Contribution of the C-terminal amino acid to the stability of Bacillus subtilis neutral protease

The role of the C-terminal Leu300 in maintaining thermal stabilityof the neutral protease of Bacillus subtilis was investigated.From model building studies based on the three dimensional structureof thermolysin, the neutral protease of B.thermoproteolyticus,it was conduded that this residue is located in a hydrophobicpocket composed of residues located in the C-terminal and themiddle domain. To test the hypothesis that Leu300, by contributingto a stabilizing interaction between these domains, is importantfor enzyme stability, several neutral protease mutants wereconstructed and characterized. The thermostability of the enzymewas lowered by deleting Leu300 or by replacing this residueby a smaller (Ala), a polar (Asn) or a sterically unfavourable(He) amino acid. Thermostabiity was increased upon replacingLeu300 by Phe. These results are in agreement with model-buildingstudies. The effects on thermostability observed after mutatingthe corresponding Val318 in the thermostable neutral proteaseof B.stearothermophilus were less pronounced.     

Designing for self-organisation in sociotechnical systems: resilience engineering,cognitive work analysis,and the diagram of work organisation possibilities

In designing sociotechnical systems, accounting for the phenomenon of self-organisation is critical. Empirical studies show that workers in these systems adapt not just their individual behaviours, but also their collective structures to deal with complex work environments. The concept of self-organisation can explain how such adaptations can be achieved spontaneously, continuously, and relatively seamlessly, and why this phenomenon is important for dealing with instability, uncertainty, and unpredictability in the task demands. However, existing design approaches such as resilience engineering and cognitive work analysis are limited in their capacity to design for self-organisation. This paper demonstrates that the diagram of work organisation possibilities, a recent addition to cognitive work analysis, provides a sound theoretical basis for designing for self-organisation. That is, it shows how essential components of the diagram are aligned with the concept of self-organisation and are well-grounded in empirical observations of adaptation in a variety of sociotechnical systems, specifically emergency management, military, and healthcare systems. Consequently, designs based on this diagram should have the potential to facilitate the emergence of new spatial, temporal, and functional organisational structures from the flexible actions of individual, interacting actors, thereby enhancing a system’s capacity for dealing with a dynamic, ambiguous work environment. Future research should focus on validating these ideas and demonstrating their value in industrial settings.

     

一种基于并联机构的航天器姿态模拟装置的设计开发

航天器发射升空前进行地面测试和试验是航天器研制过程中的重要环节。文中针对一种基于并联机构的姿态模拟装置的设计开发,从位置分析、速度分析和虚拟样机的建造和仿真分析等方面对3UPS-PU并联机构进行研究,完成姿态模拟装置的设计,为航天器装配、试验和测试设备的设计提供一种新的思路。     

辽宁省水能资源开发利用管理现状分析

《辽宁省水能资源开发利用管理条例》自2010年实施以来,辽宁省水能资源开发规划、项目建设管理、安全生产监管、绿色小水电建设、安全生产标准化电站建设的各项规章制度已逐步建立并完善,水能资源管理能力得到显著提升,辽宁省水能资源开发利用管理逐步走上法制化、规范化轨道。本文对此加以介绍。     

Marketing Activities to Support ‘Moderately Novel’ Product Innovation: Insights from the Chemical Industry

Scholars often follow a contingency approach to study which marketing activities are suitable for a particular type of product innovation project, thereby making a distinction between incremental and radical innovation only. ‘Moderately novel’ projects, which have intermediate levels of newness, have therefore not been given due attention. This paper focuses on market intelligence generation and the creation of cross‐functional linkages as marketing activities that are important in the context of moderately novel product innovation. In addition, the organizational position of the marketers involved in these activities is dealt with. Based on the analysis of four successful projects in the chemical industry, we argue, firstly, that moderately novel innovation projects have their own particular sets of marketing practices and, secondly, that differences exist between projects aiming at a new market segment and projects in which novelty is not related to market segment but to other market dimensions. These differences are especially salient in early project phases. These findings are pertinent to research on the role of marketers in product innovation, and to the study of organizational ambidexterity.     

ILU预处理Newton-Krylov方法的潮流计算

由于电力系统修正方程组具有高维、稀疏的特点,本文提出将预处理Krylov子空间方法应用于潮流修正方程组的求解,形成预处理Newton-Krylov的潮流计算方法。结合ILU预处理方法,比较了最常用的3类Newton-Krylov方法求解潮流方程的计算效果。通过对 IEEE30、IEEE118、IEEE300 和3个Poland大规模电力系统进行潮流计算,结果表明：3类Newton-Krylov方法是电力系统潮流计算的有效方法,呈现出良好的收敛特性和计算效率。     

The Cost of Environmental Constraints in Traffic Networks: Assessing the Loss of Optimality

Increasing numbers of hard environmental constraints are being imposed in urban traffic networks by authorities in an attempt to mitigate pollution caused by traffic. However, it is not trivial for authorities to assess the cost of imposing such hard environmental constraints. This leads to difficulties when setting the constraining values as well as implementing effective control measures. For that reason, quantifying the cost of imposing hard environmental constraints for a certain network becomes crucial. This paper first indicates that for a given network, such cost is not only related to the attribution of environmental constraints but also related to the considered control measures. Next, we present an assessment criterion that quantifies the loss of optimality under the control measures considered by introducing the environmental constraints. The criterion can be acquired by solving a bi-level programming problem with/without environmental constraints. A simple case study shows its practicability as well as the differences between this framework and other frameworks integrating the environmental aspects. This proposed framework is widely applicable when assessing the interaction of traffic and its environmental aspects.     

基于灰关联混合蛙跳算法的雷达波形设计

提出了一种基于灰关联混合蛙跳算法的雷达波形设计方法,以混合蛙跳算法为主体,在局部更 新算子中引入遗传算法的遗传算子,并改进原始蛙跳算法的分组方法,丰富了种群的多样性,同时引入灰关联综合评价法则对适应度函数值加以关联度分析。文中以设计具有低自相关旁瓣和互相关特性的正交多相编码为例,将该算法用于雷达波形设计中。仿真结果表明使用本文算法产生 的波形具备较好的低自相关特性和互相关特性,表明了该算法是有效和可行的。     

Aggregate‐induced emission in light‐emitting liquid crystal display technology

Nowadays, liquid crystal displays (LCDs) with light‐emission are considered as energy‐efficient devices and are promising alternatives to conventional LCDs. To realize such possibility, strong fluorescent materials with a dichroic properties are required. Aggregate‐induced emission (AIE) is an unusual photophysical phenomenon shown by some luminogenic materials that will be highly emissive in their aggregate state. In this work, we studied the AIE effect of a luminescent liquid crystalline molecule TPE‐PPE in our LC system as a luminophore dopant. The result showed the excellent AIE effect that higher concentration of luminogen in the nematic LC host induced stronger luminescent intensity. Through exposure of a photoisomeriable alignment material sulfonic‐dye‐1, the photopatterning of a light‐emitting LC device was achieved with the use of the TPE‐PPE/nematic LC mixture.