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71.
Solubility and liquid-liquid equilibrium data have been obtained for the ternary system Water-Phosproric Acid-Di-isopropyl Ether at 25 and 40°C. This system presents a three-phase1 region at bcth temperatures. Data for the tie lines end the solubility curve in the zone above the three-phase region at 25°C have been obtained by analyzing the phcsphcric acid and the di-isopropyl ether composition in the conjugated phases. Di-isoprcpyl ether i"as analyzed by NMR, using acetic acid as internal standard. It was impossible to apply conventional cloud point method to this zone due to the presence of an isooptic region. 相似文献
72.
ANA ROCÍO ROLDÁN PALOMO PEDRO MARTÍN ALEJANDRO REBOLLEDO NICOLÁS ENRIQUE LUIS E. FLORES VERÓNICA MILESI 《Biocell》2012,36(2):73-81
After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca2+-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. In presence of 2-APB the magnitude of a second paired contraction by serotonin or histamine was significantly less than a first one, likely because 2-APB inhibited store refilling by capacitative Ca2+ entry. 2-APB inhibition of sarcoplasmic reticulum Ca2+ release was excluded because this blocker did not affect serotonin force development in a Ca2+-free solution. The PCR technique showed the presence of mRNAs for STIM proteins (1 and 2), for Orai proteins (1, 2 and 3) and for TRPC channels (subtypes 1, 3, 4 and 6) in the smooth muscle of the human umbilical artery. Hence, this artery presents a capacitative contractile response triggered by stimulation with physiological vasoconstrictors and expresses mRNAs for proteins and channels previously identified as SOCs. 相似文献
73.
Red Kidney and Pinto dry beans were processed into a ready-to-eat snack food by several methods. The dry beans were soaked in water overnight or germinated under controlled conditions at 22°C for 4 days prior to processing. The germination process removed most of the raffinose and stachyose in the beans as revealed by HPLC analysis. Protease inhibitor activity in the beans was greatly reduced by cooking the rehydrated beans in tomato sauce or by frying the beans in hydrogenated vegetable oil at 180°C for 6 min. The in vitro protein digestibility of the beans was improved as evidenced by the decrease in trypsin and chymotrypsin inhibitors in the cooked and fried products. 相似文献
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The effects of temperature and several chemicals on gelation time and strength of gels formed by heating (pH 8) 5% solutions of trypsin hydrolyzed sunflower proteins were studied by dynamic rheological methods. The storage modulus reached a maximum at 80°C. Ca2Cl (and NaCl at > 0.2M) accelerated gelation and weakened the gel. NaCOCH3Na2SO4 and NaSCN decreased the storage modulus. Urea decreased gelstrength and at high concentrations slowed gelation. Time for gelation diminished and gel strength increased with increasing mercaptoethanol concentration up to 0.1M. Propylene glycol at 5–20% concentrations accelerated gelation and at 5% also increased gel strength. Trypsin hydrolyzed sunflower proteins could be useful in products requiring strong gels at high temperatures. 相似文献
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DANIELA M SACCARO ADNAN Y TAMIME ANA LÚCIA O P S PILLEGGI MARICÊ N OLIVEIRA 《International Journal of Dairy Technology》2009,62(3):397-404
This study investigated the viability of probiotic ( Lactobacillus acidophilus LA5, Lactobacillus rhamnosus LBA and Bifidobacterium animalis subsp . lactis BL-04) in milk fermented with Lactobacillus delbrueckii subsp . bulgaricus LB340 and Streptococcus thermophilus TAO (yoghurt – Y). Each probiotic strain was grown separately in co-culture with Y and in blends of different combinations. Blends affected fermentation time(s), pH and firmness during storage at 4°C. The product made with Y plus B. animalis subsp . lactis and L. rhamnosus had counts of viable cells at the end of shelf life that met the minimum required to achieve probiotic effect. However, L. acidophilus and L. delbrueckii subsp . bulgaricus were inhibited. 相似文献
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JOSÉ C ANDRADE KELLY ASCENÇÃO PATRICIA GULLÓN SILVINO M S HENRIQUES JORGE M S PINTO TERESA A P ROCHA‐SANTOS ANA CRISTINA FREITAS ANA MARIA GOMES 《International Journal of Dairy Technology》2012,65(4):467-481
Conjugated linoleic acid (CLA) is a group of isomers of linoleic acid (LA) with several potential health benefits which justifies the efforts to obtain foods with enhanced CLA contents. A possible way to obtain CLA‐enriched products is via fermentation as several micro‐organisms, many of which are employed in the manufacture of foods, are known to convert free LA into CLA. However, due to several constraints, the use of CLA‐producing bacteria still remains challenging and continuous efforts are required. This review summarises the current knowledge on microbial CLA production by food‐grade micro‐organisms, its technical issues and limitations, potential applications and bioactivities. 相似文献