Identification of cysteine pairs within the amino-terminal 5% of apolipoprotein B essential for hepatic lipoprotein assembly and secretion

There is growing evidence that the amino-terminal globular domain of apolipoprotein B (apoB) is essential for lipoprotein particle formation in the hepatic endoplasmic reticulum. To identify the structural requirements for its function in lipoprotein assembly, cysteine (Cys) pairs required to form the seven disulfide bonds within the amino-terminal 21% of apoB were replaced in groups or individually by serine. Substitution of Cys pairs required for formation of disulfide bonds 1-3 or 4-7 (numbered from amino to carboxyl terminus) completely blocked the secretion of apoB28 in transfected HepG2 cells. To identify the specific disulfide bonds required for secretion, Cys pairs were mutated individually. Substitution of Cys pairs required for disulfide bonds 1, 3, 5, 6, or 7 had little or no impact on apoB28 secretion or buoyant density. In contrast, individual substitution of Cys pair 2 (amino acid residues 51 and 70) or 4 (218 and 234) severely inhibited apoB28 secretion and its capacity to undergo intracellular assembly with lipid. The same assembly and secretion defects were observed when these mutations were expressed as part of apoB50. These studies provide direct evidence that the ability of the internal lipophilic regions of apoB to engage in the recruitment and sequestration of lipid during translation is critically dependent upon a structural configuration contained within or affected by the amino-terminal 5% of the protein.     

Kinetics of two-step anionic polymerization

This article deals with the kinetics of two-step anionic polymerization by way of a non-steady state method. Several molecular parameters can be evaluated using the formulae developed. A bimodal molecular weight distribution function for the resulting polymer is derived from the set of kinetic differential equations, which is in agreement with the experimental data reported.     

心电地图仪中工频干扰的一种滤除方法

在心电地图仪中记录到的体表心电信号往往由于电磁的影响而引进工频干扰．基于体表心电信号中工频干扰的特点，作者提出了一种滤除工频干扰的方法，即就单独—胸导联信号通过提高频谱的分辨率来估计工频干扰的频率；基于最小均方误差准则来估计各导联信号中工频干扰的幅度和相位。文中还给出了该算法的流程图和滤波性能分析。模拟结果表明了该算法的有效性。应用该算法对心电地图仪中的工频干扰进行对消，取得了满意的结果。     

用于提高国产氩离子激光器相干长度的相干扩展器

本文报导了最近研制成功的用于提高国产氩离子激光器相干长度的相干扩展器。利用这种基于F-P理论的相干扩展器可以使国产氩离子激光器的相干长度由不足5cm一下提高到m的量级,功率损耗在25%～40%之间,有着很好的应用前景。     

Magnetization and rotation of MTG HTSC ring in magnetic field

The magnetization of a melt-texture growth (MTG) HTSC ring has been studied. It is shown that the magnetic field inside the ring is larger than the external field under a certain range of external magnetic fields. We have also investigated the magnetic field dependence of the response of a detective coil near a rotating superconducting ring. The responses of the MTG sample are different for different cooling methods.     

面向表面微加工工艺的微器件设计框架的研究

本文以微器件设计为对象，对微硅表面制造驱动的微器件并行设计方法学及相应的计算机辅助设计工具的开发逻辑进行了研究，提出了采用基于三层Web-ASP集成框架实现e-CAD原型工具的编程方案，其中，并行任务流管理与控制，微器件实例及设计资源库，微加工工艺规划及仿真，微器件设计评价等是该系统实现的关键要素，在此基础上将建立网上微器件设计中心，并通过应用服务机制，实现对微器件设计工具使用的商务化，以达到降低设计成本，加快MEMS产业化之目的。     

An experience of tunnelling in mudstone area in southwestern Taiwan

Many tunnels will be constructed in southwestern Taiwan in the upcoming decade to meet the huge demands of transportation, energy and water infrastructure projects. Mudstone strata cover more than a 1000 km² area, consisting mainly of massive mudstone or alternation of mudstone and sandy layer, exhibiting unfavorable geological and hydrological characteristics, making it extremely difficult for tunnelling. This paper presents the lessons learned from three tunnelling projects in the 1990s in a mudstone area. In addition to discussing the rock behavior in the vicinity of tunnel and its failure patterns, the monitoring data during tunnelling are presented as well. Finally, the effective method of design and construction are recommended for tunnelling in mudstone area.     

展成式精密电火花加工机床

研制了一种展成式精密电火花加工机床，它含有3个回转机构和4个直线移动机构，工件苡轴，工具电极苡轴和转台均可按需要回转，坐标工作台可作纵向和横向移动，工件头架可作轴向和竖向移动。可设置成多种复合运动方式和相应的结构布局，具有很丰富的加工功能，加工精度能达到微米级并实现电火花镜面加工。