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排序方式: 共有3276条查询结果,搜索用时 15 毫秒
31.
Raquel Alves dos Santos Teresinha Rosa Cabral Isabel Rosa Cabral Lusania Maria Greggi Antunes Cristiane Pontes Andrade Plínio Cerqueira dos Santos Cardoso Marcelo de Oliveira Bahia Claudia Pessoa José Luis Martins do Nascimento Rommel Rodríguez Burbano Catarina Satie Takahashi 《Biocell》2008,32(2):195-200
Physalis angulata L (Solanaceae) is a medicinal plant from North of Brazil, whose different extracts and infusions are commonly used in the popular medicine for the treatment of malaria, asthma, hepatitis, dermatitis and rheumatism. However, the genotoxic effects of P. angulata on human cells is not well known. The main purpose of the present study was to evaluate the in vitro genotoxic effects of aqueous extract of P. angulata using the comet assay and the micronucleus assay in human lymphocytes provided from 6 healthy donors. Treatments with P. angulata extracts were performed in vitro in order to access the extent of DNA damage. The comet assay has shown that treatments with P. angulata at 0.5, 1.0, 2.0, 3.0 and 6.0 microg/mL in culture medium were genotoxic. Lymphocytes treated with P. angulata at the concentrations of 3.0 and 6.0 microg/mL in culture medium showed a statistically significant increase in the frequency of micronucleus (p<0.05), however, the cytokinesis blocked proliferation index (CBPI) was not decreased after P. angulata treatment. In conclusion, the present work demonstrated the genotoxic effects of P. angulata extract on human lymphocytes in vitro. 相似文献
32.
S Contreras M Rodríguez F al Momani C Sans S Esplugas 《Canadian Metallurgical Quarterly》2003,37(13):3164-3171
The effect of ozonation on the biodegradability of 100-ppm aqueous solutions of 2,4-dichlorophenol has been investigated. BOD at 5, 10 and 21 days, BOD/COD and BOD/TOC ratios and the average oxidation state are presented. Biodegradability measured as BOD5/COD ratio was increased from 0 of the original solution to 0.25 at the moment of removing all the initial compound (corresponding to an ozone dose of 0.12 g L-1, 0.48 for BOD21/COD ratio). To test the effect of this pre-treatment, the biological oxidation of these pre-ozonated solutions was performed in two semi-continuous stirred tank reactors, one with non-acclimated sludge and one with acclimated-to-phenol sludge. The study showed that the TOC content of the pre-treated solution could be removed up to 68% by an aerobic biological treatment as well as co-digested with municipal wastewater (TOC removal up to 82%), with similar operating retention times to a municipal wastewater plant (12-24 h). Kinetic studies based on Monod model have also been carried out. Pseudo-first-order kinetic constants were found to be in the range of 0.5-0.8 L g TVSS-1 h-1. 相似文献
33.
34.
A Fernandez Rodríguez M Arrabal Martín JF Domínguez Molinero JL Mijan Ortiz J Sánchez Tamayo A Zuluaga Gómez 《Canadian Metallurgical Quarterly》1995,19(8):620-626
A prospective study was conducted on 374 patients with urinary lithiasis, aiming to analyze the participation of oxalate in the lithogenesis and composition of the calcium oxalate calculi, alone or associated to other factors. METHODOLOGY: Metabolic urinary study of the patient and analysis of calculi with infrared spectrography and optical microscopy. RESULTS: 26.3% patients had hyperoxaluria and 77.5% of the calculi contain calcium oxalate; these are 167 cases of calcium oxalate, 110 of oxalate and calcium phosphate and 13 cases of mixed calcium oxalate and uric acid lithiasis. 43.4% patients with pure monohydrate calcium oxalate calculi have hypercalciuria, 22.6% hyperoxaluria and 19% hyperuricosuria. Dihydrated calcium oxalate calculi are related to high hypercalciuria in 65% cases and to significant hyperoxaluria in 35% cases. 45% patients present a single lithogenic factor, either hypercalciuria (49.6%), hyperoxaluria (20.6%), hyperuricosuria (13.74%), hypocitraturia (9%), urinary infection (1.5%), A.T.R. (2.25%) or acid oliguria (3%). 相似文献
35.
OE Santana Rodríguez ML Malé Gil JF HernándezSantana JM Limi?ana Ca?al AM Martín Sánchez 《Canadian Metallurgical Quarterly》1998,14(6):555-561
Von Hippel-Lindau disease (VHL) is an autosomal dominant tumour syndrome caused by germline mutations of the VHL tumour suppressor gene located on chromosome 3p25-26. In VHL tumours may occur in 14 different target organs, including the eye. Retinal angiomas are considered the first manifestation of VHL disease in 43% of cases, and the cumulative probability of developing a retinal angioma in one or both eyes rises during each decade of life, reaching 80% for patients over 80 years old. Since 1976 patients with VHL at the University Hospital of Utrecht and their at-risk relatives have been screened periodically by a multidisciplinary team. Long-term follow-up ophthalmological data were analysed with special attention to natural course and results of treatment. In addition, we looked for a genotype-phenotype correlation. Retinal angiomas were found in all families. In one large family with a missense mutation (V170D) of the VHL gene, in which the complete spectrum of visceral- and central nervous system (CNS) features of VHL is present, macular, parapapillary, optic disc and ora serrata angiomas were also found. In general, however, a clear-cut genotype-phenotype correlation could not be found. Only early detection and treatment of peripheral retinal angiomas can be expected to decrease the percentage of patients with decreased visual acuity. Therefore, early detection and treatment of these tumours is of paramount importance. Ophthalmological screening of patients and persons at risk should start as early as possible. In patients with apparently sporadic retinal angiomas it is advisable to perform germline DNA analysis, since the risk of developing VHL is high, especially if the angiomas are bilateral, or unilateral and multifocal, if the patient is young, or if there is a family history suggestive of VHL. 相似文献
36.
MP Amador Rodríguez A Artal Cortés E Rebollar Torres A Idoipe Tomás P Palomo Palomo J Martínez Trufero 《Canadian Metallurgical Quarterly》1998,13(4):198-204
A B16 melanoma-specific CD8+ T cell line (AB1) was established from the spleen cells of C57BL/6 mice cured of B16 melanoma with interleukin (IL)-12 treatment. The AB1 line exclusively used T cell receptor Vbeta11. The AB1 cells exhibited a cytolytic activity against both syngeneic B16 melanoma and allogeneic P815 mastocytoma, whereas a cold inhibition assay revealed specificity of the AB1 cells against B16 melanoma. Their lostability to kill a class I loss variant of B16 melanoma was restored by the transfection of H-2Kb gene. In addition, their interferon (IFN)-gamma production was significantly suppressed by the addition of anti-H-2Kb monoclonal antibody, and RT-PCR analysis showed that the AB1 line expressed the mRNA encoding IFN-gamma, but not IL-4 or IL-10. The experiment using synthetic peptides of tyrosinase-related protein-2 (TRP-2) revealed that the AB1 cells could recognize TRP-2(181-188) peptide. Moreover, the AB1 cells showed an in vivo antitumor effect against established pulmonary metastases of B16 melanoma. Overall, these results indicate that the Tc1-type Vbeta11+ AB1 cells exert an antitumor activity against syngeneic B16 melanoma through recognition of TRP-2(181-188) peptide in an H-2Kb-restricted manner. 相似文献
37.
Rodríguez-de-Vera Jesús M. González-Carrillo Josefa García José M. Bernabé Gregorio 《The Journal of supercomputing》2021,77(9):10138-10151
The Journal of Supercomputing - Left ventricular non-compaction (LVNC) is a rare cardiomyopathy characterized by abnormal trabeculations in the left ventricle cavity. Although traditional computer... 相似文献
38.
Sergio Herrerı´a-Alonso Miguel Rodrı´guez-Pérez Manuel Fernández-Veiga Cándido López-Garcı´a 《Computer Networks》2012,56(10):2456-2467
The IEEE 802.3az standard provides a new low power mode that Ethernet network interfaces can use to save energy when there is no traffic to transmit. Simultaneously with the final standard approval, several algorithms were proposed to govern the physical interface state transition between the normal active mode and the new low power mode. In fact, the standard leaves this sleeping algorithm unspecified to spur competition among different vendors and achieve the greatest energy savings. In this paper, we try to bring some light to the most well known sleeping algorithms, providing mathematical models for the expected energy savings and the average packet delay inflicted on outgoing traffic. We will then use the models to derive optimum configuration parameters for them under given efficiency constraints. 相似文献
39.
M. Oyanarte G. Besga M. Rodríguez M. Domingo A. G. Sinclair 《Nutrient Cycling in Agroecosystems》1996,47(3):261-269
Dairy farming is the main agricultural activity of the Basque Country. A dairy farm is characterized as a system with soils and crops, forage, cattle and manure as main components, and in such a system, nutrient cycling is very important to maintain soil fertility and optimize forage production. To quantify nutrient transfers in the cycle, a simple system was developed and has been applied to seventeen farms to examine its ability to achieve a balanced P and K fertilization. These farms have provided data on inputs (fertilizer, feeds, concentrates), pasture and manure management, and outputs (milk production), and soil samples have been taken from farm pastures. Phosphorus and K in excreta and uneaten pasture is used with a relatively high efficiency as suggested by the relatively high efficiency of P and K utilization by the pasture that usually ranges from 70 to 90%. Concentrate feeding (3000 kg cow–1 yr–1) represents one of the main P and K inputs in Basque Country dairy farms, averaging 26 and 66 kg ha–1, respectively. Besides, release of K in the soil through slow liberation from non-exchangeable sites was estimated as 30 kg ha–1. Thus, a high efficiency in excreta recycling would diminish substantially P and K mineral fertilizer needs. Farm nutrient budgets appear to be a convenient tool for determining nutrient shortages and surpluses at farm level, and thus they are considered as a first step to support a better management of maintenance fertilization of permanent pastures. 相似文献
40.
The present work analyzes the effect of various factors on the hydration of pentasodium triphosphate. The experimental method
is based on application of the hydration test. Technical-quality products with different proportions of phase I and phase
II have been used. The variables studied are phase I/phase II ratio, initial temperature, particle size, stirring rate and
composition of the slurry (presence of hexahydrate crystals and water hardness). The results have been discussed according
to a kinetics model that includes a series of stages of a physical nature (dissolution of anhydrous salt and the crystallization
of the hexahydrate), as well as of a chemical nature (solvation of the ions in solution). Crystallization of the hexahydrate
may be the controlling stage in the process. 相似文献