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101.
Integrated microfluidic system enabling (bio)chemical reactions with on-line MALDI-TOF mass spectrometry 总被引:2,自引:0,他引:2
Brivio M Fokkens RH Verboom W Reinhoudt DN Tas NR Goedbloed M van den Berg A 《Analytical chemistry》2002,74(16):3972-3976
A continuous flow micro total analysis system (micro-TAS) consisting of an on-chip microfluidic device connected to a matrix assisted laser desorption ionization [MALDI] time-of-flight [TOF] mass spectrometer (MS) as an analytical screening system is presented. Reaction microchannels and inlet/outlet reservoirs were fabricated by powderblasting on glass wafers that were then bonded to silicon substrates. The novel lab-on-a-chip was realized by integrating the microdevice with a MALDI-TOFMS standard sample plate used as carrier to get the microfluidic device in the MALDI instrument. A novel pressure-driven pumping mechanism using the vacuum of the instrument as a driving force induces flow in the reaction microchannel in a self-activating way. Organic syntheses as well as biochemical reactions are carried out entirely inside the MALDI-MS ionization vacuum chamber and analyzed on-line by MALDI-TOFMS in real time. The effectiveness of the micro-TAS system has been successfully demonstrated with several examples of (bio)chemical reactions. 相似文献
102.
103.
This paper deals with the competing risks model as a special case of a multi-state model. The properties of the model are reviewed and contrasted to the so-called latent failure time approach. The relation between the competing risks model and right-censoring is discussed and regression analysis of the cumulative incidence function briefly reviewed. Two real data examples are presented and a guide to the practitioner is given. 相似文献
104.
In this study, sudden load was applied to the trunk and situations with alternating low levels of intra-abdominal pressure (IAP) realistic to work situations were compared. The aim was to see if IAP and the small increases in co-contraction of back muscles that follow are capable of increasing the stiffness of the lumbar spine. Nine subjects participated in ten sudden load situations during which they were asked to hold a box and conduct a percentage of maximal IAP. The hip was fixed and the load was applied horizontally on the trunk. EMG, IAP, and movement of the trunk were measured. It was found that IAP of a size likely to appear in work situations, and the concomitant increase in muscle co-activation increased the spine stiffness. This increase in stiffness decreased the movement caused by the sudden load. These results show that both abdominal- and back muscles may have an important role in stabilising the spine, and in decreasing movements caused by sudden loads likely to appear in numerous work 相似文献
105.
Stig Kildegrd Andersen Henrik Carlsen Per Grove Thomsen 《Simulation Modelling Practice and Theory》2006,14(8):1073
We present an approach for modelling unsteady, primarily one-dimensional, compressible flow. The conservation laws for mass, energy, and momentum are applied to a staggered mesh of control volumes and loss mechanisms are included directly as extra terms. Heat transfer, flow friction, and multidimensional effects are calculated using empirical correlations. Transformations of the conservation equations into new variables, artificial dissipation for dissipating acoustic phenomena, and an asymmetric interpolation method for minimising numerical diffusion and non physical temperature oscillations are presented. The capabilities of the approach are illustrated with an example solution and an experimental validation of a Stirling engine model. 相似文献
106.
Closed-form solution for the Wigner phase-space distribution function for diffuse reflection and small-angle scattering in a random medium 总被引:1,自引:0,他引:1
Yura HT Thrane L Andersen PE 《Journal of the Optical Society of America. A, Optics, image science, and vision》2000,17(12):2464-2474
Within the paraxial approximation, a closed-form solution for the Wigner phase-space distribution function is derived for diffuse reflection and small-angle scattering in a random medium. This solution is based on the extended Huygens-Fresnel principle for the optical field, which is widely used in studies of wave propagation through random media. The results are general in that they apply to both an arbitrary small-angle volume scattering function, and arbitrary (real) ABCD optical systems. Furthermore, they are valid in both the single- and multiple-scattering regimes. Some general features of the Wigner phase-space distribution function are discussed, and analytic results are obtained for various types of scattering functions in the asymptotic limit s > 1, where s is the optical depth. In particular, explicit results are presented for optical coherence tomography (OCT) systems. On this basis, a novel way of creating OCT images based on measurements of the momentum width of the Wigner phase-space distribution is suggested, and the advantage over conventional OCT images is discussed. Because all previous published studies regarding the Wigner function are carried out in the transmission geometry, it is important to note that the extended Huygens-Fresnel principle and the ABCD matrix formalism may be used successfully to describe this geometry (within the paraxial approximation). Therefore for completeness we present in an appendix the general closed-form solution for the Wigner phase-space distribution function in ABCD paraxial optical systems for direct propagation through random media, and in a second appendix absorption effects are included. 相似文献
107.
Determination of RN(-) phenotype in pigs at slaughter-line using visual and near-infrared spectroscopy 总被引:1,自引:0,他引:1
The specific characteristics of meat from the Hampshire breed of pig, including high glycogen content and low ultimate pH and technological yield, have been associated with the dominant RN gene. In Sweden, purebred Hampshire or Hampshire x Yorkshire is often used as terminal sire in the three-way crosses used for pig meat production. For the industry producing cured and cooked hams there is a need to detect the RN(-) carriers of the pigs early post mortem in order to select non-carriers for ham production. In the present study, the possibility of separating RN(-) carriers from non-carriers using a combination of visual and near-infrared (NIR) spectroscopic measurements in reflectance mode through a fibre-optic probe has been studied on commercial pigs in a slaughterhouse. The NIR measurements were performed 30 min post-mortem. Sixty-three animals were considered to be carriers and 33 animals non-carriers based on their glycolytic potential in M. semimembranosus (above 200 μmol/g for RN(-)rn(+) and below 180 μmol/g for rn(+)rn(+)). By using NIR together with classification with neural networks, RN(-) carriers could be separated from non-carriers. None of the carriers and only four non-carriers of the RN gene were misclassified as carriers of the RN gene. The ultimate pH could be predicted using linear partial least squares regression with a correlation coefficient of 0.57 and an accuracy of 0.074 root mean square error of prediction. 相似文献
108.
109.
We present a design for an optical data communications receiver-transmitter pair based on the holographic correction of a large diameter, poor-quality, reflecting primary mirror. The telescope has a narrow bandwidth (<0.1 nm) with good signal frequency isolation (>60 dB) and is scalable to meter-class apertures. We demonstrate the correction of a reflector telescope with over 2000 waves of aberration to diffraction-limited operation, capable of handling data transmission rates up to 100 GHz. 相似文献
110.
Linhjell D. Lundgaard L. Berg G. 《Dielectrics and Electrical Insulation, IEEE Transactions on》1994,1(3):447-458
A 67 mm point-plane gap immersed in transformer oil, at atmospheric pressure, stressed with a 1/180 μs impulse, has been studied by recording gap current and light emission. Time-resolved images were obtained by use of an image converter camera. Minimum breakdown voltages were twice as high for negative as for positive points. All streamers at these and higher voltages were supersonic, with negative streamer velocities ⩽50 km/s. The speed of the positive streamer gradually increased to 19 km/s with increasing voltage, where it saturated. At high stresses the propagating positive streamer has been observed to switch to a faster mode which propagates with speeds in the 65 to 200 km/s range. Positive streamers were bush shaped to 2× the minimum breakdown voltage, the branching diminishing with increased voltage. Negative streamers were coarsely bush shaped or usually tree shaped. For both polarities, light emission consisted of a background light, with superposed pulses corresponding to 15 ns wide current pulses of up to 10 A. The pulses are caused by very bright, brief re-illuminations of single streamer channels. The tips of positive streamers are considerably more luminous than the channels, indicating that electron multiplication may take place at the tips. A model of the streamers as being plasma filled channels may explain the re-illuminations 相似文献