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71.
Chiba T Wauke T Sadamasu K Yano K Morozumi S 《Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan》2007,48(1):1-7
The identification of 20 strains of yeasts isolated from foods by means of DNA sequence analysis with two kinds of universal primers for the rDNA region was examined, and the results were compared with those of the conventional phenotyping test using API 20C AUX. In the analysis of the 26S region, all 20 yeast strains tested were identified at the species level. In the ITS1 region, 16 strains were also classified at the species level. In addition, all results of DNA sequence analysis were consistent with those of the phenotyping test at the genus level. Furthermore, DNA sequence analysis was able to identify causative yeasts observed in two suspect foods, though phenotyping tests alone failed to identify them. 相似文献
72.
73.
Guisheng Zhai Shohei Okuno Joe Imae Tomoaki Kobayashi 《Journal of Intelligent and Robotic Systems》2011,63(2):309-322
In this paper, we study a consensus problem for multi-agent systems via dynamic output feedback control. The entire system is decentralized in the sense that each agent can only obtain output information from its neighbor agents. For practical purpose, we assume that actuator limitation exists, and require that the consensus be achieved among the agents at a specified convergence rate. By using an appropriate coordinate transformation, we reduce the consensus problem on hand to solving a strict matrix inequality, and then propose to use the homotopy based method for solving the matrix inequality. It turns out that our algorithm includes the existing graph Laplacian based algorithm as a special case. 相似文献
74.
Tokuyuki Yoshida Yasuo Yoshioka Hideki Takahashi Kazuki Misato Takahide Mori Toshiro Hirai Kazuya Nagano Yasuhiro Abe Yohei Mukai Haruhiko Kamada Shin-ichi Tsunoda Hiromi Nabeshi Tomoaki Yoshikawa Kazuma Higashisaka Yasuo Tsutsumi 《Nanoscale research letters》2014,9(1):532
Although amorphous silica nanoparticles are widely used in the production of food products (e.g., as anticaking agents), there is little information available about their absorption and biological effects after oral exposure. Here, we examined the in vitro intestinal absorption and in vivo biological effects in mice of orally administered amorphous silica particles with diameters of 70, 300, and 1,000 nm (nSP70, mSP300, and mSP1000, respectively) and of nSP70 that had been surface-modified with carboxyl or amine groups (nSP70-C and nSP70-N, respectively). Analysis of intestinal absorption by means of the everted gut sac method combined with an inductively coupled plasma optical emission spectrometer showed that the intestinal absorption of nSP70-C was significantly greater than that of nSP70. The absorption of nSP70-N tended to be greater than that of nSP70; however, the results were not statistically significant. Our results indicate that silica nanoparticles can be absorbed through the intestine and that particle diameter and surface properties are major determinants of the degree of absorption. We also examined the biological effects of the silica particles after 28-day oral exposure in mice. Hematological, histopathological, and biochemical analyses showed no significant differences between control mice and mice treated with the silica particles, suggesting that the silica nanoparticles evaluated in this study are safe for use in food production. 相似文献
75.
Recovery of liquid fuel from hydrocarbon-rich microalgae by thermochemical liquefaction 总被引:11,自引:0,他引:11
Yutaka Dote Shigeki Sawayama Seiichi Inoue Tomoaki Minowa Shin-ya Yokoyama 《Fuel》1994,73(12):1855-1857
Liquefaction of Botryococcus braunii, a colony-forming microalga, with high moisture content was performed with or without sodium carbonate as a catalyst for conversion into liquid fuel and recovery of hydrocarbons. A greater amount of oil than the content of hydrocarbons in B. braunii (50 wt% db) was obtained, in a yield of 57–64 wt% at 300 °C. The oil was equivalent in quality to petroleum oil. The recovery of hydrocarbons was a maximum (>95%) at 300 °C. 相似文献
76.
Hiroaki Takeda Tomoaki Kuze Takashi Nishida Kiyoshi Uchiyama Tadashi Shiosaki 《Materials Research Bulletin》2008,43(7):1731-1736
The influences of aluminum substitution for gallium in the langasite-type La3Nb0.5Ga5.5O14 (LNG) crystals on their growth and electric properties were investigated. Al-substituted LNG (La3Nb0.5Ga5.5−xAlxO14; LNGAx) single crystals up to the solubility limit x = 0.2 have been grown by the conventional Czochralski technique. The electric properties of the LNGAx crystals were investigated and compared to those of LNG. With Al substitution, the piezoelectric constants, d11 and d14, were slightly higher. The LNGAx crystals showed a temperature dependence of d11 similar to that of the LNG crystal. 相似文献
77.
Nabeshi H Yoshikawa T Matsuyama K Nakazato Y Arimori A Isobe M Tochigi S Kondoh S Hirai T Akase T Yamashita T Yamashita K Yoshida T Nagano K Abe Y Yoshioka Y Kamada H Imazawa T Itoh N Kondoh M Yagi K Mayumi T Tsunoda S Tsutsumi Y 《Nanotechnology》2012,23(4):045101
We previously reported that well-dispersed amorphous nanosilicas with particle size 70 nm (nSP70) penetrate skin and produce systemic exposure after topical application. These findings underscore the need to examine biological effects after systemic exposure to nanosilicas. The present study was designed to examine the biological effects. BALB/c mice were intravenously injected with amorphous nanosilicas of sizes 70, 100, 300, 1000 nm and then assessed for survival, blood biochemistry, and coagulation. As a result, injection of nSP70 caused fatal toxicity, liver damage, and platelet depletion, suggesting that nSP70 caused consumptive coagulopathy. Additionally, nSP70 exerts procoagulant activity in vitro associated with an increase in specific surface area, which increases as diameter reduces. In contrast, nSP70-mediated procoagulant activity was absent in factor XII-deficient plasma. Collectively, we revealed that interaction between nSP70 and intrinsic coagulation factors such as factor XII, were deeply related to nSP70-induced harmful effects. In other words, it is suggested that if interaction between nSP70 and coagulation factors can be suppressed, nSP70-induced harmful effects may be avoided. These results would provide useful information for ensuring the safety of nanomaterials (NMs) and open new frontiers in biological fields by the use of NMs. 相似文献
78.
Silicon rubber, PDMS, was irradiated by Ar, Ar/H2 and Ar/O2 plasmas to increase the surface wettability to water which was found to increase with the irradiation time for every application of plasma irradiation. The use of Ar/O2 plasma was found to be particularly effective in enhancing the surface wettability. The aging behavior due to exposure to air, ethanol or water was also measured. In the case of continuous exposure to water after plasma treatment, the wettability could be maintained for more than several months with the water immersion. 相似文献
79.
Yasukawa T Nagamine K Horiguchi Y Shiku H Koide M Itayama T Shiraishi F Matsue T 《Analytical chemistry》2008,80(10):3722-3727
A novel microfluidic device with an array of analytical chambers was developed in order to perform single-cell-based gene-function analysis. A series of analytical processes was carried out using the device, including electrophoretic manipulation of single cells and electrochemical measurement of gene function. A poly(dimethylsiloxane) microstructure with a microfluidic channel (150 microm in width, 10 microm in height) and an analytical chamber (100 x 20 x 10 microm (3)) were fabricated and aligned on a glass substrate with an array of Au microelectrodes. Two microelectrodes positioned in the analytical chamber were employed as a working electrode for the electrophoretic manipulation of cells and electrochemical measurements. A yeast strain ( Saccharomyces cerevisiae Y190) carrying the beta-galactosidase reporter gene was used to demonstrate that the device could detect the enzyme. Target cells flowing through the main channel were introduced into the chamber by electrophoresis using the ground electrode laid on the main channel. When the cell was treated with 17beta-estradiol, gene expression was triggered to produce beta-galactosidase, catalyzing the hydrolysis of p-aminophenyl-beta- D-galactopyranoside to form p-aminophenol (PAP). The enzymatically generated PAP was detected by cyclic voltammetry and amperometry at the single-cell level in the chamber of the device. Generator-collector mode amperometry was also applied to amplify the current response originating from gene expression in the trapped single cells. After electrochemical measurement, the trapped cells were easily released from the chamber using electrophoretic force. 相似文献
80.
We have developed the separated pulsed laser deposition (SPLD) technique to prepare high quality ZnO based films exhibiting uniform and droplet-free properties. This SPLD consists of an ablation chamber and a deposition chamber which can be independently evacuated under different ambient gases.The gas species and the pressures in both chambers can be arbitrarily chosen for the specific deposition such as nanostructured films and nanoparticles. The ablation chamber is a stainless steel globe and the deposition chamber is a quartz tube connected to a metallic conic wall with an orifice. We used a KrF excimer laser with λ = 248 nm and 25 ns pulse duration. The different gas conditions in two chambers allow us to realize optimal control of the plasma plume, the gas phase reaction and the film growth by applying the bias voltage between the conic wall and the substrate under the magnetic field. We can expect that at appropriate pressures the electric and magnetic field motion (E × B azimuthal drift velocity) gives significant influences on film growth.We have deposited ZnO thin films at various pressures of ablation chamber (Pab) and deposition chamber (Pd). The deposition conditions used here were laser fluence of 3 J/cm2, laser shot number of 30,000, Pab of 0.67-2.67 Pa (O2 or Ar), Pd of 0.399-2.67 Pa (O2), and substrate temperature of 400 °C. Particle-free and uniform ZnO films were obtained at Pab of 0.67 Pa (Ar) and Pd of 1.33 Pa (O2). The ZnO film showed high preferential orientation of (002) plane, optical band gap of 2.7 eV, grain size of 42 nm and surface roughness of 1.2 nm. 相似文献