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61.
Using ion exchange chromatography and an ATP-dependent actin precipitation step, we have isolated three myosin-I isozymes that, together, account for most of the K+EDTA-ATPase activity recovered from extracts of Dictyostelium. The two major myosin-I isozymes, present in approximately equal amounts, had apparent molecular masses of 125 kDa on SDS gels and have been identified by amino acid sequence analysis as the products of the Dictyostelium myosin-IB (DMIB) and myosin-ID (DMID) genes. DMIB, with a specific K+EDTA-ATPase activity 10-fold higher than DMID, was responsible for most of the activity in cell extracts. The third isozyme, present in low amounts, had an apparent molecular mass of 137 kDa on SDS gels and is too large to be the product of any of the known myosin-I genes. DMIB eluted from DE53 cellulose columns as two distinct peaks (II and III). Addition of the phosphatase inhibitor okadaic acid to the extraction buffer increased the fraction of DMIB recovered from growth phase cells in peak III from 35 to 70%. DMIB isolated from peak III, but not from peak II, displayed a significant level of actin-activated MgATPase activity. These results indicate that peak III represents a phosphorylated, actin-activatable form of DMIB. 相似文献
62.
63.
The hydration phase and pore structure formation in the blends of sulfoaluminate-belite cement with Portland cement 总被引:1,自引:0,他引:1
Sulfoaluminate-belite (SAB) cements are an attractive class of low-energy cements from the viewpoint of saving energy and releasing less CO2 into the atmosphere during their production. Their hydraulic activity, however, does not match that of the ordinary Portland cement (PC) and needs improvement before they can be used on their own. However, SAB cements when blended with PC have the potential to be used effectively in traditional applications as shown by this study. Mortars made with blends of SAB cements and PC, and a cement-to-sand ratio of 1:3 by weight and a water-to-cement ratio of 0.5, indicate a superior protection against corrosion of steel to those made with blends of PC and blast-furnace slag (BFSPC). The prepared mortars were stored at 20 °C for 90 days under either a 60% relative humidity (RH)-dry air, or 100% RH-wet air conditions. With further improvement in the SAB cement quality through better understanding of their characteristics, a genuine competition between SAB/PC and BFSPC can be expected in practice. 相似文献
64.
Frantz Rowe 《欧洲信息系统杂志》2006,15(3):244-248
65.
66.
67.
Barbara K. Reck Robert B. Gordon 《JOM Journal of the Minerals, Metals and Materials Society》2008,60(7):55-59
Nickel and chromium are essential ingredients in alloys increasingly important for energy-efficient, environmentally friendly
modern technology. Quantitative assessment of the flows of these metals through the world economy from resource extraction
to final disposal informs resource policy, energy planning, environmental science, and waste management. This article summarizes
the worldwide technological cycles of nickel and chromium in 2000. Stainless steel is the major use of these metals, but they
serve numerous other special needs, as in superalloys for high-temperature service, as plating materials, and in coinage.
Because they are used primarily in alloys, novel recycling issues arise as their use becomes more widespread.
“... the great New York and St. Louis double track, nickel plated railroad...”
— Norwalk, Ohio, Chronicle 10 March 1881 announces arrival of surveyors for the future Nickel Plate Railway
“Later [1911] I formed an alloy of Iron and Chromium, which showed remarkable resistance to rust and tarnish ... [It was]
rediscovered by an Englishman named Brearley, in 1914.”
—Elwood Haynes to Stephen F. Roberts, 17 January 1925 相似文献
68.
Shafeeque G. Ansari Mushtaq Ahmad Dar Young-Soon Kim Hyung-Kee Seo Gil-Sung Kim Rizwan Wahab Zubaida A. Ansari Jae-Myung Seo Hyung-Shik Shin 《Korean Journal of Chemical Engineering》2008,25(3):593-598
A comparative study for the nucleation of diamond was carried out using surface treatment like (i) surface scratching with
1 μm diamond paste and (ii) surface etching using chlorine plasma at different RF powers (50, 100 and 150 W). Atomic force
microscopic study shows variation in roughness from 31 nm to 110 nm. Scratching results in random scratches, whereas plasma
etches a surface uniformly. Scanning electron microscopic observations show well faceted crystallites with a predominance
of angular shaped grains corresponding to 〈100〉 and 〈110〉 crystallite surfaces for the scratched as well as plasma etched
substrate. Surface etching at 150 W plasma power results in a better growth in comparison with 50 and 100 W plasma powers.
Chlorine-radical is found responsible for the changes in the growth morphology. Raman spectroscopy shows a sharp peak at 1,332
cm−1 and a peak at ∼1,580 cm−1 for both samples. 相似文献
69.
We study the error probability performance of rotated lattice constellations in frequency-flat Nakagami-m block-fading channels. In particular, we use the sphere lower bound on the underlying infinite lattice as a performance benchmark. We show that the sphere lower bound has full diversity. We observe that optimally rotated lattices with largest known minimum product distance perform very close to the lower bound, while the ensemble of random rotations is shown to lack diversity and perform far from it. 相似文献
70.
Jinsheng Que Qing Wang Jianping Chen Bingfei Shi Qinghui Meng 《Bulletin of Engineering Geology and the Environment》2008,67(4):479-483
The paper describes some geotechnical properties of the soft soils in Guangzhou College City, China, which are difficult to
both sample and test. Laboratory and in situ tests were carried out to assess the physical and mechanical indices of the soils.
The data were statistically analyzed and linear regression undertaken such that equations could be developed by which the
geotechnical properties of the soft soils can be predicted. The statistical validity of the degree of correlation confirmed
that using these equations, the mechanical indices can be estimated from physical indices determined by routine testing.
相似文献