Stimulation of phosphorylation of mitogen-activated protein kinase by 1alpha,25-dihydroxyvitamin D3 in promyelocytic NB4 leukemia cells: a structure-function study

The conformations of isolated rabbit fast myosin light chains (LCs) were modified using trifluoperazine (TFP), the hydrophobic calmodulin inhibitor. CD spectroscopy showed that TFP altered secondary structural content of the LCs, with half-maximal effects at TFP concentrations of approximately 14-50 microM, which is within the range required to alter muscle fiber contraction in both agonistic and antagonistic ways [Kurebayashi, N. & Ogawa, Y. (1988) J. Physiol. 403, 407-424]. EPR spectroscopy provided structural information from paramagnetic probes on C-terminal domain surfaces. In the absence of TFP, tauR (rotational correlation time) was 1.6 ns for both alkali light chains (ALCs) and 1.8 ns for light chain 2 (LC2). This was faster than expected for proteins of this size (approximately 10 ns). TFP progressively recruited the probes into populations with tauR sevenfold to 12-fold slower, with half-maximal effects at a TFP concentration of approximately 370-800 microM. The differences probably indicate that CD spectroscopy detects changes in protein conformation due to 'specific' TFP binding at the LC hydrophobic core, while less specific binding at higher TFP concentrations is required to effect conformational changes on the protein surfaces near the paramagnetic probes. TFP binding was generally not cooperative. Comparative sequence analysis between calmodulin, troponin C, and myosin LCs indicated considerable conservation between residues expected to bind TFP.     

Development of automatic fault point locating and sectional isolating system for power distribution line

This paper describes the development of the Automatic Fault Point Locating and Sectional Isolating System. This system locates a single-line-to-ground fault point at each pole by using ground-current sensors, and isolates the faulted section by controlling pole switches with no outage of unfaulted sections. The ground-current sensor is connected to the line between allied ground terminals of pole devices and grounded conductor. It detects a ground fault current of more than 0.64 amperes that is determined so as to avoid an unexpected operation by stray current, but to detect the fault current accurately, and sends out an emergency signal with time delay of 0.1 s. Sectional isolation starts when both the emergency signal from the sensor and relay operation signals from a substation are received and ends within a feeder circuit-breaker tripping time of 1.5 s.     

Suppression of Active Oxidation of Polycarbosilane-Derived Silicon Carbide Fibers by Preoxidation at High Oxygen Pressure

Three types of polycarbosilane-derived SiC fibers (Nicalon, Hi-Nicalon, and Hi-Nicalon S) with different SiO₂ film thicknesses ( b ) were subjected to exposure tests at 1773 K in an argon-oxygen gas mixture with an oxygen partial pressure of 1 Pa. The suppression effect of a SiO₂ coating on active oxidation was examined through TG, XRD analysis, SEM observation, and tensile tests. All the as-received fibers were oxidized in the active-oxidation regime. The mass gain and the SiO₂ film development showed a suppression of active oxidation at b values of ≧0.070 μm for Nicalon, ≧0.013 μm for Hi-Nicalon, and ≧0.010 μm for Hi-Nicalon S fibers. Considerable strength was retained in the SiO₂-coated fibers. For Hi-Nicalon fibers, the retained strength was 71%–90% of the strength in the as-received state (2.14–2.69 GPa).     

Experiments and Predictions of Soil Desaturation by Air-Injection Technique and the Implications Mediated by Multiphase Flow Simulation

Measures preventing an earthquake-induced soil liquefaction are of significant importance to mitigate the liquefaction hazards. An air-injection technique may be a simple, inexpensive method - this leads the saturated soils to the desaturated by injecting pressurized air, resulting in a higher liquefaction strength and lower susceptibility. The objective of this study is to investigate the evolution of desaturation process during air injection into saturated soil deposits and verify the validity of a multiphase flow simulator if it is capable of being applied for predicting the process as well as the distribution of degree of saturation after the air injection ceased. In this study simplified model tests that simulate the air injection into saturated soils using air-injection probes, are conducted using two different sizes of soil containers. The experiments using the small container are aimed to examine the nominal rates and magnitudes of the soil desaturation driven by air injection, whilst those with the large container are performed to obtain not only the rates and magnitudes but also the distributions of the desaturated zones within the soil. The results obtained indicate, although clearly depending on the physical properties of targeted soils, that the evolution of desaturation is strongly controlled by the air pressures injected and the soil permeabilities. Numerical analyses are also conducted using a multiphase flow simulator to describe the evolution of the soil desaturation, and to examine the applicability of the model as a prediction tool enabling an evolution of desaturation in situ to be followed with time and space. Predictions show a relatively good agreement with the experimental measurements regarding the rates, magnitudes, and distribution of desaturation specifically for the small-container experiments although predictions of desaturated domain slightly overestimate the measurements for the large-container experiments. Thus, this study indicates that the numerical model described is applicable to field problems when the soil properties in terms of flow transport are well-constrained.     

Polymerization of trithiane

The catalytic solid-state polymerization of trithane to polythiomethylene has been studied. The polymerization can be initiated by wide varieties of substances such as metal salts, halogen, and active organic halides. The polymerization proceeds in solid-state without topochemical process. The catalytic irreversible degradation of polythiomethylene to dimethyl sulfide and carbon disulfide has been observed.     

Lorentzian force type fibre-optic AC magnetic sensor realisingfrequency-independent sensitivity

A Lorentzian force type fibre-optic magnetic sensor is reported attaining frequency-independent sensitivity in the range 1 Hz to 10 kHz with minimum detectable field down to 50 nT. It is also shown that this technique can introduce frequency superimposition, which enables measurement of AC magnetic fields at different points simultaneously with one interferometer     

Association of an acute reduction in lipoprotein(a) with coronary artery restenosis after percutaneous transluminal coronary angioplasty

BACKGROUND: Lipoprotein(a) [Lp(a)], which structurally resembles-tissue-type plasminogen, is reported to be associated with coronary atherosclerosis. We examined whether the acute change in Lp(a) by percutaneous transluminal coronary angioplasty (PTCA) is related to restenosis after PTCA. METHODS AND RESULTS: We measured serum Lp(a) and other lipid parameters (triglycerides and total, LDL, and HDL cholesterol) before and 1 day after PTCA in 143 procedures and 3 days after and 4 months after PTCA in 62 procedures. Quantitative coronary angiography was performed, and restenosis was defined according to three criteria: (1) clinical recurrence of ischemic symptoms, (2) a final stenosis > 50%, and (3) an absolute decrease in minimal lumen diameter > 1/2 of the acute gain in the dilated segment. Restenosis was recognized in 25.9%, 35.7%, and 38.5% of the cases 4 months after PTCA for each criterion, respectively. Although triglyceride and LDL, HDL, and total cholesterol levels were similar in the restenosis and no-restenosis groups before PTCA, Lp(a) was significantly higher in the restenosis group. We found a significant reduction in Lp(a) in the restenosis but not the no-restenosis group 1 day after PTCA. At 3 days after and 4 months after PTCA, Lp(a) was similar in the two groups. A multivariate-analysis revealed that the absolute change in Lp(a) (before versus 1 day after PTCA) to be the sole significant predictor of restenosis among the clinical, angiographic, and plasma lipid parameters examined. CONCLUSIONS: Lp(a) levels were significantly higher in the restenosis group, and they fell significantly after PTCA in the restenosis group.     

Alternation in the coronary blood flow velocity pattern in patients with no reflow and reperfused acute myocardial infarction

1. The effects of fluvastatin, a new 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, on the vascular angiotensin converting enzyme (ACE) activity in hyperlipidaemic rabbits were compared with those of enalapril, an ACE inhibitor. 2. Rabbits were fed a 1.5% cholesterol containing diet or normal diet for 16 weeks and treated with either fluvastatin or enalapril in the diet at the respective doses of 2 and 10 mg kg-1 day-1. The total cholesterol, triglyceride and phospholipid levels in serum were significantly increased in rabbits fed the high cholesterol diet. Treatment with fluvastatin but not enalapril resulted in a decrease in serum lipids. 3. The vascular ACE activities assessed via the cleavage rate from synthetic substrate in the aortic arches and upper thoracic aortae were increased by 8 to 10 times when the rabbits were made hyperlipidaemic. Fluvastatin as well as enalapril significantly lowered the tissue ACE in the aortae. 4. The ACE activities in serum did not alter in hyperlipidaemic rabbits either in the presence or absence of fluvastatin. The serum ACE activity was lowered by enalapril. 5. The lipid peroxide in serum as well as the plaque area in the thoracic aorta was significantly increased in the cholesterol diet-fed rabbits. Treatment with fluvastatin or enalapril reduced both serum lipid peroxide and plaque formation. The relaxant responses to acetylcoholine (ACh) were significantly suppressed in the cholesterol-fed rabbits. Treatment with fluvastatin or enalapril significantly reversed the suppression of ACh-induced relaxation. 6. It seems that the reduction of vascular ACE is not coupled to lipids and ACE activity in serum, but rather to lipid peroxidation. Thus, the decrease in vascular ACE activity by fluvastatin as well as the lipid-lowering effect may reduce the risk of atherosclerosis progression in the vasculature.     

A 1 ns, 1 W, 2.5 V, 32 Kb NTL-CMOS SRAM macro using a memory cellwith PMOS access transistors

While an ECL-CMOS SRAM can achieve both ultra high speed and high density, it consumes a lot of power and cannot be applied to low power supply voltage applications. This paper describes an NTL (Non Threshold Logic)-CMOS SRAM macro that consists of a PMOS access transistor CMOS memory cell, an NTL decoder with an on-chip voltage generator, and an automatic bit line signal voltage swing controller. A 32 Kb SRAM macro, which achieves a 1 ns access time at 2.5 V power supply and consumes a mere 1 W, has been developed on a 0.4 μm BiCMOS technology