Relationship between J-integral and averaged strain-energy density for U-notches in the case of large control volume under Mode I loading

The main purpose of this technical note is to present a relationship between J-integral and averaged strain-energy density () in U-notches under Mode I loading for brittle or quasi-brittle materials. In this work, control volume includes the rectilinear edge of the notch in addition to semi-circular arc of the notch root. A dimensionless function (f) between J and has been presented in this paper. Finite element analysis has been used for verification. It is found that this relationship is identical for tension or bending loading.     

Kinetic speciation of Co(II), Ni(II), Cu(II), and Zn(II) in model solutions and freshwaters: lability and the d electron configuration

The kinetic speciation of Co(II), Ni(II), Cu(II), and Zn(II) in model solutions of a well-characterized fulvic acid (Laurentian fulvic acid), freshwater samples from the Rideau River (Ottawa, Ontario), and freshwater samples from the Sudbury (Ontario) area were investigated by the competing ligand exchange method using Chelex 100 as the competing ligand and by inductively coupled plasma-mass spectrometry to measure the dissociation kinetics. The metal species were quantitatively characterized by the rate coefficient for the first-order dissociation of metal complex to free metal ion. This technique can be applied to almost all elements and represents an important advance in our ability to investigate the kinetic availability of metal species in the freshwater environment. The order of the lability of the metal complexes, Co(II) > Ni(II) > Cu(II) < Zn(II), follows the reverse order of the ligand field stabilization energy with the exception of Cu(II); the behavior of Cu(II) is also due to the Jahn-Teller effect, which shortens the equatorial bonds and lengthens the axial bonds of a tetragonally distorted Cu(II)-L6 complex. This study has demonstrated a relationship between the lability of metal-DOM complexes of the 3d transition metals in freshwaters and their d electron configuration. This is the first time that the importance of the d electron configuration on the lability of metal complexes in the freshwater environment has been demonstrated. The slow complexation kinetics of both Ni(II) and Cu(II) suggestthatthe usual equilibrium assumption for freshwaters may be invalid.     

Aging, cardiac proenkephalin mRNA and enkephalin peptides in the Fisher 344 rat

Cardiac proenkephalin (PENK) mRNA, methionine-enkephalin (ME) and leucine-enkephalin (LE) were determined from 2 days of age through senescence in Fisher 344 rats. Tissues were collected at 2 days, 2 weeks, 1, 2, 3, 7, 19, and either 22 or 27 months of age. Hearts were dissected, extracted and assayed for ME and LE by radioimmunoassay (RIA) or for PENK mRNA by Northern blot analysis with a cDNA probe. Relative left ventricular (LV) PENK mRNA was low in 2 day animals and increased slowly between 2 weeks and 3 months of age. LV PENK mRNA then rose five to six-fold between 3 and 27 months of age. LV ME measurements were high in neonatal animals, declined to a nadir during development and then rose again as the animals matured and advanced in age. The pattern for right ventricular (RV) ME was similar. Atrial ME, also high at 2 days, declined thereafter and remained low. LE measurements in LV, RV and the atria followed patterns similar to those described for ME. To evaluate for peptides contributed by cardiac nerves, 3, 7 and 22-month-old animals were acutely sympathectomized for 24 h with 6-hydroxydopamine. No decline in LV ME and LE was observed in the 6-hydroxydopamine treated animals. These data suggest several conclusions regarding myocardial enkephalinergic systems: (a) tissue enkephalin and PENK mRNA increase with advancing age, (b) tissue enkephalins may not strictly correlate with the relative abundance of PENK mRNA, and (c) most myocardial enkephalins are non-adrenergic in origin. The age-associated patterns in both PENK mRNA, ME and LE suggest that physiological, maturational or behavioral events between 3 and 7 months of age initiate the up-regulation and subsequent expansion of cardiac enkephalinergic systems.     

Analysis of damping characteristics of squeeze films under dynamic (cyclic) loading

This paper presents an analytical study of the damping characteristics of circular discs subjected to dynamic loads. Assuming a circular hydrostatic thrust bearing describing different geometrical configurations and surface texture irregularities (on both the macro- and micro-scales) and subjected to sinusoidal harmonic excitations, an analytical formulation to identify the oil film thickness variation as a function of damping loads is proposed. The Reynolds equation in the discretized form has been solved numerically using the tdma (Thomas Algorithm). Such a direct technique has proved to be reliable in solving similar problems and minimizing computational time compared with conventional iterative procedures. Compared to the performance of a flat and smooth circular disc, results indicated that the micro- and macro-surface undulations play a major role in dictating the squeeze action damping characteristics     

Surgical removal of pulmonary metastasis: prospective study in 182 patients]

Dystrophic neurites are major components of neuritic (both immature and mature) senile plaques in Alzheimer disease. Previous studies have shown strong immunoreactivity for different neuropeptides, and chromogranin A, a protein associated with dense-core vesicles, in dystrophic neurites. In the present study, antibodies to synaptophysin, synapsin, Rab3a and synaptotagmin (synaptic vesicle proteins), and SNAP-25 (synaptosomal-associated protein of 25 kD) and syntaxin (presynaptic plasma membrane proteins) have been used to learn about the dystrophic neurite equipment of proteins that are necessary for the docking and fusion of synaptic vesicles, and then for exocytosis and neurotransmission. The present results have shown that, although most neuritic senile plaques have chromogranin A- and SNAP-25-immunoreactive dystrophic neurites, only a percentage of them contain synaptophysin, and a minority contain synaptotagmin and Rab3a. Dystrophic neurites do not contain synapsin and syntaxin. These results show that dystrophic neurites of senile plaques are defective in proteins that control exocytosis and neurotransmission.     

Absence of prognostic significance of bcl-2 immunopositivity in non-small cell lung cancer: analysis of 427 cases

The bcl-2 gene product inhibits apoptosis and is thought to participate in oncogenesis. Association of bcl-2 immunopositivity with improved prognosis of non-small cell lung cancers (NSCLC) is controversial. Although two studies have reported better survival in bcl-2-immunopositive NSCLCs, a third series has contradicted this finding. The authors studied a relatively larger case series involving 427 patients for whom detailed information on long-term follow-up was available to determine the prognostic significance of bcl-2 expression. The study included 252 adenocarcinomas (AC), 111 squamous cell carcinomas (SCC), and 64 large cell carcinomas (LC). After antigen retrieval, sections were immunostained using a monoclonal anti-bcl-2 antibody (1:60, Clone 124, Dako) and the avidin-biotin complex technique. Staining was scored as positive or negative and also on a semiquantitative scale as 0, low (<10%), moderate (10% to 75%), or extensive (>75%). Bcl-2 immunoreactivity was correlated with survival using the actuarial survival method, Kaplan-Meier method, and log-rank test and was not associated with statistically significant differences in survival for NSCLCs (P = .5537). Differences in survival remained insignificant even after NSCLCs were stratified for cell type, stage, or grade, singly or in combination. Therefore, using this method, bcl-2 immunopositivity does not appear to act as an independent prognostic indicator in NSCLCs.