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91.
p-Nitrophenol (PNP) -degrading bacteria were isolated from a reactor using a mineral salt medium containing a low and high PNP concentration. We isolated two bacterial species, Pseudomonas sp. YTK17 and Rhodococcus opacus YTK32, that utilize PNP as their sole source of carbon and energy. These strains exhibited differences in PNP degradation activity in relation to PNP concentration. Strain YTK17 showed a high level of degradation following pre-exposure to a low PNP concentration, whereas strain YTK32 required a relatively high PNP concentration for degradation to occur. These results indicated that phylogenetically and physiologically different types of PNP-degrading bacteria coexisted in a reactor. 相似文献
92.
Abhrajit Ghosh Yitzchak M. Gottlieb Aditya Naidu Akshay Vashist Alexander Poylisher Ayumu Kubota Yukiko Sawaya Akira Yamada 《中国通信》2013,10(3):56-66
In this paper, we present Real-Time Flow Filter (RTFF) —a system that adopts a middle ground between coarse-grained volume anomaly detection and deep packet in-spection. RTFF was designed with the goal of scaling to high volume data feeds that are common in large Tier-1 ISP networks and providing rich, timely information on ob-served attacks. It is a software solution that is designed to run on off-the-shelf hardware platforms and incorporates a scalable data processing architecture along with lightweight analysis algorithms that make it suitable for deployment in large networks. RTFF also makes use of state of the art machine learning algorithms to construct attack models that can be used to detect as well as predict attacks. 相似文献
93.
Fumihiro Muto Tomoyuki Kakigi Naohiro Mitani Akio Matsuura Kazuki Fujii Yukiko Sato Jingye Li Masakazu Washio 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2007,265(1):162-167
Polymer electrolyte fuel cell (PEFC) membranes based on thin film of crosslinked perfluorinated polymer-alloys (RX-FA) have been fabricated by soft electron beam (soft-EB) grafting with styrene monomers using soft-EB irradiation under nitrogen atmosphere at room temperature (RT). The characteristic properties of styrene-grafted materials (GRX-FA) and sulfonated materials (SRX-FA) have been measured by differential scanning calorimetry (DSC) and FT-IR spectroscopy, ionic conductivity and so on. The glass transition temperatures (dry state) of all obtained SRX-FA were about 105 ± 1 °C, which are higher than Nafion®. The ion exchange capacities of SRX-FA have been achieved about 3.3 meq/g (dry). The ionic conductivity of obtained SRX-FA has showed about 0.17 S/cm at 60 °C with relative humidity (RH) of ∼95%. The ionic conductivities of the obtained SRX-FA were higher than that of conventional perfluoro-sulfonic acid membranes (PFSA). Fabricated membrane electrode assemblies (MEAs) based on the obtained SRX-FA have shown encouraging performance in the PEFC, compared with the conventional PFSA. The power density of obtained MEAs based on the SRX-FA was about 330-340 mW/cm2 under 500 mA/cm2 at 60 °C operation. Moreover, the maximum power densities of obtained MEAs based on the SRX-FA shows about 630 mW/cm2 at 60 °C. On the other hand, the power density at 500 mA/cm2 and maximum power density of MEA based on Nafion®112 were about 320 and 590 mW/cm2 at 60 °C. Thus, the power density of the obtained SRX-FA was higher than that of conventional PFSA. 相似文献
94.
Jin-Hua Baoyindugurong;Jia-Le Hou;Ya-Nan Ren;Ya-Wen Li;Mailisi Heshuote; Hugejiletu;Naoki Ohara;Yukiko Naito;Kenjiro Tatematsu; 《Food Science & Nutrition》2024,12(6):4443-4458
The aim of this study is to combine flaxseed oil (FO), rich in α-linolenic acid (ALA), with Sunite sheep tail fat (STF) through a lipase-catalyzed transesterification reaction, in order to produce an edible oil with a fatty acid ratio suitable for human needs. Initially, the optimal conditions for esterification were determined using the Box–Behnken design, with the measurement criterion being the content of ALA at the sn-2 position. The results indicated that the highest content of sn-2 ALA was obtained under the conditions of using 6.8 wt% Lipozyme®RMIM as the catalyst, a reaction temperature of 57°C, a reaction time of 3.3 h, and a substrate mass ratio of 5.6:4.4 for STF and FO. This led to the rapid breaking and recombining of molecular bonds, resulting in the interesterified fat (IF) with the highest content of ALA at the sn-2 position. Comparing STF and FO, IF exhibited excellent fatty acid composition and content. Furthermore, IF had a lower melting point and crystallization temperature compared to STF, and its solid fat content decreased with increasing temperature, completely melting at temperatures above 30°C. Thus, IF is a synthesized fat with excellent properties from both animal and vegetable sources. 相似文献
95.
Takashi Koida Jiro Nishinaga Yuko Ueno Hirofumi Higuchi Hideki Takahashi Masayuki Iioka Yukiko Kamikawa Hajime Shibata Shigeru Niki 《Progress in Photovoltaics: Research and Applications》2019,27(6):491-500
Polycrystalline W and H codoped In2O3 (In2O3:W,H) transparent conducting oxide (TCO) layers with high electron mobility were found to enhance both the short‐circuit current density (Jsc) and fill factor (FF) of Cu(In,Ga)Se2 (CIGS) modules with glass/Mo/CIGS/CdS/ZnO/TCO structures. An In2O3:W,H TCO layer was formed by reactive plasma deposition without substrate heating, followed by postannealing at 150°C. A phase transition from amorphous to polycrystalline was confirmed during postannealing, and a layer with micrometer‐scale lateral grain sizes evenly covered the rough ZnO surface generated by the naturally textured CIGS. The crystallized In2O3:W,H film showed high electron mobility (apprixmately 80 cm2 V−1 s−1) at a carrier density of approximately 2 to 3 × 1020 cm−3. Therefore, the In2O3:W,H layer achieved lower sheet resistance and lower free carrier absorption than a ZnO:Al layer, which is commonly used in CIGS modules. The CIGS mini‐module employing the In2O3:W,H layer with a designated area of 3.792 cm2 exhibited a higher efficiency (η) of 20.93% (Voc = 3.081 V [translated value per subcell: 0.770 V], short‐circuit current = 33.21 mA [Jsc = 35.03 mA cm−2], FF = 0.775) with improved Jsc and FF, when compared with a mini‐module employing a ZnO:Al layer. The results indicate that polycrystalline In2O3‐based TCOs with high electron mobility are suitable for CIGS solar cells and modules. 相似文献
96.
Chihiro Goto Motoharu Hirano Dr. Katsuhiko Hayashi Prof. Yutaka Kikuchi Dr. Yukiko Hara-Kudo Dr. Takashi Misawa Dr. Yosuke Demizu 《ChemMedChem》2019,14(22):1911-1916
Magainin 2 ( Mag 2 ), which is isolated from the skin of frogs, is a representative antimicrobial peptide (AMP), exerts its antimicrobial activity via microbial membrane disruption. It has been reported that both the amphipathicity and helical structure of Mag 2 play an important role in its antimicrobial activity. In this study, we revealed that the sequence of 17 amino acid residues in Mag 2 (peptide 7 ) is required to exert sufficient activity. We also designed a set of Mag 2 derivatives, based on enhancement of helicity and/or amphipathicity, by incorporation of α,α-disubstituted amino acid residues into the Mag 2 fragment, and evaluated their preferred secondary structures and their antimicrobial activities against both Gram-positive and Gram-negative bacteria. As a result, peptide 11 formed a stable helical structure in solution, and possessed potent antimicrobial activities against both Gram-positive and Gram-negative bacteria without significant cytotoxicity. 相似文献
97.
98.
Noriyuki Hatano Masaki Matsubara Hiroka Suzuki Yukiko Muraki Katsuhiko Muraki 《International journal of molecular sciences》2021,22(12)
Intracellular free zinc ([Zn2+]i) is mobilized in neuronal and non-neuronal cells under physiological and/or pathophysiological conditions; therefore, [Zn2+]i is a component of cellular signal transduction in biological systems. Although several transporters and ion channels that carry Zn2+ have been identified, proteins that are involved in Zn2+ supply into cells and their expression are poorly understood, particularly under inflammatory conditions. Here, we show that the expression of Zn2+ transporters ZIP8 and ZIP14 is increased via the activation of hypoxia-induced factor 1α (HIF-1α) in inflammation, leading to [Zn2+]i accumulation, which intrinsically activates transient receptor potential ankyrin 1 (TRPA1) channel and elevates basal [Zn2+]i. In human fibroblast-like synoviocytes (FLSs), treatment with inflammatory mediators, such as tumor necrosis factor-α (TNF-α) and interleukin-1α (IL-1α), evoked TRPA1-dependent intrinsic Ca2+ oscillations. Assays with fluorescent Zn2+ indicators revealed that the basal [Zn2+]i concentration was significantly higher in TRPA1-expressing HEK cells and inflammatory FLSs. Moreover, TRPA1 activation induced an elevation of [Zn2+]i level in the presence of 1 μM Zn2+ in inflammatory FLSs. Among the 17 out of 24 known Zn2+ transporters, FLSs that were treated with TNF-α and IL-1α exhibited a higher expression of ZIP8 and ZIP14. Their expression levels were augmented by transfection with an active component of nuclear factor-κB P65 and HIF-1α expression vectors, and they could be abolished by pretreatment with the HIF-1α inhibitor echinomycin (Echi). The functional expression of ZIP8 and ZIP14 in HEK cells significantly increased the basal [Zn2+]i level. Taken together, Zn2+ carrier proteins, TRPA1, ZIP8, and ZIP14, induced under HIF-1α mediated inflammation can synergistically change [Zn2+]i in inflammatory FLSs. 相似文献
99.
Hiroshi Mitoma Shinji Kakei Kazuhiko Yamaguchi Mario Manto 《International journal of molecular sciences》2021,22(9)
The cerebellum is endowed with the capacity for compensation and restoration after pathological injury, a property known as cerebellar reserve. Such capacity is attributed to two unique morphological and physiological features of the cerebellum. First, mossy fibers that convey peripheral and central information run mediolaterally over a wide area of the cerebellum, resulting in the innervation of multiple microzones, commonly known as cerebellar functional units. Thus, a single microzone receives redundant information that can be used in pathological conditions. Secondly, the circuitry is characterized by a co-operative interplay among various forms of synaptic plasticity. Recent progress in understanding the mechanisms of redundant information and synaptic plasticity has allowed outlining therapeutic strategies potentiating these neural substrates to enhance the cerebellar reserve, taking advantage of the unique physiological properties of the cerebellum which appears as a modular and potentially reconfiguring brain structure. 相似文献
100.
Taisuke Tomonaga Hiroto Izumi Yukiko Yoshiura Chinatsu Nishida Kazuhiro Yatera Yasuo Morimoto 《International journal of molecular sciences》2021,22(9)
This work studies the relationship between lung inflammation caused by nanomaterials and surfactant protein D (SP-D) kinetics and investigates whether SP-D can be a biomarker of the pulmonary toxicity of nanomaterials. Nanomaterials of nickel oxide and cerium dioxide were classified as having high toxicity, nanomaterials of two types of titanium dioxides and zinc oxide were classified as having low toxicity, and rat biological samples obtained from 3 days to 6 months after intratracheal instillation of those nanomaterials and micron-particles of crystalline silica were used. There were different tendencies of increase between the high- and low-toxicity materials in the concentration of SP-D in bronchoalveolar-lavage fluid (BALF) and serum and in the expression of the SP-D gene in the lung tissue. An analysis of the receiver operating characteristics for the toxicity of the nanomaterials by SP-D in BALF and serum showed a high accuracy of discrimination from 1 week to 3 or 6 months after exposure. These data suggest that the differences in the expression of SP-D in BALF and serum depended on the level of lung inflammation caused by the nanomaterials and that SP-D can be biomarkers for evaluating the pulmonary toxicity of nanomaterials. 相似文献