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22.
In this paper we introduce a framework to represent robot task plans based on Petri nets. Our approach enables modelling a robot task, analysing its qualitative and quantitative properties and using the Petri net representation for actual plan execution. The overall model is obtained from the composition of simple models, leading to a modular approach. Analysis is applied to a closed loop between the robot controller and the environment Petri net models. We focus here on the quantitative properties, captured by stochastic Petri net models. Furthermore, we introduce a method to identify the environment and action layer parameters of the stochastic Petri net models from real data, improving the significance of the model. The framework building blocks and a single-robot task model are detailed. Results of a case study with simulated soccer robots show the ability of the framework to provide a systematic modelling tool, and of determining, through well-known analysis methods for stochastic Petri nets, relevant properties of the task plan applied to a particular environment. 相似文献
23.
Rare earth (RE) ions have spectroscopic characteristics to emit light in narrow lines, which makes RE complexes with organic ligands candidates for full color OLED (Organic Light Emitting Diode) applications. In particular, β-diketone rare earth (RE3+) complexes show high fluorescence emission efficiency due to the high absorption coefficient of the β-diketone and energy transfer to the central ion. In this work, the fabrication and the electroluminescent properties of devices containing a double and triple-layer OLED using a new β-diketone complex, [Eu(bmdm)3(tppo)2], as transporting and emitting layers are compared and discussed. The double and triple-layer devices based on this complex present the following configurations respectively: device 1: ITO/TPD (40 nm)/[Eu(bmdm)3(tppo)2] (40 nm)/Al (150 nm); device 2: ITO/TPD (40 nm)/[Eu(bmdm)3(tppo)2] (40 nm)/Alq3 (20 nm)/Al (150 nm) and device 3: ITO/TPD (40 nm)/bmdm-ligand (40 nm)/Al (150 nm), were TPD is (N,N′-diphenyl-N,N′-bis(3-methylphenyl)-1,1-biphenil-4,4-diamine) and bmdm is butyl methoxy-dibenzoyl-methane. All the films were deposited by thermal evaporation carried out in a high vacuum system. These devices exhibit high intensity photo- (PL) and electro-luminescent (EL) emission. Electroluminescence spectra show emission from Eu3+ ions attributed to the 5D0 to 7FJ (J = 0, 1, 2, 3 and 4) transitions with the hypersensitive 5D0 → 7F2 transition (around 612 nm) as the most prominent one. Moreover, a transition from 5D1 to 7F1 is also observed around 538 nm. The OLED light emission was almost linear with the current density. The EL CIE chromaticity coordinates (X = 0.66 and Y = 0.33) show the dominant wavelength, λd = 609 nm, and the color gamut achieved by this device is 0.99 in the CIE color space. 相似文献
24.
Wide parabolic wells can be created by properly controlling the Al content during the growth of successive Ga1–xAlxAs thin layers. Under a tilted magnetic field these systems present interesting transport properties, which are associated to their composition dependent g-factor. We present an exact solution for the eigenstates of an electron gas inside such a quantum well. We calculate the renormalized cyclotron frequencies as functions of the angle of tilt, as well as the density of states, and the Fermi level. We discuss the conditions for the existence of spin-polarized charge. 相似文献
25.
Gabriel Alves Bonaf Jssica Silva dos Santos Jussara Vaz Ziegler Fernando Augusto Lima Marson Thalita Rocha Manoela Marques Ortega 《International journal of molecular sciences》2022,23(13)
Glycyrrhizic acid (GA), a natural compound isolated from licorice (Glycyrrhiza glabra), has exhibited anti-inflammatory and anti-tumor effects in vitro. Dipotassium glycyrrhizinate (DPG), a dipotassium salt of GA, also has shown an anti-tumor effect on glioblastoma cell lines, U87MG and T98G. The study investigated the DPG effects in the melanoma cell line (SK-MEL-28). MTT assay demonstrated that the viability of the cells was significantly decreased in a time- and dose-dependent manner after DPG (IC50 = 36 mM; 24 h). DNA fragmentation suggested that DPG (IC50) induced cellular apoptosis, which was confirmed by a significant number of TUNEL-positive cells (p-value = 0.048) and by PARP-1 [0.55 vs. 1.02 arbitrary units (AUs), p-value = 0.001], BAX (1.91 vs. 1.05 AUs, p-value = 0.09), and BCL-2 (0.51 vs. 1.07 AUs, p-value = 0.0018) mRNA compared to control cells. The proliferation and wound-healing assays showed an anti-proliferative effect on DPG-IC50-treated cells, also indicating an inhibitory effect on cell migration (p-values < 0.001). Moreover, it was observed that DPG promoted a 100% reduction in melanospheres formation (p-value = 0.008). Our previous microRNAs (miRs) global analysis has revealed that DPG might increase miR-4443 and miR-3620 expression levels. Thus, qPCR showed that after DPG treatment, SK-MEL-28 cells presented significantly high miR-4443 (1.77 vs. 1.04 AUs, p-value = 0.02) and miR-3620 (2.30 vs. 1.00 AUs, p-value = 0.01) expression compared to control cells, which are predicted to target the NF-kB, CD209 and TNC genes, respectively. Both genes are responsible for cell attachment and migration, and qPCR revealed significantly decreased CD209 (1.01 vs. 0.54 AUs, p-value = 0.018) and TNC (1.00 vs. 0.31 AUs, p-value = 2.38 × 10−6) mRNA expression levels after DPG compared to untreated cells. Furthermore, the migration of SK-MEL-28 cells stimulated by 12-O-tetradecanoylphorbol-13-acetate (TPA) was attenuated by adding DPG by wound-healing assay (48 h: p-value = 0.004; 72 h: p-value = 7.0 × 10−4). In addition, the MMP-9 expression level was inhibited by DPG in melanoma cells stimulated by TPA and compared to TPA-treated cells (3.56 vs. 0.99 AUs, p-value = 0.0016) after 24 h of treatment. Our results suggested that DPG has an apoptotic, anti-proliferative, and anti-migratory effect on SK-MEL-28 cells. DPG was also able to inhibit cancer stem-like cells that may cause cerebral tumor formation. 相似文献
26.
Claudio Bernardazzi Morgana Teixeira Lima Castelo-Branco Beatriz Pêgo Beatriz Elias Ribeiro Siane Lopes Bittencourt Rosas Patrícia Teixeira Santana Joo Carlos Machado Camille Leal Fabiano Thompson Robson Coutinho-Silva Heitor Siffert Pereira de Souza 《International journal of molecular sciences》2022,23(9)
27.
Juliano B. Lima Ricardo M. Campello de Souza 《Applicable Algebra in Engineering, Communication and Computing》2011,22(5-6):393-411
In this paper, the finite field trigonometric transforms (FFTT) are introduced. The FFTT family includes eight types of cosine transforms and eight types of sine transforms, which are developed from concepts related to the finite field trigonometry. New lemmas are presented and some properties of the FFTT are discussed. 相似文献
28.
Tests of Random Walk: A Comparison of Bootstrap Approaches 总被引:1,自引:1,他引:0
This paper compares different versions of the multiple variance ratio test based on bootstrap techniques for the construction
of empirical distributions. It also analyzes the crucial issue of selecting optimal block sizes when block bootstrap procedures
are used. The comparison of the different approaches using Monte Carlo simulations leads to the conclusion that methodologies
using block bootstrap methods present better performance for the construction of empirical distributions of the variance ratio
test. Moreover, the results are highly sensitive to methods employed to test the null hypothesis of random walk. 相似文献
29.
Diane Cleydes Baía da Silva Leonardo Oliveira Bittencourt Daiane Claydes Baia-da-Silva Victoria Santos Chemelo Luciana Eir-Quirino Priscila Cunha Nascimento Mrcia Cristina Freitas Silva Marco Aurelio M. Freire Walace Gomes-Leal Maria Elena Crespo-Lopez Rafael Rodrigues Lima 《International journal of molecular sciences》2022,23(7)
Methylmercury (MeHg) is one of the most dangerous toxic pollutants spread throughout the earth. Chronic MeHg intoxication by contaminated food ingestion is the most common threat to human health, including impairment to the developing fetus. The present study aims at investigating the effects of maternal exposure to MeHg during gestation and lactation on the spinal cord of offspring. Pregnant rats received oral doses of MeHg (40 μg/kg/day) over a period of 42 days (21 gestation and 21 lactation). Control animals received the vehicle only. Total mercury concentration was measured in blood samples from offspring collected at the 41st postnatal day. Counting of motor neurons and immunoreactivity for myelin basic protein (MBP) were assessed in the spinal cords in both control and MeHg-intoxicated animals. Our results showed that MeHg promoted an increase in blood Hg levels. In addition, it caused a reduction in the number of spinal cord motor neurons as well as decreased MBP immunoreactivity in the cervical, thoracic and lumbar segments. Our present findings suggest that MeHg intoxication during rat pregnancy and lactation is associated with a pattern of motor neuron degeneration and downregulation of myelin basic protein in different segments of a developing spinal cord. Further studies are needed to establish the effect of MeHg intoxication in both young and adult rats. 相似文献
30.
Nicoletta Braidotti Maria Augusta do R. B. F. Lima Michele Zanetti Alessandro Rubert Catalin Ciubotaru Marco Lazzarino Orfeo Sbaizero Dan Cojoc 《International journal of molecular sciences》2022,23(8)
The connection between cytoskeleton alterations and diseases is well known and has stimulated research on cell mechanics, aiming to develop reliable biomarkers. In this study, we present results on rheological, adhesion, and morphological properties of primary rat cardiac fibroblasts, the cytoskeleton of which was altered by treatment with cytochalasin D (Cyt-D) and nocodazole (Noc), respectively. We used two complementary techniques: quartz crystal microbalance (QCM) and digital holographic microscopy (DHM). Qualitative data on cell viscoelasticity and adhesion changes at the cell–substrate near-interface layer were obtained with QCM, while DHM allowed the measurement of morphological changes due to the cytoskeletal alterations. A rapid effect of Cyt-D was observed, leading to a reduction in cell viscosity, loss of adhesion, and cell rounding, often followed by detachment from the surface. Noc treatment, instead, induced slower but continuous variations in the rheological behavior for four hours of treatment. The higher vibrational energy dissipation reflected the cell’s ability to maintain a stable attachment to the substrate, while a cytoskeletal rearrangement occurs. In fact, along with the complete disaggregation of microtubules at prolonged drug exposure, a compensatory effect of actin polymerization emerged, with increased stress fiber formation. 相似文献