Object
Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics.
T 2 * mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short
T 2 * . We aimed to quantify
T 2 * in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice.
Materials and methods
I/R-injury was induced in C57BL/6 mice (
n?=?9). Sham-operated mice (
n?=?8) served as controls. MRI was performed at baseline, and 1, 7 and 28?days after surgery. MRI at 9.4?T consisted of Cine,
T 2 * mapping and late-gadolinium-enhancement (LGE). Mice (
n?=?6) were histologically assessed for hemorrhage and collagen in the fibrotic scar.
Results
Baseline
T 2 * values were 17.1?±?2.0?ms. At day 1, LGE displayed a homogeneous infarct enhancement.
T 2 * in infarct (12.0?±?1.1?ms) and remote myocardium (13.9?±?0.8?ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous.
T 2 * in the infarct decreased to 7.9?±?0.7 and 6.4?±?0.7?ms, whereas
T 2 * values in the remote myocardium were 14.2?±?1.1 and 15.6?±?1.0?ms. Histology revealed deposition of iron and collagen in parallel with decreased
T 2 * .
Conclusion
T 2 * values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase,
T 2 * values in infarcted myocardium differ significantly from those in the chronic phase.
T 2 * mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence,
T 2 * may be used to discriminate between acute and chronic infarctions.
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