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Mariana C. S. Vallejo Nuno M. M. Moura Maria Amparo Ferreira Faustino Adelaide Almeida Idalina Gonalves Vanda V. Serra Maria Graa P. M. S. Neves 《International journal of molecular sciences》2021,22(1)
The concept behind photodynamic therapy (PDT) is being successfully applied in different biomedical contexts such as cancer diseases, inactivation of microorganisms and, more recently, to improve wound healing and tissue regeneration. The effectiveness of PDT in skin treatments is associated with the role of reactive oxygen species (ROS) produced by a photosensitizer (PS), which acts as a “double agent”. The release of ROS must be high enough to prevent microbial growth and, simultaneously, to accelerate the immune system response by recruiting important regenerative agents to the wound site. The growing interest in this subject is reflected by the increasing number of studies concerning the optimization of relevant experimental parameters for wound healing via PDT, namely, light features, the structure and concentration of the PS, and the wound type and location. Considering the importance of developing PSs with suitable features for this emergent topic concerning skin wound healing, in this review, a special focus on the achievements attained for each PS class, namely, of the non-porphyrinoid type, is given. 相似文献
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Euclides J. Lourenco Adelaide T. Catutani 《Journal of the science of food and agriculture》1984,35(10):1120-1127
Pectinesterase (PE) was partially purified from papaya pulp, and its biochemical properties were studied. The enzyme was eluted in a single peak after DEAE-cellulose and Sephadex G-100 chromatography. The PE had a molecular weight of 53000 and showed an optimum pH of 8.0. Its activity was dependent on an NaCl concentration of 0.2M . The enzyme was heat stable: approximately 80% of the original activity remained after 60 min of heating at 50°C but completely inactivated by incubation at 80°C for 1 min. The activity was linear with time and protein concentration. The maximum reaction in 3 min was found at 60°C and the initial rate increased 9-fold from 20 to 60°C. The estimated Km was 0.12g litre?1 with citrus pectin as the substrate. The kinetic study revealed that polygalactur-onic acid is a competitive inhibitor, and a Ki value of 0.07 g litre?1 was determined. On the basis of this study, papaya PE properties resembled those of pectinesterase from other sources. 相似文献
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Anna Karamysheva;José M. Silva;William M. Facchinatto;Márcia D. Braz;Adelaide Almeida;Armando J. D. Silvestre;Carmen S. R. Freire;Carla Vilela; 《应用聚合物科学杂志》2024,141(32):e55767
The design of eco-friendly active food packaging systems based on biobased polymeric materials is a growing field of research because of the pressing concern for sustainable development. Herein, monolayer ternary thermoplastic films composed of poly(lactic acid) (PLA), gallic acid (GA, 2.5% w/w relative to PLA), and a plasticizer (poly(ethylene glycol) [PEG] or epoxidized linseed oil [ELO], 5.0 and 7.5% w/w relative to PLA) are prepared via melt-mixing followed by compression molding. The ensuing plasticized and self-standing PLA/GA films are translucent and have good mechanical properties (Young's modulus ~2 GPa) and welding performance. The incorporation of GA yields films with ultraviolet-light barrier properties (transmittance below 40%, 200–400 nm), antioxidant capacity (radical scavenging activity above 94%), and antibacterial activity against the methicillin-resistant strain of Staphylococcus aureus (minimum of 3-log reduction colony forming units mL−1). These thermoplastic films are easily degradable via enzymatic degradation with proteinase K from Tritirachium album, reaching weight loss values of 100% after 9 days. The films plasticized with PEG present slightly better antibacterial action and enzymatic degradation, whereas those plasticized with ELO exhibit marginally higher surface hydrophobicity and thermal stability. Thus, the combination between PLA and GA yielded biobased films with bioactive properties that have potential for application in active food packaging. 相似文献
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Adelaide Cerveira Amaro de Sousa E. J. Solteiro Pires José Baptista 《International Transactions in Operational Research》2024,31(1):88-114
Wind power is becoming an important source of electrical energy production. In an onshore wind farm (WF), the electrical energy is collected at a substation from different wind turbines through electrical cables deployed over ground ditches. This work considers the WF layout design assuming that the substation location and all wind turbine locations are given, and a set of electrical cable types is available. The WF layout problem, taking into account its lifetime and technical constraints, involves selecting the cables to interconnect all wind turbines to the substation and the supporting ditches to minimize the initial investment cost plus the cost of the electrical energy that is lost on the cables over the lifetime of the WF. It is assumed that each ditch can deploy multiple cables, turning this problem into a more complex variant of previously addressed WF layout problems. This variant turns the problem best fitting to the real case and leads to substantial gains in the total cost of the solutions. The problem is defined as an integer linear programming model, which is then strengthened with different sets of valid inequalities. The models are tested with four WFs with up to 115 wind turbines. The computational experiments show that the optimal solutions can be computed with the proposed models for almost all cases. The largest WF was not solved to optimality, but the final relative gaps are small. 相似文献
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Ramadoss Janarthanan Kengne Jacques Telem Adelaide Nicole Kengnou Tsafack Nestor Rajagopal Karthikeyan 《Analog Integrated Circuits and Signal Processing》2022,111(3):495-495
Analog Integrated Circuits and Signal Processing - 相似文献