Liquid film behavior in annular two-phase flow under flow oscillation conditions

Experiments were carried out to investigate the effects of sinusoidal forced oscillation of the inlet flow rate on the time variations of local liquid film thickness and the frequencies of large wave’s passing in steam–water annular two-phase flows. The liquid film thickness oscillated with the same period as the inlet flow rate. The mean film thickness in the thin film regions decreased and approached to an asymptotic value with an increase in the oscillation period of the inlet flow rate. This result was consistent with the experimental results of the occurrence of liquid film dryout under flow oscillation conditions reported in the literature. It was hence considered that the axial liquid transport from the thick to thin film regions mitigates the reduction of the critical heat flux caused by the flow oscillation. It was also found that the wave frequency in the thin film region increased with a decrease in the oscillation period. This observation suggested that the disturbance waves contribute to the enhancements of the liquid transport and consequently the critical heat flux associated with the liquid film dryout under flow oscillation conditions.     

Calculation of displacement cross-sections for structural materials in accelerators using PHITS event generator and its applications to radiation damage

The displacement cross-sections, implemented in the particle and heavy ion transport code system (PHITS), have been calculated to estimate the radiation damage in structural materials used at accelerator facilities. The event generator in PHITS was used to calculate displacement cross-sections for 14 elemental targets and two practical alloys irradiated with neutrons at energies from 10^?10 MeV to 3 GeV, and protons and deuterons at energies from several keV to 3 GeV. These displacement cross-sections were used to estimate the displacement per atom (DPA) in the target assembly at accelerator driven system (ADS) Target Test Facility (TEF-T) using 400-MeV protons, and in the Type 316 stainless steel target using 40-MeV deuterons. For the target assembly at TEF-T using 400-MeV protons, the DPA value for the proton component was approximately twice higher than that for the neutron component. For the Type 316 target using 40-MeV deuterons, radiation damage took place near the surface of Type 316 because of the Coulomb scattering effect between the incident deuterons and the material.     

Multistage Stack with Multiple Pore Radii Applying the Temperature Gradient to a Thermoacoustic Engine

Conventional thermoacoustic engines have a stack pore radius that is almost constant in the axial direction. Hence, a thermoacoustic engine is expected to improve the energy conversion efficiency using a multistage stack with multiple pore radii. The stack comprises several bundles of numerous narrow tubes with specified pore radii. The optimum pore radius of the stack is determined by the oscillation frequency and the temperature in the stack. Consequently, the suitable pore radius changes in the axial direction, because the temperature gradient exists along the stack axis. Therefore, a multistage stack with multiple pore radii is introduced, which achieves a desired optimum pore radius everywhere in the stack. The energy conversion efficiency of the multistage stack, which was studied experimentally for a straight-tube type thermoacoustic engine, was compared with that of a conventional single-stage stack. In these experiments, the improvement of the energy conversion efficiency was confirmed. A numerical method with the transmittance matrix to include the effect of a multistage stack was used, and good agreement between experimental and numerical results was obtained. The results make a future possibilities for stack design intended to higher thermoacoustic engine efficiency expect.     

Fabrication of transparent TiO2 film with high adhesion by using self-assembly methods: Application to super-hydrophilic film

A transparent and super-hydrophilic TiO₂ film with high adhesion was prepared by simple self-assembly methods from aqueous solution at low temperature. The excellent adherence of TiO₂ films was accomplished by introducing a buffer layer with sulfonate-modified surfaces and nanoasperity. Moreover, the structure and morphology of the films were successfully controlled by deposition temperature and the pH of precursor solution. By optimizing the several parameters of solution as well as the surface functionality of the substrate, the nano-structured TiO₂ film with high adhesion showed a water contact angle of below 5° and the relative transmittance to slide glass of over 90%. The fabricated TiO₂ film deposited under the optimized condition is not removed from substrate after several Scotch tape (STT) tests and immersing into several kinds of solvent.     

Direct evidence for covalent functionalization of carbon nanohorns by high-resolution electron microscopy imaging of C60 conjugated onto their skeleton

Carbon nanohorns (CNHs) functionalized with aryl units, possessing ethylene glycol chains terminated to amine groups, were condensed with modified C₆₀ carrying a free carboxylic acid group. Direct evidence for the covalent functionalization of CNHs was given by high-resolution transmission electron microscopy (HR-TEM) imaging of C₆₀ present in that CNH hybrid material. In addition, it was found that C₆₀ remained unaffected even after prolonged exposure to the electron beam. Finally, monitoring the wave motion of the connecting ethylene glycol chains, through sequential HR-TEM images, further proved the stability of the covalent bond that links the two carbon nanostructures.     

Temperature dependence for anammox bacteria enriched from freshwater sediments

The anoxic ammonium oxidation (anammox) process has been regarded as an attractive alternative process to treat wastewater containing high ammonium concentrations. By the implementation of anammox process at moderately low temperatures (<25°C), the anammox process will be applied to more various industrial wastewater treatments. In this study, we established enrichment cultures of anammox bacteria from freshwater sediments by using an up-flow column reactor equipped with porous polyester nonwoven fabric at moderately low temperatures. Their nitrogen conversion rates reached 0.07-0.26kg-N/m(3)/d. Phylogenetic analysis based on 16S rRNA gene from enrichment cultures revealed the presence of various anammox bacteria affiliated with unknown anammox bacteria as well as known anammox candidates, i.e., Candidatus Kuenenia stuttgartiensis and Candidatus Brocadia fulgida, Candidatus Scalindua wagneri. Anammox bacterial populations were influenced by enrichment conditions, i.e., seed sediments and temperature.     

Metal‐Free Growth of Nanographene on Silicon Oxides for Transparent Conducting Applications

Conventional methods to prepare large‐area graphene for transparent conducting electrodes involve the wet etching of the metal catalyst and the transfer of the graphene film, which can degrade the film through the creation of wrinkles, cracks, or tears. The resulting films may also be obscured by residual metal impurities and polymer contaminants. Here, it is shown that direct growth of large‐area flat nanographene films on silica can be achieved at low temperature (400 °C) by chemical vapor deposition without the use of metal catalysts. Raman spectroscopy and TEM confirm the formation of a hexagonal atomic network of sp²‐bonded carbon with a domain size of about 3–5 nm. Further spectroscopic analysis reveals the formation of SiC between the nanographene and SiO₂, indicating that SiC acts as a catalyst. The optical transmittance of the graphene films is comparable with transferred CVD graphene grown on Cu foils. Despite the fact that the electrical conductivity is an order of magnitude lower than CVD graphene grown on metals, the sheet resistance remains 1–2 orders of magnitude better than well‐reduced graphene oxides.     

Effect of pressure at primary drying of freeze-drying mouse sperm reproduction ability and preservation potential

Freeze-dried spermatozoa are capable of participating in normal embryonic development after injection into oocytes and thus useful for the maintenance of genetic materials. We recently reported that long-term preservation of freeze-dried mouse spermatozoa by conventional methods requires temperatures lower than -80 degrees C. Successful permanent preservation of mouse spermatozoa at much higher temperatures requires thorough investigation of the freeze-drying procedure. Thus, we examined the relationship between the pressure at primary drying and the preservation potential of freeze-dried mouse spermatozoa. Three different primary drying pressures were applied to evaluate the effect of pressure on freeze-dried spermatozoa under varying storage conditions and the rate of development measured. The developmental rate of embryos to the blastocyst stage from intracytoplasmic sperm injection by freeze-dried spermatozoa at pressures of 0.04, 0.37, and 1.03 mbar without storage were 59% (337/576), 71% (132/187), and 33% (99/302) respectively. When stored at 4 degrees C for 6 months, the rate was 13% (48/367), 50% (73/145), and 36% (66/182) respectively. These results show that primary drying pressure is an influential factor in the long-term preservation of freeze-dried mouse spermatozoa.     

Severe ethanol stress induces assembly of stress granules in Saccharomyces cerevisiae

Stress granules (SGs) and processing bodies (P bodies) are cytoplasmic domains and play a role in the control of translation and mRNA turnover in mammalian cells subjected to environmental stress. Recent studies have revealed that SGs also form in the budding yeast Saccharomyces cerevisiae in response to glucose depletion and robust heat shock. However, information about the types of stress that cause budding yeast SGs is quite limited. Here we demonstrate that severe ethanol stress generates budding yeast SGs in a manner independent of the phosphorylation of eIF2α. The concentration that generated budding yeast SGs (>10%) was higher than that causing P bodies (>6%), and P bodies were assembled prior to SGs. As well as mammalian SGs, the assembly of budding yeast SGs under ethanol stress was blocked by cycloheximide. On the other hand, the budding yeast SGs caused by ethanol stress contained eIF3c but not eIF3a and eIF3b, although the eIF3 complex is a core constituent of mammalian SGs. Moreover, null mutants (pbp1Δ, pub1Δ and tif4632Δ) with a strong reduction in SG formation did not resume proliferation after the elimination of ethanol stress, indicating that the formation of budding yeast SGs might play a role in sufficient recovery from ethanol stress.