Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.     

Excitonic photoluminescence in CuAlS2 powders

Copper aluminum disulfide (CuAlS₂) powders were synthesized in the evacuated ampoule. The luminescence properties of obtained powders were evaluated by photoluminescence (PL) at various temperatures. The visible emission peaks at 2.16 and 1.90 eV, and weak ultraviolet emission peak at 3.47 eV were obtained in the room temperature PL. The temperature dependence of the PL revealed that the ultraviolet emission at room temperature was considered to be free-exciton. Several emission peaks related to free-exciton, bound-exciton and phonons were observed in the high-resolution PL at 12 K, reflecting that the obtained powders had high crystallinity. Emission peaks at 3.459 and 3.300 eV for CuAlS₂ were observed for the first time. The former emission might be originated from the bound exciton and the latter from the donor–acceptor pair recombination.     

Effect of heparin on high glucose induced proliferation and expression of matrix metalloproteinases in normal human mesangial cells

Background The pathogenesis of diabetic nephropathy (DN) is a complex pathophysiological process.Its precise mechanism is not fully known. In recent years it has been recognized that synthesis of various extracelluar matrix (ECM) components may increase, and that degradation of ECM may decrease in DN. It was reported heparin could inhibit mesangial cells proliferation in vitro. The main aim of this study is to explore whether heparin inhibits proliferation of mesangial cells grown in high glucose concentration and to measure the effect of heparin on matrix metalloproteinases (MMPs) expression in mesangial cells. Methods The medium contained either low glucose (5 mmol/L) or high glucose (25 mmol/L). The concentrations of heparin in the culture medium were 0, 25, 50,100, 200 or 400 μg/mL. A metabolic (WST-1) assay was used to measure mesangial cell proliferation and Western blot analysis was used to measure MMPs expression of mesangial cells. Results Normal human mesangial cell (NHMC) proliferation was higher in high glucose (HG) medium than in low glucose (LG) medium. They showed a 1.93 fold expansion after 72 h in high glucose in contrast to a 1.63 fold expansion in low glucose. In the presence of heparin, mesangial cells proliferation was inhibited, which was more obvious at high glucose concentrations than at low glucose concentrations. In high glucose, with heparin concentration of 50, 100, 200 and 400 μg/mL, the mesangial cells showed a 0. 61 fold, 0.52 fold, 0.52 fold and 0.41 fold reductions in cell number compared to cells grown without heparin. In low glucose, only concentrations of 200 μg/mL and 400 μg/mL showed reduction in cell number, namely 0.54 fold and 0.45 fold, when compared to cells grown without heparin. In Western blot analysis,MMP1, MMP2, MMP3 and MMP9 was expressed by mesangial cells expressed in both high and low glucose concentrations, which was more prominent in high glucose medium. Incubation of heparin further increased expression of MMP1, MMP2, MMP3 and MMP9. Conclusions This study suggests that glucose can accelerate mesangial cell proliferation while heparin can reduce proliferation, being more obvious at high glucose concentrations. Higher glucose concentrations led to increased MMP expression, which may take part in the regulation of mesangial matrix synthesis and degradation. Addition of heparin resulted in a corresponding increase in MMP expression, most notably at high glucose concentrations, indicating a potentially renoprotective role in DN.     

Characterization of autoinducer 2 signal in Eikenella corrodens and its role in biofilm formation

Quorum sensing (QS) is a process by which bacteria communicate using secreted chemical signaling molecules called autoinducers (AIs). By this process, many bacterial species modulate the expression of a wide variety of physiological functions in response to changes in population density. In this study, the periodontal pathogen Eikenella corrodens was observed to secrete type 2 signaling molecules. An ortholog of luxS, the gene required for AI-2 synthesis in Vibrio harveyi, was isolated from the E. corrodens genome. A V. harveyi bioassay showed luxS functionality in E. corrodens and the ability of luxS to complement the luxS-negative phenotype of Escherichia coli DH5alpha. AI activity was detected in the supernatant, and the maximum expression of AI-2 was observed during the late exponential phase. To determine the potential role of luxS in the colonization processes, an E. corrodens luxS mutant was constructed and tested for its capacity to form an in vitro biofilm on a polystyrene surface. The biofilm forming efficiency of the luxS mutant was approximately 1.3-fold greater than that of the wild type. These data suggest that a LuxS-dependent signal plays a role in the biofilm formation by E. corrodens.     

Effects of operating and design parameters on PEFC cold start

During startup from subzero temperatures the water produced in a polymer electrolyte fuel cell (PEFC) forms ice/frost in the cathode catalyst layer (CL), blocking the oxygen transport and causing cell shutdown once all CL pores are plugged with ice. This paper describes an experimental study on the effects of operating and design parameters on PEFC cold-start capability. The amount of total product water in mg cm⁻² during startup is used as an index to quantify the cold-start capability. The newly developed isothermal cold-start protocol is used to explore the basic physics of cold start, and the effects of purge methods prior to cold start, startup temperature and current density, and the membrane thickness are shown. The experimental data also confirm the current density effect predicted earlier by a multiphase model of PEFC cold start.     

Route-Enabling Graph Orientation Problems

Given an undirected and edge-weighted graph G together with a set of ordered vertex-pairs, called st-pairs, we consider two problems of finding an orientation of all edges in G: min-sum orientation is to minimize the sum of the shortest directed distances between all st-pairs; and min-max orientation is to minimize the maximum shortest directed distance among all st-pairs. Note that these shortest directed paths for st-pairs are not necessarily edge-disjoint. In this paper, we first show that both problems are strongly NP-hard for planar graphs even if all edge-weights are identical, and that both problems can be solved in polynomial time for cycles. We then consider the problems restricted to cacti, which form a graph class that contains trees and cycles but is a subclass of planar graphs. Then, min-sum orientation is solvable in polynomial time, whereas min-max orientation remains NP-hard even for two st-pairs. However, based on LP-relaxation, we present a polynomial-time 2-approximation algorithm for min-max orientation. Finally, we give a fully polynomial-time approximation scheme (FPTAS) for min-max orientation on cacti if the number of st-pairs is a fixed constant.     

Effect of self-representation of interaction history by the robot on perceptions of mind and positive relationship: a case study on a home-use robot

A question in research is concerning the most important factors for increasing the motivation of a human to continue interacting with an interactional robot. Previous studies have argued that the perceptions of mind and interactability of the robot by the interacting human are essential factors. However, ways of improving such perceptions have not sufficiently investigated. In this study, we focused on the case of a home-use interactional robot and investigated ways to improve such perceptions. We proposed the perceived interaction history of the robot to be another essential factor and hypothesized that adding this factor can promote the perceptions of mind and positive relationship. We conducted a human–robot interaction experiment with 32 human subjects, and the experimental results verified our hypothesis.     

A bilevel decomposition algorithm for simultaneous production scheduling and conflict-free routing for automated guided vehicles

We address a bilevel decomposition algorithm for solving the simultaneous scheduling and conflict-free routing problems for automated guided vehicles. The overall objective is to minimize the total weighted tardiness of the set of jobs related to these tasks. A mixed integer formulation is decomposed into two levels: the upper level master problem of task assignment and scheduling; and the lower level routing subproblem. The master problem is solved by using Lagrangian relaxation and a lower bound is obtained. Either the solution turns out to be feasible for the lower level or a feasible solution for the problem is constructed, and an upper bound is obtained. If the convergence is not satisfied, cuts are generated to exclude previous feasible solutions before solving the master problem again. Two types of cuts are proposed to reduce the duality gap. The effectiveness of the proposed method is investigated from computational experiments.