Cellular and subcellular localization of transforming growth factor-alpha and epidermal growth factor receptor in normal and diseased human and hamster pancreas

Four normal pancreas, 8 chronic pancreatitis specimens, and 30 non-endocrine pancreatic tumors from humans and 6 normal and 6 induced pancreatic cancers in hamsters were examined immunohistochemically by antibodies against human transforming growth factor-alpha (TGF-alpha) and epidermal growth factor receptor (EGFR). Two normal pancreas and two pancreatic cancer specimens from each species were also studied immunoelectron microscopically by the immunogold method. In chronic pancreatitis, the reactivity and intensity of the staining with both antibodies were much greater in ductal/ductular cells than in the normal pancreas. All 30 pancreatic cancers reacted with both antibodies with a variable degree of reactivity and staining intensity. No correlation was found between the histological type of tumors, the degree of tumor differentiation, and the incidence and patterns of reactivity of either antibody. Immunoelectron microscopically, both EGFR and TGF-alpha were demonstrated primarily on the basal membrane. In the normal hamster pancreas, TGF-alpha was overexpressed in the alpha-cells but not in any other islet cells. Both TGF-alpha and EGFR were marginally detectable in the exocrine pancreas and in induced pancreatic lesions. This is the first demonstration of subcellular localization of TGF-alpha and EGFR in the normal and diseased human and hamster pancreas.     

Design of an isolated pig heart preparation for positron emission tomography and magnetic resonance imaging

RATIONALE AND OBJECTIVES: Validation of new positron emission tomography (PET) tracers or magnetic (MR) imaging contrast agents is based on isolated rodent heart preparations. The use of larger animals could provide a more direct validation using the devices used for humans. METHODS: An isolated pig heart preparation has been developed and adapted to the technical constraints of whole body PET and MR imaging. This preparation could be used either in the Langendorff or working mode after selective cannulation of both coronary arteries. RESULTS: The authors showed that quantification of regional kinetics of PET tracers was possible using this preparation by measuring fluorine-18-labeled deoxyglycose (18FDG) kinetics in remote and ischemic territories. Experiments using MR imaging contrast agents, for myocardial perfusion, demonstrated the ability of this preparation to accurately validate these contrast agents over a wide range of flow rates. CONCLUSIONS: An isolated pig heart preparation could be developed to fulfill the constraints of PET and MR imaging, and proved useful for the study of the distribution of different tracers or contrast media developed for functional cardiac imaging in humans.     

Short cue presentations encourage advance task preparation: A recipe to diminish the residual switch cost.

In the task-switching literature, it has frequently been demonstrated that although advance task preparation reduces the switch cost, it never really eliminates the switch cost. This remaining residual switch cost received much attention, and it has been argued that advance preparation is restricted in nature. In the present study, the role of task-cue presentation in the establishment of the residual switch cost was investigated. In 4 experiments, the cue was removed during the preparation interval, and it was hypothesized that this would encourage participants to complete advance task preparation. The results of all 4 experiments provided support for this hypothesis: When the cue was presented for a short time and then removed, the residual switch cost completely disappeared. This was found for different cue types. Furthermore, Experiment 3 demonstrated that it was not the presence of the cue itself but merely differences in advance task preparation that caused the effects. This suggests that advance task preparation is not as restricted in nature as previously assumed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Prevalence of serologic markers of HBV, HDV, HCV and HIV in non-injection drug users compared to injection drug users in Gran Canaria, Spain

Von Hippel-Lindau disease (VHL) is an autosomal dominant tumour syndrome caused by germline mutations of the VHL tumour suppressor gene located on chromosome 3p25-26. In VHL tumours may occur in 14 different target organs, including the eye. Retinal angiomas are considered the first manifestation of VHL disease in 43% of cases, and the cumulative probability of developing a retinal angioma in one or both eyes rises during each decade of life, reaching 80% for patients over 80 years old. Since 1976 patients with VHL at the University Hospital of Utrecht and their at-risk relatives have been screened periodically by a multidisciplinary team. Long-term follow-up ophthalmological data were analysed with special attention to natural course and results of treatment. In addition, we looked for a genotype-phenotype correlation. Retinal angiomas were found in all families. In one large family with a missense mutation (V170D) of the VHL gene, in which the complete spectrum of visceral- and central nervous system (CNS) features of VHL is present, macular, parapapillary, optic disc and ora serrata angiomas were also found. In general, however, a clear-cut genotype-phenotype correlation could not be found. Only early detection and treatment of peripheral retinal angiomas can be expected to decrease the percentage of patients with decreased visual acuity. Therefore, early detection and treatment of these tumours is of paramount importance. Ophthalmological screening of patients and persons at risk should start as early as possible. In patients with apparently sporadic retinal angiomas it is advisable to perform germline DNA analysis, since the risk of developing VHL is high, especially if the angiomas are bilateral, or unilateral and multifocal, if the patient is young, or if there is a family history suggestive of VHL.     

Performance and energy task migration model for heterogeneous clusters

The Journal of Supercomputing - This article presents a set of linear regression models to predict the impact of task migration on different objectives, like performance and energy consumption. It...     

Deploying deep learning approaches to left ventricular non-compaction measurement

The Journal of Supercomputing - Left ventricular non-compaction (LVNC) is a rare cardiomyopathy characterized by abnormal trabeculations in the left ventricle cavity. Although traditional computer...     

A neuron membrane mesh representation for visualization of electrophysiological simulations

We present a process to automatically generate three-dimensional mesh representations of the complex, arborized cell membrane surface of cortical neurons (the principal information processing cells of the brain) from nonuniform morphological measurements. Starting from manually sampled morphological points (3D points and diameters) from neurons in a brain slice preparation, we construct a polygonal mesh representation that realistically represents the continuous membrane surface, closely matching the original experimental data. A mapping between the original morphological points and the newly generated mesh enables simulations of electrophysiolgical activity to be visualized on this new membrane representation. We compare the new mesh representation with the state of the art and present a series of use cases and applications of this technique to visualize simulations of single neurons and networks of multiple neurons.     

A fuzzy queue-aware routing approach for wireless mesh networks

Recent advances in Wireless Mesh Networks (WMNs) have overcome the drawbacks of traditional wired and ad-hoc networks and now they are seen as a means of allowing last mile communications with quality level assurance in Future Multimedia Systems. However, new routing schemes are needed to provide end-to-end Quality of Service (QoS) and Quality of Experience (QoE) support for delay/loss/jitter-sensitive multimedia applications. The well-known OLSR (Optimized Link State Routing) protocol with ETX (Expected Transmission Count) metric brings many benefits to the path selection process, but has a drawback with regard to queue availability management, which reduces the system performance. This problem is caused when OLSR-EXT control messages are exchanged and the queues of mesh routers along the end-to-end communication path are overloaded. As a result, multimedia-related packets will suffer from loss/delay/jitter and the overall system performance will decrease. This paper proposes the Optimized Link State Routing-Fuzzy ETX Queue (OLSR-FEQ) protocol to overcome the limitations of OLSR-ETX regarding queue availability, QoS and QoE assurance. OLSR-FEQ optimizes network and user-based parameters by coordinating queue availability, QoS and fuzzy issues in the routing decision process as a way of allocating the best paths for multimedia applications. Performance evaluations were carried out with the Network Simulator (NS-2.34) to show the benefits of the proposed solution when compared with existing routing schemes, namely OLSR-ETX, OLSR-FLC, OLSR-MD and HWMP (IEEE 802.11s standard), regarding QoS (unsuccessful packet delivery and throughput) and QoE (PSNR, SSIM, VQM and MOS) parameters.