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31.
Partitioning of bovine serum albumin (BSA) in an aqueous double countercurrent distribution (DCCD) using a three-phase system was studied and compared with countercurrent distribution using a two-phase system. The two-phase system was composed of Dextran and Polyethylene glycol (PEG) solulions, and the three-phase system consisted of Dextran, Ficoll, and PEG solutions. In the double countercurrent distribution experiment, BSA was partitioned from the stationary bottom phase into the moving middle and top phases. Two peaks resulted from these transfers. A mathematical model that predicts peak positions and shapes based on the partitioning behavior of the solute is provided. Based on model predictions, the DCCD method has potential extraction selectivity and recovery advantages over the traditional CCD method. 相似文献
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ROBERTA CERIANI ANTONIO J.A. MEIRELLES RAFIQUL GANI 《Journal of food process engineering》2010,33(S1):208-225
As the need for healthier fats and oils (natural vitamin and trans fat contents) and interest in biofuels are growing, many changes in the world's vegetable oil market are driving the oil industry to developing new technologies and recycling traditional ones. Computational simulation is widely used in the chemical and petrochemical industries as a tool for optimization and design of (new) processes, but that is not the case for the edible oil industry. Thin-film deodorizers are novel equipment developed for steam deacidification of vegetable oils, and no work on the simulation of this type of equipment could be found in the open literature. This paper tries to fill this gap by presenting results from the study of the effect of processing variables, such as temperature, pressure and percentage of stripping steam, in the final quality of product (deacidified palm oil) in terms of final oil acidity, the tocopherol content and neutral oil loss. The simulation results have been evaluated by using the response surface methodology. The model generated by the statistical analysis for tocopherol retention has been validated by matching its results with industrial data published in the open literature.
This work is a continuation of our previous works ( Ceriani and Meirelles 2004a, 2006 ; Ceriani et al. 2008 ), dealing with the simulation of continuous deodorization and/or steam deacidification for a variety of vegetable oils using stage-wised columns, and analyzing both the countercurrent and the cross-flow patterns. In this work, we have studied thin-film deodorizers, which are novel equipment developed for steam deacidification of vegetable oils. Here, we highlight issues related to final oil product quality and the corresponding process variables. 相似文献
PRACTICAL APPLICATIONS
This work is a continuation of our previous works ( Ceriani and Meirelles 2004a, 2006 ; Ceriani et al. 2008 ), dealing with the simulation of continuous deodorization and/or steam deacidification for a variety of vegetable oils using stage-wised columns, and analyzing both the countercurrent and the cross-flow patterns. In this work, we have studied thin-film deodorizers, which are novel equipment developed for steam deacidification of vegetable oils. Here, we highlight issues related to final oil product quality and the corresponding process variables. 相似文献
34.
ANTONIO TORNAMBÉ 《International journal of systems science》2013,44(9):1475-1489
State estimation for non-linear dynamic systems is discussed. A high-gain injection from the output variables is used to attenuate to any desired degree, the effect of the non-linear terms on the estimation errors, which can be made arbitrarily small, from a particular observable form. Some Liapunov arguments are used to study the stability properties of the resulting error dynamics. It is shown that any completely observable multi-input multi-output non-linear system, not necessarily linear in the input variables, can be transformed, by means of a change of coordinates depending on the input variables, in such an observable form. In particular, the solvability of partial differential equations is not needed for the design of this transformation. 相似文献
35.
L.Z. PINO R.B. SOLARI S. SIQUIER L. ANTONIO ESTÉVEZ M.M. YÉPEZ A.E. SÁEZ 《Chemical Engineering Communications》2013,200(1):367-382
This work presents experimental data on gas holdup in slurry bubble columns with a foaming liquid. The effects of solids concentration, solid particle size, superficial phase velocities and column dimensions on the gas holdup are analyzed. At low superficial gas velocities (less than 4cm/s), for which the liquid does not foam, the presence of solids with small particle size does not affect the gas holdup whereas solids with large particle size induce foam formation and thus their presence increases the gas holdup. In the foaming regime, an increase of solids concentration decreases the gas holdup. The operating mode has a strong effect on the gas holdup: the semi-batch operating mode (stagnant liquid-solid suspension) increases the ability of the liquid to foam with respect to the continuous mode. Regarding the effect of column dimensions, the results presented show that the height of the bubble column does not affect at an appreciable extent the gas holdup in the range 6 < LID < 12. At high gas velocities (greater than 6 cm/s) the gas holdups obtained in a 30 cm-internal diameter column are the same as those measured in a 10 cm-internal diameter column. 相似文献
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MARÍA BEGOÑA SAN MARTÍN TERESA FERNÁNDEZ-GARCÍA ANTONIO ROMERO ANTONIO LÓPEZ 《Journal of Food Processing and Preservation》2001,25(5):309-321
Hazelnut quality was studied after storage in selected modified atmosphere conditions. Hazelnuts of the Negret cultivar collected in Tarragona, Spain, were used in the experiments. The shelled and unshelled hazelnuts were stored with different oxygen concentrations (1%, 5%, 10% and 20% O2 ) at two different temperatures (7C and 25C). The quality of the hazelnuts during storage was monitored by determining the peroxide value, acid value, K232 and K270 indices, percentage of unsaturated fatty acids and sensory analysis of the hazelnuts. It was observed that after one year storage period, none of the storage modes had given rise to significant rancidity. It was also confirmed that the shell protected the hazelnut from oxidative deterioration. Nevertheless, storage in atmospheres with oxygen levels lower than 10%, autoxidation was significantly reduced and the low temperature delayed lipid rancidity. 相似文献
38.
ELEONIDAS MOURA LIMA MARIANA FERREIRA LEAL MARÍLIA DE ARRUDA CARDOSO SMITH ROMMEL RODRÍGUEZ BURBANO PAULO PIMENTEL DE ASSUMPÇÃO MARIA JOSE BELLO JUAN ANTONIO REY FRANCINALDO FERREIRA DE LIMA CACILDA CASARTELLI 《Biocell》2008,32(3):237-243
Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric cancer samples. MLH1 and PMS2 methylation were associated with neoplastic samples compared to nonneoplastic ones. PMS2 methylation was associated with diffuse- and intestinal-type cancer compared with normal controls. Intestinal-type cancer showed significant association with MLH1 methylation. Diffuse-type cancer was significantly associated with MSH2 methylation. Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that methylation is associated with gastric carcinogenesis. Methylation of mismatch repair genes was associated with gastric carcinogenesis and may be a helpful tool for diagnosis, prognosis and therapies. However, MSH6 does not seem to be regulated by methylation in our samples. 相似文献
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