Separation of non-denatured proteins using semi-crosslinked polyacrylamide capillary gel electrophoresis

This work presents an approach to build a high-performance, low-viscous and replaceable separation matrix, semi-crosslinked polyacrylamide （semi-CPA） capillary gel electrophoresis. Non- denatured basic proteins, such as lysozyme, cytochrome C, ribonuclease A and trypsin were separa- ted. The impacts of monomer and cross-linker concentrations on protein separation were studied, and the ability of dynamic capillary inner wall coating was demonstrated. The UV absorption interfer- ence by semi-CPA gel matrix was successfully overcome by a partial filling technique, which results in sensitivity 20 times higher than other protein separation method. The excellent separation ability, reproducibility and dynamic coating ability made semi-CPA an ideal separation media in both capillar- y electrophoresis and microfluidic chip separation scheme.     

DA、5-HT及其代谢产物HPLC-ECD检测方法的研究

采用高效液相色谱结合库仑阵列多电极检测器体系（HPLC—ECD）建立了一套操作简便、高效、快速测定多巴胺（DA）、3，4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）、5-羟色胺（5-HT）以及5-羟吲哚乙酸（5-HIAA）的方法，并探究了检测的灵敏性，浓度和响应因子之间的线性关系，系统的稳定性等。DA、DOPAC、HVA、5-HT及5-HIAA的相关系数分别为0．9964、0．9999、1、0．9989、0．9999；HVA和5-HIAA的检测限都低于0．5ng／mL，DA、DOPAC及5-HT的检测限均低于0．1ng／mL，各种物质的方法回收率为85．6％-107．4％。     

压力驱动的毛细管等电聚焦分离牛血清白蛋白和血红蛋白

蛋白质的聚焦过程完成后,实现了压力驱动毛细管内完成聚焦的聚焦区带迁移,进行聚焦区带的检测.实验证明,在较低流速下不会破环聚焦区带,得到了理想的分离结果,方法可行,且操作简单.     

New Cell Viability Measurement of SH-SY5Y Cells in the PD Research-Alamarblue~(TM)

The traditional MTT assay requires destructive analyses and is not convenient in continuous monitory of cell viability. However, a new cell model was developed in this research, by using the oxidation-reduction (redox) indicator alamarblue? instead of the MTT assay. The alamarblue?does no harm to cells and provide a much more safe and convenient methods of measurement. Firstly, cell apoptosis was induced by different concentration of 6-OHDA, then the cell viability was tested by the alamarblue?at a serial of time points. Finally, the optimism cells density, 6-OHDA concentration and testing time point were gained to set a wonderful SH-SY5Y cell model in our research. And when it is applied in the study of neuroprotection effects of NAC, GSH and Catalase, the new model reveals undeniable advantages.     

论建筑企业中的激励

目前我国建筑企业面临着如何吸引人才、留住人才以及激励人才为企业目标而努力工作的激励问题。本文从激励的机理和机制两个方面对激励理论展开了阐述,并结合建筑企业的激励实践进行了讨论,总结了建筑企业的激励机制选择问题,最后讨论了企业激励存在的问题。本文的研究有助于我们系统地了解激励理论,指导建筑企业的激励实践。     

双对置柴油机排气歧管CFD仿真与优化设计

双对置发动机具有扫气质量高、低油耗、低排放及自身平衡性好等优点,其中排气系统设计的优劣直接关系到双对置发动机的动力性和经济性。通过CFD软件对发动机排气歧管内的三维流场进行了数值模拟,评估了各支管流量的均匀性,流动是否发生分离,从而判断排气歧管设计是否合理,并对不合理结构进行了优化设计,提高了发动机性能。     

南水北调项目群集成管理研究

以南水北调工程为研究对象,提出了考虑层次维度的南水北调项目群总体结构。在项目群及项目群管理一般理论的基础上,运用集成管理的思想和理念,结合工程实际情况,对南水北调项目群集成管理问题做了深入的研究和分析,建立了南水北调项目群集成化管理的概念模型,阐述了项目群信息、目标、要素、过程、组织集成的内涵及5个方面的相互关系。     

Synthesis of ino Acid Derived β-Cyclodextrins Used in Chiral Separation by Capillary Electrophoresis

Six new kinds of ino acid derived β-cyclodextrins were synthesized to improve their water solubility and chiral separation properties. They are heptakis{2,6-di-O-[3-L-(1-isopropyl carboxyl methyl ino)-2-hydroxy propyl]}-β-cyclodextrin (i.e. L-Val-β-CD), heptakis{2,6-di-O-[3-L-(1-benzyl carboxyl methyl ino)-2-hydroxy propyl]}-β-cyclodextrin (i.e. L-Phe-β-CD), heptakis{2,6-di-O-[3-(D, L-1-benzyl carboxyl methyl ino)-2-hydroxy propyl]}-β-cyclodextrin (i.e. D,L-Phe-β-CD), heptakis{2,6-di-O-[3-(L-1-hydroxymethyl carboxyl methyl ino)-2-hydroxy propyl]}-β-cyclodextrin (i.e. L-Ser-β-CD), heptakis{2,6-di-O-[3-(L-1-carboxylmethyl carboxyl methyl ino)- 2-hydroxy propyl]}-β-cyclodextrin (i.e. L-Asp-β-CD), heptakis{2,6-di-O-[3-(L-2-carboxyl tetrethylene ino)-2-hydroxy propyl]}-β-cyclodextrin (i.e. L-Pro-β-CD). Their chemical structures were certified using FTIR and 1H NMR. Except for L-Phe-β-CD and D,L-Phe-β-CD, that are in soluble in water, the other ino acid derived β-CDs all have good water solubility. D,L-tyrosine and promethazine were baselinely separated by L-Val-β-CD in capillary electrophoresis.     

一种空间细胞培养与在线观测一体化装置的研制

空间生物学的蓬勃发展,给相关实验仪器的发展带来了新的机遇和挑战,而细胞模型是空间生命科学应用最广泛的模型之一,为此世界航天强国开发了一系列细胞培养装置。本文报道了一种空间细胞培养与在线观测一体化装置的研制与相关性能测试结果。本装置由专用培养单元、通用培养单元和通用操作单元组成,培养基由注射泵驱动,其余液体试剂由蠕动泵驱动。专用培养单元的主要功能是开展细胞间接共培养实验;通用培养单元是通用细胞培养平台;通用操作单元可驱动多种试剂进入通用培养单元中完成固定、裂解、染色或消化等操作。装置内通用培养模块和专用培养模块均配有可调焦可见光显微成像系统,可对培养的细胞进行实时观测并记录细胞的形态。最后,我们测试本装置的温度控制和显微成像分辨率,并使用本装置对U-87 MG细胞和SF767细胞进行培养、显微成像和染色,结果表明本装置满足哺乳动物细胞培养以及在线观测的要求。本装置可广泛应用于空间环境下细胞的培养,同时也可用于其他需要自动化细胞培养与在线观测的实验中。     

一种适用于小型毛细管电泳装置的紧凑型荧光检测器的研制

空间生命科学的蓬勃发展伴随着对高性能生化分析装置的强烈需求。国家重大科学仪器设备开发专项课题涉及针对小分子分离分析的毛细管电泳分析仪的装置研发,预期在封装上执行中国载人航天空间站Ⅱ型机柜插箱标准,在功能上全自动完成样品前处理、高效分离、高灵敏度检测等常规操作。为满足尺寸明显小于商用仪器的高灵敏度检测的应用需求,本研究开发了一种基于共聚焦光路的紧凑型荧光检测器,将该检测器初步应用于异硫氰酸荧光素的检测,获得了良好的检测效果和重现性,为最终构建满足实际空间环境应用的航天载荷奠定重要基础。