Real-time air monitoring of occupational exposures to particulate matter among hairdressers in Maryland: A pilot study

Hairdressers are exposed to particulate matter (PM), a known air pollutant linked to adverse health effects. Still, studies on occupational PM exposures in hair salons are sparse. We characterized indoor air PM concentrations in three salons primarily serving an African/African American (AA) clientele, and three Dominican salons primarily serving a Latino clientele. We also assessed the performance of low-cost sensors (uRAD, Flow, AirVisual) by comparing them to high-end sensors (DustTrak) to conduct air monitoring in each salon over 3 days to quantify work shift concentrations of PM_2.5, respirable PM (RPM), and PM₁₀. We observed high spatial and temporal variability in 30-min time-weighted average (TWA) RPM concentrations (0.18–5518 μg/m³). Readings for the uRAD and AirVisual sensors were highly correlated with the DustTrak (R² = 0.90–0.99). RPM 8-hour TWAs ranged from 18 to 383 µg/m³ for AA salons, and 9–2115 µg/m³ for Dominican salons. Upper 95th percentiles of daily RPM exposures ranged from 439 to 2669 µg/m³. The overall range of 30-min TWA PM_2.5 and PM₁₀ concentrations was 0.13–5497 and 0.36-,541 μg/m³, respectively. Findings suggest that hairdressers could be overexposed to RPM during an 8-hour shift. Additional comprehensive monitoring studies are warranted to further characterize temporal and spatial variability of PM exposures in this understudied occupational population.     

Prisoners of Ritoque: The Open City and the Ritoque Concentration Camp

In the early 1970s, a school of architecture and a concentration camp appeared at the Ritoque beach, just north of Valparaíso, Chile. Situated three miles apart, they never acknowledged each other's presence. Nonetheless, their occupants formed communities that used a similar repertoire of games, events, and performances to create real and imaginary spaces. Faculty at the school deployed these activities to form a utopian enclave, freeing students and themselves from the strictures of normative education and practice, while limiting their political agency. In contrast, the prisoners of the camp transformed their enforced isolation into active political resistance.     

Antibiotic resistance in coagulase negative staphylococci isolated from wastewater and drinking water

This study reports the antibiotic resistance patterns of coagulase negative staphylococci (CNS) isolated from a drinking water treatment plant (WTP), a drinking water distribution network, responsible for supplying water to the consumers (WDN), and a wastewater treatment plant (WWTP), responsible for receiving and treating domestic residual effluents. Genotyping and the 16S rRNA gene sequence analysis demonstrated a higher diversity of species both in the WTP (6 species/19 isolates) and WWTP (12 species/47 isolates) than in the WDN (6 species/172 isolates). Staphylococcus pasteuri and Staphylococcus epidermidis prevailed in the WTP and WDN and Staphylococcus saprophyticus in the WWTP. Staphylococci with reduced susceptibility (resistance or intermediary phenotype) to beta-lactams, tetracycline, clindamycin and erythromycin were observed in all types of water and belonged to the three major species groups. The highest resistance rate was found against erythromycin, presumably due to the presence of the efflux pump encoded by the determinant msrA, detected in the majority of the resistant isolates. This study demonstrates that antibiotic resistant CNS may colonize different types of water, namely drinking water fulfilling all the quality standards.     

Displacement and load transfer mechanisms of geogrids under pullout condition

Reinforcing elements embedded within soil mass improve stabilization through a load transfer mechanism between the soil and the reinforcement. Geogrids are a type of geosynthetic frequently used for soil reinforcement, consisting of equally spaced longitudinal and transverse ribs. Under pullout conditions, the longitudinal ribs are responsible for tensile resistance, while transverse ribs contribute to a passive resistance. This paper describes a new analytical model capable of reproducing both load transfer and displacement mechanisms on the geogrid length, under pullout conditions. The model subdivides the geogrid into rheological units, composed by friction/adhesion and spring elements, mounted in line. Friction/adhesion elements respond to the shear component mobilized at the soil–geogrid interface. Spring elements respond to the geogrid's tensile elongation. Model parameters are obtained through tensile strength tests on geogrids and conventional direct shear tests on soil specimens. The need for instrumented pullout tests becomes therefore eliminated. Results predicted from this new model were compared to instrumented pullout test data from two types of geogrids, under various confining stress levels. The results revealed that the new model is capable of reasonably predicting load and displacement distributions along the geogrid.     

Biodeterioration of the Lions Fountain at the Alhambra Palace, Granada (Spain)

Stone works of art exposed to the environment are liable to be deteriorated by the action of biological agents such as bacteria, fungi, mosses, etc. In ornamental fountains, the microorganisms present in water can contribute to these biodeterioration processes. This paper assesses the biodeterioration experienced by the Lions Fountain at the Alhambra Palace in Granada (Spain). Analyses have been made of the biodeterioration of Lions 4, 5 and 9, the biofouling of the fountain basin, and the water supply system. Conventional and molecular biology techniques have identified microorganisms belonging to various microbial groups (-, β- and γ-Protebacteria, Chlamydiae/Verrucomicrobia and Eukaryota). Additionally, on the mortar in the sculptures the presence of algae and bryophytes has been observed. X-ray diffraction allowed both the detection of neoformation mineral products that can be related with the presence of microorganisms and the corrosion products in the Lions Fountain. A number of recommendations are made regarding the prevention and control of biodeterioration in this important work of art.     

Artificial neural networks for machining processes surface roughness modeling

In recent years, several papers on machining processes have focused on the use of artificial neural networks for modeling surface roughness. Even in such a specific niche of engineering literature, the papers differ considerably in terms of how they define network architectures and validate results, as well as in their training algorithms, error measures, and the like. Furthermore, a perusal of the individual papers leaves a researcher without a clear, sweeping view of what the field’s cutting edge is. Hence, this work reviews a number of these papers, providing a summary and analysis of the findings. Based on recommendations made by scholars of neurocomputing and statistics, the review includes a set of comparison criteria as well as assesses how the research findings were validated. This work also identifies trends in the literature and highlights their main differences. Ultimately, this work points to underexplored issues for future research and shows ways to improve how the results are validated.     

A multivariate surface roughness modeling and optimization under conditions of uncertainty

Correlated responses can be written in terms of principal component scores, but the uncertainty in the original responses will be transferred and will influence the behavior of the regression function. This paper presents a model building strategy that consider the multivariate uncertainty as weighting matrix for the principal components. The main objective is to increase the value of R² predicted to improve model’s explanation and optimization results. A case study of AISI 52100 hardened steel turning with Wiper tools was performed in a Central Composite Design with three-factors (cutting speed, feed rate and depth of cut) for a set of five correlated metrics (R_a, R_y, R_z, R_q and R_t). Results indicate that different modeling methods conduct approximately to the same predicted responses, nevertheless the response surface to Weighted Principal Component – case b – (WPC1^b) presented the highest predictability.     

Fibroblast growth factor,estrogen, and prolactin receptor features in different grades of prostatic adenocarcinoma in elderly men

The objective was to characterize and associate the receptor reactivities of fibroblastic growth factor (FGF)‐2, FGF‐7, FGF‐8, epidermal growth factor (EGF), α‐actin and vimentin in relation to the androgen receptor (AR), α and β estrogen receptors (ERα and ERβ), and prolactin receptor in the prostate of elderly men showing low‐ and high‐grade adenocarcinoma. Thirty prostatic samples were taken from 60‐ to 90‐year‐old patients without prostatic lesions and with low‐grade cancer and high‐grade cancer, from the University Hospital, School of Medicine, the State University of Campinas. The results showed that increased FGF‐2, FGF‐7, and FGF‐8 receptor reactivities and decreased AR reactivity were verified in both high‐ and low‐grade cancer. However, the FGF‐8 receptor showed greater involvement at the beginning of the malignancy alterations. Increased EGF receptor (EGFR) reactivity and diminished α‐actin immunohistochemistry were identified in both cancer groups. Also, increased ERα, PR, and vimentin receptors were verified in both cancer groups. To conclude, the ERα involvement in the reactive stroma activation led to a microenvironment, which was favorable to cancer progression, due to maximizing stromal imbalance. The prolactin could be related to cancer progression due to its interaction with ERα action, indicating that this hormone could be a relevant target to prevent the estrogenic effects in the prostatic lesions. Both FGF receptor (FGFR)‐2 and FGFR‐8 play a fundamental role in the early stages of prostate cancer, suggesting that these molecules could be a promising therapeutic target. The differential localization of the fibroblastic factors between the prostatic epithelium and stroma of elderly men, who presented prostate cancer, could indicate a favorable distinction for tumoral progression. Microsc. Res. Tech. 76:321–330, 2013. © 2013 Wiley Periodicals, Inc.     

A morphological analysis of the transition between the embryonic primitive intestine and yolk sac in bovine embryos and fetuses

The yolk sac (YS) is the main source of embryonic nutrition during the period when the placenta has not yet formed. It is also responsible for hematopoiesis because the blood cells develop from it as part of the primitive embryonic circulation. The objective of this study was to characterize the transitional area between the YS and primitive gut using the techniques of light microscopy, transmission electron microscopy, and immunohistochemistry to detect populations of pluripotent cells by labeling with Oct4 antibody. In all investigated embryos, serial sections were made to permit the identification of this small, restricted area. We identified the YS connection with the primitive intestine and found that it is composed of many blood islands, which correspond to the vessels covered by vitelline and mesenchymal cells. We identified large numbers of hemangioblasts inside the vessels. The mesenchymal layer was thin and composed of elongated cells, and the vitelline endodermal membrane was composed of large, mono‐ or binucleated cells. The epithelium of the primitive intestine comprised stratified columnar cells and undifferentiated mesenchymal cells. The transitional area between the YS and the primitive intestine was very thin and composed of cells with irregular shapes, which formed a delicate lumen containing hemangioblasts. In the mesenchyme of the transitional area, there were a considerable number of small vessels containing hemangioblasts. Using Oct4 as a primary antibody, we identified positive cells in the metanephros, primordial gonad, and hepatic parenchyma as well as in YS cells, suggesting that these regions contain populations of pluripotent cells. Microsc. Res. Tech. 76:756–766, 2013. © 2013 Wiley Periodicals, Inc.