Synthesis and characterization of PMMA composites activated with starch for immobilization of L‐asparaginase

Poly (methyl methacrylate) (PMMA)–starch composites were prepared by emulsion polymerization technique for L‐asparaginase (L‐ASNase) immobilization as highly activated support. The hydroxide groups on the prepared composites offer a very simple, mild and firm combination for enzyme immobilization. The pure PMMA and PMMA‐starch composites were characterized as structural, thermal and morphological. PMMA‐starch composites were found to have better thermal stability and more hydrophilic character than pure PMMA. L‐ASNase was immobilized onto PMMA‐starch composites contained the different ratio of starch (1, 3, 5, and 10 wt %). Immobilized L‐ASNase showed better performance as compared to the native enzyme in terms of thermal stability and pH. K_m value of immobilized enzyme decreased approximately eightfold compared with the native enzyme. In addition to, immobilized L‐ASNase was found to retain 60% of activity after 1‐month storage period at 4 °C. Therefore, PMMA‐starch composites can be provided more advantageous in terms of enzymatic affinity, thermal, pH and storage stability as L‐ASNase immobilization matrix. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016 , 133, 43421.     

Functionalized Hybrid Coatings on ABS Surfaces by PLD and Dip Coatings

It is important to give water-repellent and antibacterial properties to the acrylonitrile butadiene styrene (ABS) surfaces of the hearing aids. In this study, the sol–gel Si and sol–gel Ti solutions were prepared from the reactions of silicon ethoxide, titanium butoxide and methacrylic acid. The catalyst and Dynasylan F8815 were added to the sol–gel solutions to give hydrophobic properties onto the ABS surfaces. Additionally, silver nanoparticles were synthesized by nanosecond laser and added to the coating solutions to give extra antibacterial properties. The surfaces of the ABS targets were coated using the sol–gel dip coating and pulsed laser deposition techniques. The coatings with good adhesion between film and substrate and good water-repellent properties were achieved. The average contact angles for the coated ABS surfaces were measured in the range between 120 and 125 degrees. The obtained sol–gel materials and produced thin films onto the ABS surfaces were also analyzed in terms of the antibacterial properties. The highly antibacterial properties were observed in the sol–gel solutions and the thin films.     

Fatty acids of the seeds of <Emphasis Type="Italic">Origanum onites</Emphasis> L. and <Emphasis Type="Italic">O. vulgare</Emphasis> L.

Seed oils of Origanum onites L. from the Antalya and Mugla regions and O. vulgare L. from the Kirklareli region of Turkey were extracted with hexane in a Soxhlet apparatus. The oil yields were 14.1–20.0 and 18.5%, respectively. FA compositions of the seed oils were determined by GC and GC/MS. Twenty FA were identified in both O. onites and O. vulgare seeds. The major FA of both species were linolenic (56.3–57.0%; 61.8%), linoleic (21.5–21.7%; 18.8%), oleic (8.7–8.9%; 5.9%), palmitic (5.9–6.5%; 5.5%), stearic (2.1–2.4%; 2.1%), and (Z)-11-octadecenoic (0.6–0.8%; 0.5%), respectively.     

Selective separation of cobalt and nickel by flat sheet supported liquid membrane using Alamine 300 as carrier

Cobalt and nickel are among the most important nonferrous metals. The using of flat sheet supported liquid membranes (FSSLMs) to remove metals from wastewaters has been used actively by the scientific and industrial communities. In this study, the selective separation of cobalt from thiocyanate solutions containing cobalt and nickel by FSSLM was examined using tri-n-octylamine (Alamine 300) as carrier. The FSSLM was consisted of extractant, flat sheet support and organic solvent. The various parameters were studied to determine the optimum extraction and striping conditions of cobalt and nickel. These parameters were stirring speeds of phases, NH₄SCN concentration, pH, diluent type, extractant concentration, stripping reagent concentration and modifier concentration. Concentration of cobalt and nickel were determined by Shimadzu AA-6701GF spectrophotometer. In the optimum conditions, selective separation of cobalt was achieved with an efficiency of 98.4% within 8 h, for equimolar feed mixtures, 400 mg/L Co + 400 mg/L Ni, and the separation factor of Co(II) over Ni(II) was 234.4. In addition, for nonequimolar feed mixtures, 500 mg/L Co + 1000 mg/L Ni, Ni in excess, selective separation of cobalt was 99.9%, and the separation factor of Co was 506 in the same time.     

Chemical Profile and Biological Activities of Two Edible Plants: Chemical Investigation and Quantitative Analysis Using Liquid Chromatography Tandem Mass Spectrometry and Gas Chromatography Mass Spectrometry

The objectives of this study were to define the phenolic and fatty acid profiles, anticholinesterase, antioxidant, antimicrobial activities, and total phenolic-flavonoid contents of Lycopsis orientalis and Tragopogon latifolius var. angustifolius which have been used as food source and food supplement in Anatolia and have never been examined before. Rosmarinic and quinic acids (21.11 and 11.46 mg g^–1 extract, respectively) were found to be the most abundant constituents in L. orientalis and T. latifolius var. angustifolius among the studied 27 compounds by liquid chromatography tandem mass spectrometry. In the fatty acid compositions of L. orientalis and T. latifolius var. angustifolius that were determined by gas chromatography mass spectrometry, oleic (29.1%) and palmitic (28.7%) acids were identified as the major components, respectively. The high antioxidant activity of the methanol extract of L. orientalis shows parallelism to its rosmarinic acid content. Besides, this extract showed medium anticholinesterase activity. The results of the present study proves that the L. orientalis might also be used as a food source due to its high phenolic acid content and strong antioxidant property.     

Entropy generation in the flow system generated in between two parallel plates due to bivertical motion of the top plate

Thermodynamic irreversibility in the flow system provides information on the energy and power losses in the system. Minimization of entropy generation in the flow system enables for the parametric optimization of the system operation. In the present study, parallel plates, in between, filled with the fluid are considered. The fluid motion resulted from the bi-vertical compression of the top plate of the parallel plates is examined. The entropy generation rate in the flow system is formulated after considering the constant movement of the top plate, constant applied load, and the combination of the constant velocity and applied load to the top plate. The optimum operating conditions related to the fluid motion in between the parallel plates is determined through the entropy analysis. It is found that the combination of the constant velocity and the constant applied load resulted in the low entropy generation rate.     

Diffusion bonding of electroless Ni plated WC composite to Cu and AISI 316 stainless steel

In this study, a composite containing WC (Tungsten Carbide) and Ni was produced by two different processing routes. Electroless Ni coated WC powders were consolidated and sintered at 1200 °C. Diffusion bonding couples of WC(Ni)-electrolytic Cu, WC(Ni)-AISI 316 stainless steel and WC(Ni)-WC(Ni) were manufactured by using a preloaded compression system under Ar atmosphere. Diffusion bonding was carried out at varying bonding temperatures; 750 °C for (WC)Ni-Cu diffusion couple and 1200 °C for (WC)Ni-(WC)Ni and (WC)Ni-AISI 316 stainless steel diffusion couples. Standard metallographic techniques, Scanning Electron Microscopy and a shear test were employed to characterize the microstructure of bondline and mechanical properties of each diffusion couple, respectively.     

RFID behavior study in enclosed marine container for real time temperature tracking

The performance of radio waves in open environments has been studied for years. In contrast, the behavior of Radio Frequency Identification (RFID) inside metal enclosed areas is not yet understood. This research project focuses on the 3-D mapping of RFID signal strength inside a 12 m refrigerated marine container instrumented with three different types of radio frequency (RF) emitters: 915 MHz reader; 2.45 GHz reader and 433 MHz RF transmitter. The main goal is to find a frequency/configuration that would allow real time reading of the temperature in a shipment of perishable products using RFID. Only one frequency and one antenna were used at a time. The RF transmitter antenna was mounted at two different places inside the container; at the top of the front wall (facing back) and on the ceiling in the middle of the container (facing down). The signal strength was acquired by a spectrum analyzer and its antenna was mounted on a small electric cart inside the container. The cart was programmed to move along the length of the container and stop repeatedly, allowing three automated measures per position. All data were analyzed in terms of power level and attenuation. The maps showed that the RFID antenna positioned at the front of the container delivered slightly better results than the one in the middle of the ceiling. The results showed a significantly higher performance at the 433 MHz level. This article was presented at Food Processing Automation Conference, American Society of Agricultural and Biological Engineering (ASABE), Providence, RI, June 28–29 2008.