutations. 相似文献
285.
286.
Tatyana Savchenko Evgeny Degtyaryov Yaroslav Radzyukevich Vlada Buryak 《International journal of molecular sciences》2022,23(23)
For immobile plants, the main means of protection against adverse environmental factors is the biosynthesis of various secondary (specialized) metabolites. The extreme diversity and high biological activity of these metabolites determine the researchers’ interest in plants as a source of therapeutic agents. Oxylipins, oxygenated derivatives of fatty acids, are particularly promising in this regard. Plant oxylipins, which are characterized by a diversity of chemical structures, can exert protective and therapeutic properties in animal cells. While the therapeutic potential of some classes of plant oxylipins, such as jasmonates and acetylenic oxylipins, has been analyzed thoroughly, other oxylipins are barely studied in this regard. Here, we present a comprehensive overview of the therapeutic potential of all major classes of plant oxylipins, including derivatives of acetylenic fatty acids, jasmonates, six- and nine-carbon aldehydes, oxy-, epoxy-, and hydroxy-derivatives of fatty acids, as well as spontaneously formed phytoprostanes and phytofurans. The presented analysis will provide an impetus for further research investigating the beneficial properties of these secondary metabolites and bringing them closer to practical applications. 相似文献
287.
288.
Maela Manzoli George Avgouropoulos Tatyana Tabakova Joan Papavasiliou Theophilos Ioannides Flora Boccuzzi 《Catalysis Today》2008,138(3-4):239
Nanosized gold catalysts supported on doped ceria were prepared by deposition–precipitation method. A deep characterization study by HRTEM/EDS, XRD, FT-Raman, TPR and FTIR was undergone in order to investigate the effect of ceria modification by various cations (Sm3+, La3+ and Zn2+) on structural and redox properties of gold catalysts. Doping of ceria affected in different way catalytic activity towards purification of H2 via preferential CO oxidation. The following activity order was observed: Au/Zn–CeO2 > Au/Sm–CeO2 > Au/CeO2 > Au/La–CeO2. The differences in CO oxidation rates were ascribed to different concentration of metallic gold particles on the surface of Au catalysts (as confirmed by the intensity of the band at 2103 cm−1 in the FTIR spectra collected during CO–O2 interaction). Gold catalysts on modified ceria showed improved tolerance towards the presence of CO2 and H2O in the PROX feed. The spectroscopic experiments evidence enhanced reactivity when PROX is performed in the presence of H2O already at 90 K. 相似文献
289.
290.
Magdalena Wujak Christine Veith Cheng-Yu Wu Tessa Wilke Zeki Ilker Kanbagli Tatyana Novoyatleva Andreas Guenther Werner Seeger Friedrich Grimminger Natascha Sommer Ralph Theo Schermuly Norbert Weissmann 《International journal of molecular sciences》2021,22(19)
Increased proliferation of pulmonary arterial smooth muscle cells (PASMCs) in response to chronic hypoxia contributes to pulmonary vascular remodeling in pulmonary hypertension (PH). PH shares numerous similarities with cancer, including a metabolic shift towards glycolysis. In lung cancer, adenylate kinase 4 (AK4) promotes metabolic reprogramming and metastasis. Against this background, we show that AK4 regulates cell proliferation and energy metabolism of primary human PASMCs. We demonstrate that chronic hypoxia upregulates AK4 in PASMCs in a hypoxia-inducible factor-1α (HIF-1α)-dependent manner. RNA interference of AK4 decreases the viability and proliferation of PASMCs under both normoxia and chronic hypoxia. AK4 silencing in PASMCs augments mitochondrial respiration and reduces glycolytic metabolism. The observed effects are associated with reduced levels of phosphorylated protein kinase B (Akt) as well as HIF-1α, indicating the existence of an AK4-HIF-1α feedforward loop in hypoxic PASMCs. Finally, we show that AK4 levels are elevated in pulmonary vessels from patients with idiopathic pulmonary arterial hypertension (IPAH), and AK4 silencing decreases glycolytic metabolism of IPAH-PASMCs. We conclude that AK4 is a new metabolic regulator in PASMCs interacting with HIF-1α and Akt signaling pathways to drive the pro-proliferative and glycolytic phenotype of PH. 相似文献