Suppression of inducible nitric oxide synthase expression and amelioration of lipopolysaccharide-induced liver injury by polyphenolic compounds in Eucalyptus globulus leaf extract

Eucalyptus leaf extract (ELE) is rich in hydrolyzable tannins. We examined the effects of ELE and its constituents on lipopolysaccharide (LPS)-induced liver injury in mice. Mice fed a diet supplemented with 1% ELE were intraperitoneally administered LPS. Six hours later, the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were significantly lower in the ELE-supplemented mice than in the controls; LPS-induced hepatic inducible nitric oxide synthase (iNOS) expression was also suppressed. ELE lowered LPS-stimulated iNOS expression in cultured RAW 264.7 macrophages. Furthermore, the aglycones of hydrolyzable tannins, gallic acid (GA) and ellagic acid (EA), inhibited iNOS induction to a greater extent than did ELE (15-fold higher). When mice were fed a 1% GA or EA diet, the increase in the serum ALT and AST activities and hepatic iNOS expression in response to the LPS challenge were significantly attenuated. Thus, hydrolyzable tannins in ELE ameliorate LPS-induced liver injury.     

Development of Ultra Low Field Nuclear Magnetic Resonance Imaging System Using HTS rf SQUID

We constructed and studied an ultra low-field nuclear magnetic resonance imaging system using a HTS rf SQUID and room-temperature coils. We recorded the free induction decay signals of ¹H by employing a measuring field, B _m, of 44.8???T and a polarizing field, B _p, of 36.8?mT; in the presence of ? B _Z /? z and ? B _Z /? y gradients of the order of 0.8???T/m, we were able to obtain a quasi two-dimensional 4-pixels image of a simple water phantom.     

Multiscale modeling in rodent ventricular myocytes

This paper indicates that in ventricular myocytes when the SR (sarcoplasmic reticulum) is pharmacologically inhibited, the intracellular Ca²⁺concentration rapidly increases during Ca²⁺ entrance (0-70 ms), whereas the decay of Ca²⁺ is slow; in the absence of fluorescent dye, large Ca²⁺ concentration gradients might develop near the cell membrane; intracellular Ca²⁺ distribution is tightly regulated by the localization of Ca²⁺transporter proteins along the sarcolemma and strongly relays on the presence of mobile and stationary Ca²⁺ buffers. These studies also imply that in ventricular cells with intact and functional SR, the Ca²⁺ signal most likely would spread faster along the t-tubular system, surface membrane than to the cell interior and that in the absence of Ca²⁺ dye high Ca²⁺ gradients under the surface membrane and more uniform Ca²⁺ distribution in the cell interior might be expected.     

Magnetic and EPR studies of edge-localized spin paramagnetism in multi-shell nanographites derived from nanodiamonds

Prolonged (up to 2 h) heat treatment at 1600 °C of nanodiamond particles (5 nm) leads to their conversion to the mixture of quasi-spherical carbon onions and multi-shell polyhedral nanographites. Structural and magnetic properties of two (A and B) series of nanographite samples obtained at various annealing intervals were studied. XRD data show that both multi-shell nanographite samples have practically the same crystalline structures. HRTEM evidences that the most of particles obtained by short time (7 min) annealing have a spherical like shape whereas the long time (~ 2 h) annealing leads to the majority of particles having a polyhedral shape with a hollow inside. Electronic and magnetic properties of these nanocarbons were investigated by magnetic susceptibility and EPR. Annealing results in entire transformation of the EPR signal of nanodiamond to new EPR signals of various line shapes and widths. These signals are extremely sensitive to ambient oxygen. Concentrations for all EPR active spins vary from ~ 1 × 10¹⁹ spins/g (7 min) to ~ 2 × 10¹⁹ spins/g (2 h). Temperature dependences of EPR spectra' parameters were obtained for vacuum-processed samples within the range 4?600 K. Intensity vs. T plots may be well-fitted by the combination of Curie–Weiss and temperature-independent Pauli susceptibility contributions. The latter one increases on heat treatment. Significant extension of electron spin-lattice relaxation time on decreasing temperature was found.     

Surface modification of an organosilane self-assembled monolayer on silicon substrates using atomic force microscopy: scanning probe electrochemistry toward nanolithography

Organosilane self-assembled monolayers (SAMs) have been applied to resist materials for nanolithography based on scanning probe microscopy. An organosilane SAM was prepared on Si substrates from a precursor, that is octadecyltrimethoxysilane. Using an atomic force microscope with a conductive probe, current was injected from the probe into the SAM-covered Si substrate so that the SAM was locally degraded at the probe-contacting point. Nanoscale patterns drawn on the SAM was clearly imaged by lateral force microscopy. The patterning could be conducted in air while, in vacuum at the order of 10(-6) Torr, no detectable patterns were fabricated. The presence of adsorbed water at the probe/sample junction was confirmed to be crucial for the patterning of the SAM/Si. Its mechanism was, thus, ascribed to electrochemical reactions of both the SAM and Si with adsorbed water.     

An investigation of the pH-activity relationships of Cex, a family 10 xylanase from Cellulomonas fimi: xylan inhibition and the influence of nitro-substituted aryl-beta-D-xylobiosides on xylanase activity

The kinetic parameters of Cex, a family 10 xylanase from Cellulomonas fimi, were determined at various pH levels using soluble birchwood xylan (BWX) as a natural polymeric substrate along with three other synthetic aryl-beta-D-xylobioside substrates. Using BWX, a high level of substrate inhibition was observed which increased with decreasing pH. In contrast, typical Michaelis-Menten-type profiles were obtained using the three aryl-beta-D-xylobiosides as substrates. The k(cat) values determined using o-nitrophenyl-beta-D-xylobioside did not change as the pH increased, whereas the k(cat) values obtained with BWX, phenyl-beta-D-xylobioside and p-nitrophenyl-beta-D-xylobioside decreased, suggesting that the presence of an ortho nitro group affects the activity displayed by Cex. These differences were not observed with XynB from Clostridium stercorarium F9, a member of the same family of xylanases as Cex. These results indicate that a careful evaluation is required when employing substituted aryl-beta-D-xylobiosides in the characterization of xylanases.     

Mathematics based on incremental learning—Excluded middle and inductive inference

Learning theoretic aspects of mathematics and logic have been studied by many authors. They study how mathematical and logical objects are algorithmically “learned” (inferred) from finite data. Although they study mathematical objects, the objective of the studies is learning. In this paper, a mathematics whose foundation itself is learning theoretic will be introduced. It is called Limit-Computable Mathematics. It was originally introduced as a means for “Proof Animation”, which is expected to make interactive formal proof development easier. Although the original objective was not learning theoretic at all, learning theory is indispensable for our research. It suggests that logic and learning theory are related in a still unknown but deep new way.