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排序方式: 共有122条查询结果,搜索用时 15 毫秒
71.
Tarl W. Prow Xianfeng Chen Natalie A. Prow Germain J. P. Fernando Cindy S. E. Tan Anthony P. Raphael David Chang Merja P. Ruutu Derek W. K. Jenkins Alyssa Pyke Michael L. Crichton Kristin Raphaelli Lucas Y. H. Goh Ian H. Frazer Michael S. Roberts Joy Gardner Alexander A. Khromykh Andreas Suhrbier Roy A. Hall Mark A. F. Kendall 《Small (Weinheim an der Bergstrasse, Germany)》2010,6(16):1776-1784
The ‘Nanopatch’ (NP) comprises arrays of densely packed projections with a defined geometry and distribution designed to physically target vaccines directly to thousands of epidermal and dermal antigen presenting cells (APCs). These miniaturized arrays are two orders of magnitude smaller than standard needles—which deliver most vaccines—and are also much smaller than current microneedle arrays. The NP is dry‐coated with antigen, adjuvant, and/or DNA payloads. After the NP was pressed onto mouse skin, a protein payload co‐localized with 91.4 ± 4.1 APC mm−2 (or 2925 in total) representing 52% of the delivery sites within the NP contact area, agreeing well with a probability‐based model used to guide the device design; it then substantially increases as the antigen diffuses in the skin to many more cells. APC co‐localizing with protein payloads rapidly disappears from the application area, suggesting APC migration. The NP also delivers DNA payloads leading to cutaneous expression of encoded proteins within 24 h. The efficiency of NP immunization is demonstrated using an inactivated whole chikungunya virus vaccine and a DNA‐delivered attenuated West Nile virus vaccine. The NP thus offers a needle‐free, versatile, highly effective vaccine delivery system that is potentially inexpensive and simple to use. 相似文献
72.
Ayou Hao Iris Wong Hao Wu Brian Lisco Brian Ong Alyssa Sallean Steven Butler Michelle Londa Joseph H. Koo 《Journal of Materials Science》2015,50(1):157-167
This study focused on the influence of different filler loadings on the elongation at break and flammability properties of the PA11/FR/HNTs nanocomposites. Polyamide 11 (PA11)/flame-retardant (FR) additives/halloysite nanotubes (HNTs) nanocomposites were melt compounded via twin-screw extrusion for all the compositions. Three FR additive loadings (15, 20, and 25 wt%) and three HNTs loadings (2.5, 5, and 10 wt%) were selected. The formula with 25 % FR and 2.5 % HNT had the lowest additives content and the highest elongation at break of 10.22 % among all UL-94 V-0 rated formulas. A homogeneous dispersion of HNTs in PA11 matrix was observed by transmission electron microscopy. Differential scanning calorimeter measurements indicated that HNTs behaved as nucleating agents by accelerating the rate of crystallization, thus increasing crystallization temperature. The young’s modulus of the PA11 nanocomposites was enhanced with the addition of HNTs. Micro-scale combustion calorimeter results demonstrated that the addition of HNTs also decreased the peak heat release rate of the nanocomposites. These results indicate the effectiveness of HNTs on the mechanical, thermal, and flame-retardant performance of PA11/FR/HNTs nanocomposites. 相似文献
73.
Dimkpa CO Calder A Gajjar P Merugu S Huang W Britt DW McLean JE Johnson WP Anderson AJ 《Journal of hazardous materials》2011,188(1-3):428-435
This study explores the potential antimicrobial mechanisms of commercial silver nanoparticles (Ag NPs) in the environmental bacterium, Pseudomonas chlororaphis O6. The 10nm size NPs aggregated in water, as demonstrated by atomic force microscopy. Solubility of the NPs at 10mg/L was 0.28 mg/L (pH 6) and 2.3mg/L (pH 7); release from 10mg/L bulk Ag was below detection. The NPs eliminated cell culturability at 3mg/L, whereas no effect was observed at 10mg/L bulk Ag. Zeta potential measurements revealed that the NPs were negatively charged; unlike Ag ions, their addition to the negatively charged cells did not change cell charge at pH 6, but showed a trend to reduce cell charge at pH 7. Isolated extracellular polymeric substances (EPS) from PcO6 was polydisperse, with negative charge that was neutralized by Ag ions, but not by the NPs. Addition of EPS eliminated Ag NP's toxicity in cells lacking EPS. Intracellular accumulation of OH was not detected in NP-treated cells; however, the use of scavengers suggested the NPs caused extracellular H(2)O(2) production. No evidence was found for loss of membrane integrity upon treatment with the NPs. Our findings indicate that growth of environmental bacteria could be impaired by Ag NPs, depending on the extent of EPS production. 相似文献
74.
Jennifer S. Coelho Alyssa Idler Carolina O.C. Werle Anita Jansen 《Food quality and preference》2011,22(8):780-784
Food-scented products are widely available, yet it is unclear whether they influence eating behavior. Food-related cues can increase intake; therefore, it was anticipated that conscious exposure to food-scented products (e.g., body lotion) could also influence consumption. Female participants (n = 58) were randomly assigned to an exposure condition (labeled chocolate lotion, unlabeled chocolate lotion, or unscented lotion), and their subsequent intake of chocolate-chip cookies was measured. A significant effect of condition on intake emerged. Those who knew that they were evaluating a chocolate-scented lotion ate more than did those exposed to the same (unlabeled) lotion, suggesting that conscious exposure to chocolate-related products may increase food intake. 相似文献
75.
Einkorn (Triticum monococcum ssp. monococcum) is an underutilised wheat with high protein, lutein and tocols content, particularly suited for baby and specialty foods. To study the influence of kernel steaming treatments on chemical and technological properties, seeds of five einkorn accessions and one bread wheat control (cv. Blasco) underwent different steaming conditions, and their wholemeal flours were compared for ash, protein, lutein, tocols and α-amylase content, SDS sedimentation volume and gelatinisation parameters. Furosine, a heat damage marker, was measured as well. The treatments significantly influenced most of the parameters. In particular, lutein, tocols and α-amylase diminished after steaming; SDS sedimentation volumes and most gelatinisation parameters also decreased, whereas gelatinisation temperatures and furosine contents increased. The changes were stronger under more drastic steaming conditions, although treatment × genotype influence was sometimes detected. Steaming induced migration of lutein and tocopherols from the bran and germ fractions to the kernel endosperm. 相似文献
76.
Alyssa Farrell 《中国计算机用户》2008,(29):64-65
通过将人、环保和利润统一起来,企业可以将可持续发展战略转变为竞争优势。车库中的混合动力汽车,食品储藏室内的咖啡豆,精确管理的恒温器,打印机中重复利用的墨盒。 相似文献
77.
Bridges Ana J.; de Arellano Michael A.; Rheingold Alyssa A.; Danielson Carla Kmett; Silcott Lauren 《Canadian Metallurgical Quarterly》2010,2(1):40
Although the largest immigrant group in the United States is Hispanic, little is known about their rates of traumatic experiences and psychiatric disorders, particularly for youth. Findings with adults suggest that recent immigrants have lower rates of mental illness than long-time residents or U.S.-born Hispanics, but use health-related services less often. The present study examined this relationship in a convenience sample of 131 foreign-born (64.5%) and 72 U.S.-born (35.5%) Hispanic youth, ages 8–17 years and a subset of their caregivers (n = 110). Findings from youth interview data suggest that immigrant and U.S.-born youth did not differ significantly in experiences of potentially traumatizing events or psychiatric disorders. However, findings from caregiver interview data suggest that there were significant disparities between the two groups in health service utilization for doctors and other medical professionals, with caregivers reporting that foreign-born youth utilize these health services at lower rates than U.S.-born youth. Results are discussed in the context of prior findings and recommendations offered for increasing service utilization. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
78.
Alyssa Friend Wise Simone Nicole Hausknecht Yuting Zhao 《International Journal of Computer-Supported Collaborative Learning》2014,9(2):185-209
Theoretical models of collaborative learning through online discussions presuppose that students generally attend to others’ posts. However, a succession of studies over the last decade has shown this assumption to be unwarranted. Instead, research indicates that learners attend to others’ posts in diverse and particular ways—an activity we have conceptualized as online “listening.” In this study, we take an important step forward in developing a robust theory of online listening by examining the relationship between how learners “listen” (access existing posts) and “speak” (contribute posts) in online discussions. Ten variables indexing four dimensions of students’ listening (breadth, depth, temporal contiguity and revisitation) and five variables indexing three dimensions of students’ speaking (discursiveness, depth of content and reflectivity) were calculated for 31 students participating in 6 week-long online discussions as part of an undergraduate educational psychology course. Multi-level mixed-model linear regressions indicated that responsiveness of students’ posts was positively predicted by how often they revisited previously read peer posts, and negatively related to a greater number of posts in the discussion overall. The depth of posts’ contents was predicted by the percentage of posts viewed that students actually read (as opposed to scanned). An exploratory follow-up analysis indicated that these listening-speaking relationships manifest differently over time for distinct subsets of learners (e.g., a decrease in variable pairs versus corresponding fluctuations around stable levels). Put together, results suggest that when students take the time to read and re-read their peers’ posts there are related benefits in the quality of the posts they contribute. 相似文献
79.
Alyssa B. Chinen Chenxia M. Guan Caroline H. Ko Chad A. Mirkin 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(16)
The effect of serum protein adsorption on the biological fate of Spherical Nucleic Acids (SNAs) is investigated. Through a proteomic analysis, it is shown that G‐quadruplexes templated on the surface of a gold nanoparticle in the form of SNAs mediate the formation of a protein corona that is rich in complement proteins relative to SNAs composed of poly‐thymine (poly‐T) DNA. Cellular uptake studies show that complement receptors on macrophage cells recognize the SNA protein corona, facilitating their internalization, and causing G‐rich SNAs to accumulate in the liver and spleen more than poly‐T SNAs in vivo. These results support the conclusion that nucleic acid sequence and architecture can mediate nanoparticle–biomolecule interactions and alter their cellular uptake and biodistribution properties and illustrate that nucleic acid sequence is an important parameter in the design of SNA therapeutics. 相似文献
80.
Alyssa Maria Pearce 《International journal of human-computer interaction》2017,33(3):180-189
This study examines how players of World of Warcraft, an online video game, use language to perform gendered identities. Online environments have become increasingly popular sites for social interaction, and online games are played by millions of people worldwide who must rely on textual language to construct their identity. Data were collected from semi-private chat channels by recording visual game-play while conducting participant observation. The chat log data were analyzed in terms of gender stereotypes known to exist in video games using conversation analytic techniques. Players were observed using gendered stereotypes, as well as language practices that have been prescribed as being male- or female-orientated. How identity is constructed through language in online video games has become increasingly relevant as the importance of individual identity has risen in many Western cultures as well as in academia. 相似文献