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Lorenc-Kubis I Kowalska J Pochroń B Zuzło A Wilusz T 《Chembiochem : a European journal of chemical biology》2001,2(1):45-51
LUTI (Linum usitatissimum trypsin inhibitor), a member of the potato inhibitor I family, has been isolated from seeds of flax by ethanol fractionation, ion exchange chromatography on CM-Sephadex C-25, affinity purification on immobilized methylchymotrypsin (alpha-chymotrypsin in which His57 has been converted to 3-methylhistidine) in the presence of 5M NaCl, and finally by reversed-phase HPLC. The 7655 Da inhibitor consists of a single polypeptide chain of 69 residues with one disulfide bridge. The molecule is acetylated at the N terminus. Its primary structure has been determined after limited proteolysis of the native molecule with trypsin at the reactive site, cleavage with cyanogen bromide or arginyl endopeptidase (Arg-gingipain), and alcoholytic deacetylation of the N-terminally blocked serine. The association constants (K(a)) of LUTI with bovine beta-trypsin and alpha-chymotrypsin are 3.58x10(10) M(-1) and 5.02x10(5) M(-1), respectively. High NaCl concentration (3M) increased the association constant of LUTI with alpha-chymotrypsin to 6.64x10(7) M(-1). To our knowledge, LUTI is the first serine-proteinase-type inhibitor isolated from a plant of the Linaceae family. 相似文献
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The two types of high-sulphur coals Mequinenza and Illinois No. 6, in the initial form and subjected to potassium/liquid ammonia reduction, were analysed by atmospheric pressure-temperature programmed reduction (AP-TPR) method. It has been shown that preliminary demineralisation was beneficial for AP-TPR measurements because of the removal of calcium compounds. The reduction of sulphides and disulphides in the potassium/liquid ammonia system was found to lead to formation of aromatic and aliphatic thiols. The presence of the latter is better manifested in the AP-TPR kinetograms when the measurements are performed in the presence of a special reducing mixture. It has been shown that the coal reduction in the potassium/liquid ammonia system apart from transformations of non-thiophene sulphur groups also leads to breaking up of the C-S bonds in some thiophene systems. 相似文献
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The chemical composition, defect structure, and diffusion in nickel sulfide -Ni3S2 have been investigated in H2S-H2 mixtures containing between 1 and 65% H2S between 560 and 700°C. Gravimetric, density, and X-ray studies were carried out. In the thermodynamically stable compound the ratio of Ni/S varied between 1.3 and 1.75. The X-ray examination showed a step change in the lattice parameter at the Ni/S ratio 1.4. A linear dependence of the density values (between 5.5 and 6.2 g/cm3) on the composition was observed. On the basis of the chemical composition and density measurements the number of nickel and sulfur atoms in 1 cm3 were determined. It has been shown that the Ni3S2 phase is defected in both anion and cation sublattices and that its chemical formula may be described as follows: Ni3±yS2x, where y 2x. It has been found that in the mixture containing 10% H2S the process of defect formation is determined by their diffusion in the sulfide. The temperature dependence of the diffusion coefficient is described by the equation D = 13.15 exp(-30,000/RT) cm2/sec. No dependence of D on the sulfur partial pressure was observed, but this may be due to the relatively large uncertainties in the measurement of the diffusion coefficients. 相似文献
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The kinetics of copper sulfidation have been studied as a function of temperature (570–1120 K) by the use of the modified Wagner's pellet method. It has been found that sulfidation follows a parabolic rate law with different activation energies in the low- and high-temperature ranges: $$k_{p}^{'} = 10.2 exp - (70 \pm 4)[kJ/mole]/RT (570 - 780 K)$$ and $$k_{p}^{'} = 1.69 \times 10^{ - 3} exp - (13 \pm 1.0)[kJ/mole]/RT (780 - 1120 K)$$ Using thermodynamic data as well as interfacial chemical potentials and ionic conductivity, the parabolic rate constants of sulfidation of copper have been calculated with good agreement with experimental results. Parabolic rate constants of sulfidation have been used to calculate average self-diffusion coefficients of copper in Cu2S, the temperature dependence of which is as follows: $$\bar D_{Cu} = 8.76 \times 10^{ - 4} exp - (22.4 \pm 1)[kJ/mole]/RT$$ 相似文献
86.
Hypersequent calculi, introduced independently by Pottinger and Avron, provide a powerful generalization of ordinary sequent calculi. In the paper we present a proof of eliminability of cut in hypersequent calculi for three modal logics of linear frames: K4.3, KD4.3 and S4.3. Our cut-free calculus is based on Avron's HC formalization for Gödel–Dummett's logic. The presented proof of eliminability of cut is purely syntactical and based on Ciabattoni, Metcalfe, Montagna's proof of eliminability of cut for hypersequent calculi for some fuzzy logics with modalities. 相似文献
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A two‐level detector of short‐term unique changes in time series based on a similarity method 下载免费PDF全文
In the paper, a novel two‐level algorithm of time‐series change detection is presented. In the first level, to identify non‐stationary sequences in a processed signal, preliminary detection of events is performed with a short‐term prediction comparison. In the second stage, to confirm the changes detected in the first level, a similarity method aimed at identification of unique changes is employed. The detection of changes in a non‐stationary time series is discussed, implemented algorithms are described and the results produced on a sample four financial time series are shown. General conditions for implementing the proposed algorithm as an immune‐like event detector are discussed. 相似文献
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