全文获取类型
收费全文 | 5869篇 |
免费 | 539篇 |
国内免费 | 4篇 |
专业分类
电工技术 | 29篇 |
综合类 | 1篇 |
化学工业 | 2033篇 |
金属工艺 | 46篇 |
机械仪表 | 115篇 |
建筑科学 | 217篇 |
矿业工程 | 10篇 |
能源动力 | 171篇 |
轻工业 | 1820篇 |
水利工程 | 56篇 |
石油天然气 | 39篇 |
无线电 | 214篇 |
一般工业技术 | 724篇 |
冶金工业 | 301篇 |
原子能技术 | 11篇 |
自动化技术 | 625篇 |
出版年
2024年 | 18篇 |
2023年 | 93篇 |
2022年 | 357篇 |
2021年 | 406篇 |
2020年 | 210篇 |
2019年 | 250篇 |
2018年 | 252篇 |
2017年 | 243篇 |
2016年 | 280篇 |
2015年 | 209篇 |
2014年 | 310篇 |
2013年 | 461篇 |
2012年 | 427篇 |
2011年 | 460篇 |
2010年 | 344篇 |
2009年 | 334篇 |
2008年 | 287篇 |
2007年 | 255篇 |
2006年 | 191篇 |
2005年 | 146篇 |
2004年 | 128篇 |
2003年 | 99篇 |
2002年 | 89篇 |
2001年 | 52篇 |
2000年 | 63篇 |
1999年 | 61篇 |
1998年 | 81篇 |
1997年 | 72篇 |
1996年 | 36篇 |
1995年 | 27篇 |
1994年 | 23篇 |
1993年 | 21篇 |
1992年 | 15篇 |
1991年 | 6篇 |
1990年 | 9篇 |
1989年 | 22篇 |
1988年 | 7篇 |
1987年 | 12篇 |
1986年 | 5篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 3篇 |
1982年 | 5篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1978年 | 4篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1966年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有6412条查询结果,搜索用时 15 毫秒
61.
Maria Buuales Maria Cristina Ballesteros-Briones Manuela Gonzalez-Aparicio Sandra Hervas-Stubbs Eva Martisova Uxua Mancheo Ana Ricobaraza Sara Lumbreras Cristian Smerdou Ruben Hernandez-Alcoceba 《International journal of molecular sciences》2021,22(8)
Immune checkpoint inhibitors (ICIs) have demonstrated remarkable efficacy in a growing number of malignancies. However, overcoming primary or secondary resistances is difficult due to pharmacokinetics issues and side effects associated with high systemic exposure. Local or regional expression of monoclonal antibodies (mAbs) using gene therapy vectors can alleviate this problem. In this work, we describe a high-capacity adenoviral vector (HCA-EFZP-aPDL1) equipped with a mifepristone-inducible system for the controlled expression of an anti-programmed death ligand 1 (PD-L1) blocking antibody. The vector was tested in an immune-competent mouse model of colorectal cancer based on implantation of MC38 cells. A single local administration of HCA-EFZP-aPDL1 in subcutaneous lesions led to a significant reduction in tumor growth with minimal release of the antibody in the circulation. When the vector was tested in a more stringent setting (rapidly progressing peritoneal carcinomatosis), the antitumor effect was marginal even in combination with other immune-stimulatory agents such as polyinosinic-polycytidylic acid (pI:C), blocking mAbs for T cell immunoglobulin, mucin-domain containing-3 (TIM-3) or agonistic mAbs for 4-1BB (CD137). In contrast, macrophage depletion by clodronate liposomes enhanced the efficacy of HCA-EFZP-aPDL1. These results highlight the importance of addressing macrophage-associated immunoregulatory mechanisms to overcome resistance to ICIs in the context of colorectal cancer. 相似文献
62.
Ivn Benito Ignacio J. Encío Fermín I. Milagro María Alfaro Ana Martínez-Peuela Miguel Barajas Florencio Marzo 《International journal of molecular sciences》2021,22(9)
Recent studies have suggested that flavonoids such as quercetin and probiotics such as Bifidobacterium bifidum (Bf) and Lactobacillus gasseri (Lg) could play a relevant role in inhibiting colon cancer cell growth. Our study investigated the role of dietary supplementation with microencapsulated probiotics (Bf and Lg) along with quercetin in the development of mouse colorectal cancer (CRC). Methods: Adenomatous polyposis coli/multiple intestinal neoplasia (ApcMin/+) mice were fed a standard diet or the same diet supplemented with microencapsulated probiotics (Bf and Lg strains, 107 CFU/100 g food) or both probiotics strains plus microencapsulated quercetin (15 mg/100 g food) for 73 days. Changes in body and organ weights, energy metabolism, intestinal microbiota, and colon tissue were determined. The expression of genes related to the Wnt pathway was also analyzed in colon samples. Results: Dietary supplementation with microencapsulated probiotics or microencapsulated probiotics plus quercetin reduced body weight loss and intestinal bleeding in ApcMin/+ mice. An improvement in energy expenditure was observed after 8 weeks but not after 10 weeks of treatment. A supplemented diet with microencapsulated Bf and Lg reduced the number of aberrant crypt foci (ACF) and adenomas by 45% and 60%, respectively, whereas the supplementation with Bf, Lg and quercetin decreased the number of ACF and adenomas by 57% and 80%, respectively. Microencapsulated Bf and Lg in combination with quercetin could exert inhibition of the canonical Wnt/β-catenin signaling pathway in the colon of ApcMin/+ mice Conclusions: The administration of microencapsulated Bf and Lg, individually or in combination with quercetin, inhibits the CRC development in ApcMin/+ mice. 相似文献
63.
Alfredo Jurez-Saldivar Elizabeth Barbosa-Cabrera Edgar E. Lara-Ramírez Alma D. Paz-Gonzlez Ana V. Martínez-Vzquez Virgilio Bocanegra-García Isidro Palos Nuria E. Campillo Gildardo Rivera 《International journal of molecular sciences》2021,22(11)
Infectious diseases caused by intestinal protozoan, such as Entamoeba histolytica (E. histolytica) and Giardia lamblia (G. lamblia) are a worldwide public health issue. They affect more than 70 million people every year. They colonize intestines causing primarily diarrhea; nevertheless, these infections can lead to more serious complications. The treatment of choice, metronidazole, is in doubt due to adverse effects and resistance. Therefore, there is a need for new compounds against these parasites. In this work, a structure-based virtual screening of FDA-approved drugs was performed to identify compounds with antiprotozoal activity. The glycolytic enzyme triosephosphate isomerase, present in both E. histolytica and G. lamblia, was used as the drug target. The compounds with the best average docking score on both structures were selected for the in vitro evaluation. Three compounds, chlorhexidine, tolcapone, and imatinib, were capable of inhibit growth on G. lamblia trophozoites (0.05–4.935 μg/mL), while folic acid showed activity against E. histolytica (0.186 μg/mL) and G. lamblia (5.342 μg/mL). 相似文献
64.
Pablo Bonilla Joaquim Hernandez Esther Giraldo Miguel A. Gonzlez-Prez Ana Alastrue-Agudo Hoda Elkhenany María J. Vicent Xavier Navarro Michael Edel Victoria Moreno-Manzano 《International journal of molecular sciences》2021,22(11)
We currently lack effective treatments for the devastating loss of neural function associated with spinal cord injury (SCI). In this study, we evaluated a combination therapy comprising human neural stem cells derived from induced pluripotent stem cells (iPSC-NSC), human mesenchymal stem cells (MSC), and a pH-responsive polyacetal–curcumin nanoconjugate (PA-C) that allows the sustained release of curcumin. In vitro analysis demonstrated that PA-C treatment protected iPSC-NSC from oxidative damage in vitro, while MSC co-culture prevented lipopolysaccharide-induced activation of nuclear factor-κB (NF-κB) in iPSC-NSC. Then, we evaluated the combination of PA-C delivery into the intrathecal space in a rat model of contusive SCI with stem cell transplantation. While we failed to observe significant improvements in locomotor function (BBB scale) in treated animals, histological analysis revealed that PA-C-treated or PA-C and iPSC-NSC + MSC-treated animals displayed significantly smaller scars, while PA-C and iPSC-NSC + MSC treatment induced the preservation of β-III Tubulin-positive axons. iPSC-NSC + MSC transplantation fostered the preservation of motoneurons and myelinated tracts, while PA-C treatment polarized microglia into an anti-inflammatory phenotype. Overall, the combination of stem cell transplantation and PA-C treatment confers higher neuroprotective effects compared to individual treatments. 相似文献
65.
Roser Cervellera Xavier Ramis Josep Maria Salla Ana Mantecn Angels Serra 《应用聚合物科学杂志》2006,102(3):2086-2093
Diglycidyl ether of bisphenol A or 3,4‐epoxycyclohexylmethyl 3,4‐epoxycyclohexane carboxylate were mixed with different proportions of 4‐methyl‐1,3‐dioxolan‐2‐one and cured using lanthanide triflates as initiators. In order to compare the materials obtained, conventional initiators such as boron trifluoride complexes and N,N‐dimethylaminopyridine were also tested. The curing process was followed by differential scanning calorimetry (DSC) and Fourier transform IR in attenuated total reflectance mode. This technique proved that the carbonate accelerates the curing process because it helps to form the active initiating species, although it was not chemically incorporated into the network and remained entrapped in the material. The DSC kinetic study was also reported. © 2006 Wiley Periodicals Inc. J Appl Polym Sci 102: 2086–2093, 2006 相似文献
66.
Tracy Q. Gardner Ana I. Flores Richard D. Noble John L. Falconer 《American Institute of Chemical Engineers》2002,48(6):1155-1167
A transient permeation method presented here not only determines the adsorption and diffusion properties of the pores that are the transport pathways through zeolite membranes, but nondestructively estimates the effective thickness of the membrane. Transient responses of the permeate concentration to step changes in the feed were measured on two H-ZSM-5 tubular membranes and modeled assuming Maxwell-Stefan diffusion and Langmuir adsorption. The adsorption isotherms determined from these transient measurements at 298 K of N2 and CO2 were nearly identical to those measured by calorimetry on H-ZSM-5 powders. The CH4 isotherm at 298 K was similar to isotherms measured by calorimetry and gravimetric techniques on Na-ZSM-5 and silicalite powders. The similarity of the isotherms indicates that transport of these light gases occurs mainly through zeolite pores. The Maxwell-Stefan diffusion coefficients DMS depended on concentration and were higher for higher feed partial pressures. Average DMS values for the two membranes were 7.5, 5 and 1.5×10−10 m2/s for N2, CH4, and CO2, respectively; these are in the same range and order as diffusion coefficients measured in zeolite crystals. 相似文献
67.
We present a method for foreground/background video segmentation (change detection) in real-time that can be used, in applications such as background subtraction or analysis of surveillance cameras. Our approach implements a probabilistic segmentation based on the Quadratic Markov Measure Field models. This framework regularizes the likelihood of each pixel belonging to each one of the classes (background or foreground). We propose a new likelihood that takes into account two cases: the first one is when the background is static and the foreground might be static or moving (Static Background Subtraction), the second one is when the background is unstable and the foreground is moving (Unstable Background Subtraction). Moreover, our likelihood is robust to illumination changes, cast shadows and camouflage situations. We implement a parallel version of our algorithm in CUDA using a NVIDIA Graphics Processing Unit in order to fulfill real-time execution requirements. 相似文献
68.
Ana Cavalcanti Steve King Colin O’Halloran Jim Woodcock 《Formal Aspects of Computing》2014,26(4):795-823
Many tools can check if a test set provides control coverage; they are, however, of little or no help when coverage is not achieved and the test set needs to be completed. In this paper, we describe how a formal characterisation of a coverage criterion can be used to generate test data; we present a procedure based on traditional programming techniques like normalisation, and weakest precondition calculation. It is a basis for automation using an algebraic theorem prover. In the worst situation, if automation fails to produce a specific test, we are left with a specification of the compliant test sets. Many approaches to model-based testing rely on formal models of a system under test. Our work, on the other hand, is not concerned with the use of abstract models for testing, but with coverage based on the text of programs. 相似文献
69.
Dr. Christoph Goebel Prof.?Dr. Hans-Arno Jacobsen Victor del Razo Mag.? Christoph Doblander Dipl.-Ing. Jose Rivera Dipl-Inform.?Wirt Jens Ilg Dr. Christoph Flath Prof.?Dr. Hartmut Schmeck Prof.?Dr. Christof Weinhardt Dipl-Inform. Daniel Pathmaperuma Prof.?Dr. Dr.?h.?c. Hans-Jürgen Appelrath Prof.?Dr. Michael Sonnenschein Prof.?Dr. Sebastian Lehnhoff Prof.?Dr. Oliver Kramer Prof.?Dr. Thorsten Staake Prof.?Dr. Elgar Fleisch Prof.?Dr. Dirk Neumann Prof.?Dr. Jens Strüker Dr. Koray Erek Prof.?Dr. Rüdiger Zarnekow Dr. Holger Ziekow Prof.?Dr. J?rg L?ssig 《WIRTSCHAFTSINFORMATIK》2014,56(1):31-39
Due to the increasing importance of producing and consuming energy more sustainably, Energy Informatics (EI) has evolved into a thriving research area within the CS/IS community. The article attempts to characterize this young and highly dynamic field of research by describing current EI research topics and methods and provides an outlook of how the field might evolve in the future. It is shown that two general research questions have received the most attention so far and are likely to dominate the EI research agenda in the coming years: How to leverage information and communication technology (ICT) to (1) improve energy efficiency, and (2) to integrate decentralized renewable energy sources into the power grid. Selected EI streams are reviewed, highlighting how the respective research questions are broken down into specific research projects and how EI researchers have made contributions based on their individual academic background. 相似文献
70.
Naoufal Bahlawane Edgar Fischer Rivera Katharina Kohse-Hinghaus Armin Brechling Ulf Kleineberg 《Applied catalysis. B, Environmental》2004,53(4):245-255
The chemical vapor deposition method was used to deposit thin films of cobalt oxide starting with cobalt (II) acetylacetonate and oxygen. The deposition process was investigated and the obtained films were identified as a cubic spinel-type polycrystalline Co3O4 with a crystallite size of 30–40 nm. The coating was carbon-free and the surface oxygen concentration was measured to be 66 at.% with AES analysis. Smooth and highly uniform thin films were deposited on planar stainless steel substrates and subjected to TPR and catalysis tests that show positive correlation. The apparent activation energy of Co3O4 reduction to CoO was measured to be (33±5) kJ/mol. The catalytic activity of Co3O4 was investigated toward the conversion of both propane and ethanol to carbon dioxide. Though the catalytic action was registered at the same temperature, the deactivation process was seen to be different. The catalytic conversion of ethanol induces a fast deactivation process, which was linked to its high ability to reduce Co3O4. 相似文献