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排序方式: 共有1814条查询结果,搜索用时 15 毫秒
11.
T Matsunaga F Hirayama Y Yonemura R Murray M Ogawa 《Canadian Metallurgical Quarterly》1998,92(3):901-907
The receptors for interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), and IL-5 share a common signaling subunit betac. However, in the mouse, there is an additional IL-3 signaling protein, betaIL-3, which is specific for IL-3. We have previously reported that IL-3 abrogates the lymphoid potentials of murine lymphohematopoietic progenitors and the reconstituting ability of hematopoietic stem cells. We used bone marrow cells from betac- and betaIL-3-knock-out mice to examine the relative contributions of the receptor proteins to the negative regulation by IL-3. First, we tested the effects of IL-3 on lymphohematopoietic progenitors by using lineage-negative (Lin-) marrow cells of 5-fluorouracil (5-FU)-treated mice in the two-step methylcellulose culture we reported previously. Addition of IL-3 to the combination of steel factor (SF, c-kit ligand) and IL-11 abrogated the B-lymphoid potential of the marrow cells of both types of knock-out mice as well as wild-type mice. Next, we investigated the effects of IL-3 on in vitro expansion of the hematopoietic stem cells. We cultured Lin-Sca-1-positive, c-kit-positive marrow cells from 5-FU-treated mice in suspension in the presence of SF and IL-11 with or without IL-3 for 7 days and tested the reconstituting ability of the cultured cells by transplanting the cells into lethally irradiated Ly-5 congenic mice together with "compromised" marrow cells. Presence of IL-3 in culture abrogated the reconstituting ability of the cells from both types of knock-out mice and the wild-type mice. In contrast, addition of GM-CSF to the suspension culture abrogated neither B-cell potential nor reconstituting abilities of the cultured cells of wild-type mice. These observations may have implications in the choice of cytokines for use in in vitro expansion of human hematopoietic stem cells and progenitors. 相似文献
12.
Hiroki Yamaguchi Tsuneo Hanawa Oto Yamamoto Yu Matsuda Yasuhiro Egami Tomohide Niimi 《Microfluidics and nanofluidics》2011,11(1):57-64
The tangential momentum accommodation coefficient (TMAC) was investigated experimentally from the mass flow rate through a
single microtube under the slip flow and the early part of the transition regime. The measurements were carried out by the
constant-volume method under the mean Knudsen number smaller than 0.3, which is based on the mean pressure of the inlet and
the outlet of the microtube, to apply the second-order slip boundary condition. To measure TMACs on various materials, quite
large microtube was employed, which require the reduction in leakage. TMAC was obtained from the slip coefficient determined
by the relation of the mass flow rate to the mean Knudsen number. The obtained mass flow rate was well explained by the theoretical
equation. TMACs of deactivated-fused silica with argon, nitrogen, and oxygen were measured, showing the tangential momentum
was not accommodated completely to the surface, and the values showed good agreement with previous studies. From the comparison
between microtubes with different inner diameter, it is showed that TMAC is determined mainly by gas species and surface material. 相似文献
13.
Yusuke Fukui Yosuke Honda Yasuhiro Yamauchi Michiko Okafuji Masahiro Sakai Mikihiko Nishitani Yusuke Takata 《Journal of the Society for Information Display》2010,18(12):1090-1094
Abstract— MgO thin film is currently used as a surface protective layer for dielectric materials because MgO has a high resistance during ion sputtering and exhibits effective secondary electron emission. The secondary‐electron‐emission coefficient γ of MgO is high for Ne ions; however, it is low for Xe ions. The Xe content of the discharge gas of PDPs needs to be raised in order to increase the luminous efficiency. Thus, the development of high‐γ materials replacing MgO is required. The discharge properties and chemical surface stability of SrO containing Zr (SrZrO) as the candidate high‐γ protective layer for noble PDPs have been characterized. SrZrO films have superior chemical stability, especially the resistance to carbonation because of the existence of a few adsorption sites due to their amorphous structure. The firing voltage is 60 V lower than that of MgO films for a discharge gas of Ne/Xe = 85/15 at 60 kPa. 相似文献
14.
重复控制系统中非周期扰动的抑制 总被引:3,自引:0,他引:3
为了改善重复控制系统中的扰动抑制性能,提出一种基于曲率模型的扰动估计新方法.该方法的主要特点是扰动的估计值直接与重复控制规则结合也不影响重复控制系统的稳定性.仿真结果显示该方法可以有效地抑制扰动. 相似文献
15.
Thermal buckling of cross-ply laminated composite and sandwich plates according to a global higher-order deformation theory 总被引:4,自引:0,他引:4
A two-dimensional global higher-order deformation theory is presented for thermal buckling of cross-ply laminated composite and sandwich plates. By using the method of power series expansion of continuous displacement components, a set of fundamental governing equations which can take into account the effects of both transverse shear and normal stresses is derived through the principle of virtual work. Several sets of truncated Mth-order approximate theories are applied to solve the eigenvalue problems of a simply supported multilayered plate. Modal transverse shear and normal stresses can be calculated by integrating the three-dimensional equations of equilibrium in the thickness direction, and satisfying the continuity conditions at the interface between layers and stress boundary conditions at the external surfaces. Numerical results are compared with those of the published three-dimensional layerwise theory in which both in-plane and normal displacements are assumed to be C0 continuous in the continuity conditions at the interface between layers. Effects of the difference of displacement continuity conditions between the three-dimensional layerwise theory and the global higher-order theory are clarified in thermal buckling problems of multilayered composite plates. 相似文献
16.
Noriaki Murakami Koji Arafune Tadanobu Koyama Yoshimi Momose Tetsuo Ozawa Yasunori Okano Sadik Dost Le. H. Dao Masashi Kumagawa Yasuhiro Hayakawa 《Microgravity science and technology》2005,16(1-4):79-83
The effect of gravity on dissolution of GaSb in InSb melt and growth of InGaSb was experimentally investigated. Experiments were carried out in a GaSb(seed)/InSb/GaSb(feed) sandwich system under an imposed temperature gradient. In the experiments, the GaSb feed crystal dissolved into the InSb melt to supply the required GaSb component for the growth of In0.1Ga0.9Sb crystal. Two parameters were considered: (1) the inclination angle (θ) of the sample for gravity as 0° and 53°, and (2) the sample diameter (D) as 9 mm and 5mm. When θ was 0°, the interface was almost flat, indicating that convection was axisymmetric and stable. Whereas the interface was distorted towards gravitational direction when θ was 53°, indicating that solutal convection was dominant. The decrease of growth temperature and sample diameter reduced the distortion of interface and the dissolution amount of GaSb feed. The homogeneous crystals were grown at the initial growth stage by supplying the GaSb component during growth. 相似文献
17.
In order to non-destructively evaluate changes in toughness of cast duplex stainless steel, which is frequently used in main coolant pipes of PWR type nuclear power plants, due to thermal aging, we tried to apply thermoelectric power (TEP) measurement. because TEP is sensitive to microstructural material changes, and to clarify the mechanism behind TEP changes due to thermal aging. As a result, TEP of cast duplex stainless steel increased with aging time, and good correlations were found between TEP and toughness. Concerning the mechanism, TEP of high and low Cr content alloys was higher than that of intermediate Cr content alloys. Because high and low Cr areas are created in the ferrite phase due to thermal aging. TEP of the entire material increased. Furthermore, when each Cr fluctuating area acted in parallel, the increase in TEP became larger. According to the Mott-Jones theory, TEP is largely related to the electron density of states at the Fermi level. The electron density of states of Fe–Cr–Ni alloys in the valence band was measured with X-ray photoelectron spectroscopy (XPS). As a result, there was a high correlation between the TEP calculated from the XPS spectrum and the measured TEP. Therefore, we showed experimentally that the electron density of states changed due to variations in Cr concentration, which also affect TEP. 相似文献
18.
Atsushi Kanamori Fumio Tanaka Masaki Ominami Yuji Nadatani Shusei Fukunaga Koji Otani Shuhei Hosomi Noriko Kamata Yasuaki Nagami Koichi Taira Yasuhiro Fujiwara 《International journal of molecular sciences》2022,23(15)
The effects of psychological stress on eosinophilic gastrointestinal disorders have not been elucidated. This study investigated the effects of psychological stress in a mouse model of eosinophilic enteritis (EoN). BALB/c mice were treated with ovalbumin (OVA) to create an EoN model and subjected to either water avoidance stress (WAS) or sham stress (SS). Microscopic inflammation, eosinophil and mast cell counts, mRNA expression, and protein levels of type 2 helper T cell (Th2) cytokines in the ileum were compared between groups. We evaluated ex vivo intestinal permeability using an Ussing chamber. A corticotropin-releasing hormone type 1 receptor (CRH-R1) antagonist was administered before WAS, and its effects were analyzed. WAS significantly increased diarrhea occurrence and, eosinophil and mast cell counts, and decreased the villus/crypt ratio compared to those in the SS group. The mRNA expression of CRH, interleukin IL-4, IL-5, IL-13, eotaxin-1, and mast cell tryptase β2 significantly increased, and the protein levels of IL-5, IL-13, and OVA-specific immunoglobulin E (IgE) also significantly increased in the WAS group. Moreover, WAS significantly increased the intestinal permeability. The CRH-R1 antagonist significantly inhibited all changes induced by WAS. Psychological stress exacerbated ileal inflammation via the CRH-mast cell axis in an EoN mouse model. 相似文献
19.
Takao Yasui Noritada Kaji Yukihiro Okamoto Manabu Tokeshi Yasuhiro Horiike Yoshinobu Baba 《Microfluidics and nanofluidics》2013,14(6):961-967
Recent developments of nanofabrication techniques have created a trend switching from randomly ordered polymeric matrices, such as gel, to highly ordered sieving nanostructures in the separation of biomolecules. These nanostructures have enormous potential for fast separation of biomolecules, while nanostructure-based separation techniques suffer from critical scaling problems; they are efficient in handling less than nanoliter amounts of sample fluids, but most biomolecule samples are available in a liquid volume that is over several microliter, leading to a reduction in sensitivity and resolution. In this study, we developed a nanopillar array chip integrated with an easy and rapid on-line stacking method and achieved fast DNA separation with high sensitivity and high resolution. The developed on-line stacking method is based on the balance of two forces driven by electric fields: electroosmotic flow (EOF) and electrophoresis. The EOF mobility from the microchannel to the nanopillar-channel is drastically decreased, while, on the other hand, electrophoresis has constant mobilities in the whole length of the channels. The on-line stacking was realized at the well-balanced position of the two forces, and the on-line stacking using the nanopillar array chip can also be achieved within 10 s by just applying electric voltages without any other special reagents and materials. After applying on-line stacking using the nanopillar array chip, the relative fluorescence intensity increased 1,000-fold, and the resolution was twice as good as that without on-line stacking. 相似文献
20.
Physical therapists are trained in manual examination techniques to test the impaired motor functions of patients. In this study, we have introduced a wearable robotic dummy joint to simulate disordered joint resistances or behaviors to support physical therapists in learning such techniques. We developed a discontinuous joint friction model based on a stick-slip phenomenon to simulate knee joint resistances caused by crepitus, a typical symptom accompanied by osteoarthritis. Practicing therapists participated in a reality-based evaluation test and specified acceptable parameter sets to adjust the simulated crepitus for the exoskeletal patient robot. The simulated crepitus and wearable dummy joint are expected to support the training of physical therapists. 相似文献