全文获取类型
收费全文 | 2609篇 |
免费 | 118篇 |
国内免费 | 21篇 |
专业分类
电工技术 | 39篇 |
综合类 | 18篇 |
化学工业 | 683篇 |
金属工艺 | 57篇 |
机械仪表 | 47篇 |
建筑科学 | 81篇 |
矿业工程 | 7篇 |
能源动力 | 74篇 |
轻工业 | 328篇 |
水利工程 | 28篇 |
石油天然气 | 27篇 |
武器工业 | 2篇 |
无线电 | 253篇 |
一般工业技术 | 307篇 |
冶金工业 | 494篇 |
原子能技术 | 18篇 |
自动化技术 | 285篇 |
出版年
2023年 | 15篇 |
2022年 | 82篇 |
2021年 | 110篇 |
2020年 | 62篇 |
2019年 | 46篇 |
2018年 | 66篇 |
2017年 | 62篇 |
2016年 | 57篇 |
2015年 | 68篇 |
2014年 | 80篇 |
2013年 | 135篇 |
2012年 | 104篇 |
2011年 | 132篇 |
2010年 | 98篇 |
2009年 | 127篇 |
2008年 | 105篇 |
2007年 | 127篇 |
2006年 | 91篇 |
2005年 | 74篇 |
2004年 | 69篇 |
2003年 | 84篇 |
2002年 | 75篇 |
2001年 | 52篇 |
2000年 | 41篇 |
1999年 | 32篇 |
1998年 | 172篇 |
1997年 | 108篇 |
1996年 | 101篇 |
1995年 | 58篇 |
1994年 | 42篇 |
1993年 | 49篇 |
1992年 | 18篇 |
1991年 | 17篇 |
1990年 | 15篇 |
1989年 | 13篇 |
1988年 | 8篇 |
1987年 | 14篇 |
1986年 | 13篇 |
1985年 | 13篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 4篇 |
1981年 | 7篇 |
1980年 | 8篇 |
1979年 | 11篇 |
1978年 | 7篇 |
1977年 | 12篇 |
1976年 | 22篇 |
1973年 | 3篇 |
1971年 | 7篇 |
排序方式: 共有2748条查询结果,搜索用时 15 毫秒
61.
Influence of sepiolite nanoclay on the properties of the resulting join between poly(lactic acid) (PLA) and different Polyamide66 (PA66)/nanoclay nanocomposites was studied in this work. Six different polymer nanocomposites based on PA66 were manufactured through a melt compounding process by adding a fixed 1.64 wt % of a commercial IR absorber additive and the respective weight percentages of sepiolite to the polymer matrix. Several nanocomposite/PLA joints were finally performed by means of the transmission laser welding technology and the resulting weldings were characterized in terms of mechanical properties by performing peeling and shearing tests. Furthermore, both welded and mechanically tested samples were also analyzed by scanning electron microscopy in order to study the morphology of the weld seam. The results of the performed tests show that the addition of sepiolite to the PA66 improves the welding performance only in those cases in which the percentage of sepiolite of the nanocomposites is higher than 5 wt %. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018 , 135, 46638. 相似文献
62.
Immacolata Ruggiero Monica Terracciano Nicola M Martucci Luca De Stefano Nunzia Migliaccio Rosarita Tatè Ivo Rendina Paolo Arcari Annalisa Lamberti Ilaria Rea 《Nanoscale research letters》2014,9(1):329
Diatomite is a natural fossil material of sedimentary origin, constituted by fragments of diatom siliceous skeletons. In this preliminary work, the properties of diatomite nanoparticles as potential system for the delivery of drugs in cancer cells were exploited. A purification procedure, based on thermal treatments in strong acid solutions, was used to remove inorganic and organic impurities from diatomite and to make them a safe material for medical applications. The micrometric diatomite powder was reduced in nanoparticles by mechanical crushing, sonication, and filtering. Morphological analysis performed by dynamic light scattering and transmission electron microscopy reveals a particles size included between 100 and 300 nm. Diatomite nanoparticles were functionalized by 3-aminopropyltriethoxysilane and labeled by tetramethylrhodamine isothiocyanate. Different concentrations of chemically modified nanoparticles were incubated with cancer cells and confocal microscopy was performed. Imaging analysis showed an efficient cellular uptake and homogeneous distribution of nanoparticles in cytoplasm and nucleus, thus suggesting their potentiality as nanocarriers for drug delivery.
PACS
87.85.J81.05.Rm; 61.46. + w 相似文献63.
Synthetic and Biological Studies of Sesquiterpene Polygodial: Activity of 9‐Epipolygodial against Drug‐Resistant Cancer Cells 下载免费PDF全文
Dr. Ramesh Dasari Annelise De Carvalho Derek C. Medellin Kelsey N. Middleton Dr. Frédéric Hague Marie N. M. Volmar Prof. Liliya V. Frolova Dr. Mateus F. Rossato Jorge J. De La Chapa Nicholas F. Dybdal‐Hargreaves Akshita Pillai Prof. Véronique Mathieu Prof. Snezna Rogelj Prof. Cara B. Gonzales Prof. João B. Calixto Prof. Antonio Evidente Dr. Mathieu Gautier Dr. Gnanasekar Munirathinam Prof. Rainer Glass Prof. Patricia Burth Dr. Stephen C. Pelly Prof. Willem A. L. van Otterlo Prof. Robert Kiss Prof. Alexander Kornienko 《ChemMedChem》2015,10(12):2014-2026
Polygodial, a terpenoid dialdehyde isolated from Polygonum hydropiper L., is a known agonist of the transient receptor potential vanilloid 1 (TRPV1). In this investigation a series of polygodial analogues were prepared and investigated for TRPV1‐agonist and anticancer activities. These experiments led to the identification of 9‐epipolygodial, which has antiproliferative potency significantly exceeding that of polygodial. 9‐Epipolygodial was found to maintain potency against apoptosis‐resistant cancer cells as well as those displaying the multidrug‐resistant (MDR) phenotype. In addition, the chemical feasibility for the previously proposed mechanism of action of polygodial, involving the formation of a Paal–Knorr pyrrole with a lysine residue on the target protein, was demonstrated by the synthesis of a stable polygodial pyrrole derivative. These studies reveal rich chemical and biological properties associated with polygodial and its direct derivatives. These compounds should inspire further work in this area aimed at the development of new pharmacological agents, or the exploration of novel mechanisms of covalent modification of biological molecules with natural products. 相似文献
64.
Ergothioneine Biosynthetic Methyltransferase EgtD Reveals the Structural Basis of Aromatic Amino Acid Betaine Biosynthesis 下载免费PDF全文
Allegra Vit Laëtitia Misson Prof. Dr. Wulf Blankenfeldt Prof. Dr. Florian P. Seebeck 《Chembiochem : a European journal of chemical biology》2015,16(1):119-125
Ergothioneine is an N‐α‐trimethyl‐2‐thiohistidine derivative that occurs in human, plant, fungal, and bacterial cells. Biosynthesis of this redox‐active betaine starts with trimethylation of the α‐amino group of histidine. The three consecutive methyl transfers are catalyzed by the S‐adenosylmethionine‐dependent methyltransferase EgtD. Three crystal structures of this enzyme in the absence and in the presence of N‐α‐dimethylhistidine and S‐adenosylhomocysteine implicate a preorganized array of hydrophilic interactions as the determinants for substrate specificity and apparent processivity. We identified two active site mutations that change the substrate specificity of EgtD 107‐fold and transform the histidine‐methyltransferase into a proficient tryptophan‐methyltransferase. Finally, a genomic search for EgtD homologues in fungal genomes revealed tyrosine and tryptophan trimethylation activity as a frequent trait in ascomycetous and basidomycetous fungi. 相似文献
65.
66.
María García-álvaro Annalisa Addante Cesáreo Roncero Margarita Fernández Isabel Fabregat Aránzazu Sánchez Blanca Herrera 《International journal of molecular sciences》2015,16(9):20431-20448
The study of bone morphogenetic proteins (BMPs) role in tumorigenic processes, and specifically in the liver, has gathered importance in the last few years. Previous studies have shown that BMP9 is overexpressed in about 40% of hepatocellular carcinoma (HCC) patients. In vitro data have also shown evidence that BMP9 has a pro-tumorigenic action, not only by inducing epithelial to mesenchymal transition (EMT) and migration, but also by promoting proliferation and survival in liver cancer cells. However, the precise mechanisms driving these effects have not yet been established. In the present work, we deepened our studies into the intracellular mechanisms implicated in the BMP9 proliferative and pro-survival effect on liver tumor cells. In HepG2 cells, BMP9 induces both Smad and non-Smad signaling cascades, specifically PI3K/AKT and p38MAPK. However, only the p38MAPK pathway contributes to the BMP9 growth-promoting effect on these cells. Using genetic and pharmacological approaches, we demonstrate that p38MAPK activation, although dispensable for the BMP9 proliferative activity, is required for the BMP9 protective effect on serum withdrawal-induced apoptosis. These findings contribute to a better understanding of the signaling pathways involved in the BMP9 pro-tumorigenic role in liver tumor cells. 相似文献
67.
Yaofeng Li Xiangyun Chen Xiaofang Tang Chundong Zhang La Wang Peng Chen Minhui Pan Cheng Lu 《International journal of molecular sciences》2015,16(3):6266-6280
Silk gland cells undergo multiple endomitotic cell cycles during silkworm larval ontogeny. Our previous study demonstrated that feeding is required for continued endomitosis in the silk gland cells of silkworm larvae. Furthermore, the insulin signaling pathway is closely related to nutritional signals. To investigate whether the insulin signaling pathway is involved in endomitosis in silk gland cells, in this study, we initially analyzed the effects of bovine insulin on DNA synthesis in endomitotic silk gland cells using 5-bromo-2''-deoxyuridine (BrdU) labeling technology, and found that bovine insulin can stimulate DNA synthesis. Insulin signal transduction is mainly mediated via phosphoinositide 3-kinase (PI3K)/Akt, the target of rapamycin (TOR) and the extracellular signal-regulated kinase (ERK) pathways in vertebrates. We ascertained that these three pathways are involved in DNA synthesis in endomitotic silk gland cells using specific inhibitors against each pathway. Moreover, we investigated whether these three pathways are involved in insulin-stimulated DNA synthesis in endomitotic silk gland cells, and found that the PI3K/Akt and TOR pathways, but not the ERK pathway, are involved in this process. These results provide an important theoretical foundation for the further investigations of the mechanism underlying efficient endomitosis in silk gland cells. 相似文献
68.
The development of Generation IV sodium-cooled fast reactors (SFR) is currently studied by several countries, France in particular. To manufacture the UO2-PuO2 fuels for these new types of reactors, new innovative wet colloidal processing routes are investigated. Among these wet colloidal processes, some involve at first the preparation of high solid content water-based suspensions. This key step needs to be investigated in order to obtain highly and easily processable suspensions, featuring optimal viscosity and dispersion state. The structures and properties for all intermediate and final products involved in such ceramic manufacturing processes are heavily affected by these suspension characteristics. Therefore, they are critical to ensure a compliant final product (i.e. fuel pellets) with the required density, homogeneity, mechanical strength and absence of defects. In this scope, preparation process of such suspensions was developed by the use of UO2 and PuO2 surrogating (i.e. mimicking) powders, TiO2 and Y2O3 respectively. 相似文献
69.
Mauro F. La Russa Silvestro A. Ruffolo Natalia Rovella Cristina M. Belfiore Anna M. Palermo Maria T. Guzzi Gino M. Crisci 《Progress in Organic Coatings》2012
Environmental pollution arising from industrial implants and urban factors is constantly increasing, causing aesthetical and durability concerns to urban structures exposed to the atmosphere. 相似文献
70.
Dario Imbraguglio Andrea Mario Giovannozzi Annalisa Nastro Andrea Mario Rossi 《Nanoscale research letters》2012,7(1):530
Three-dimensional submicrometric structures and biomolecular patterns have been fabricated on a porous silicon film by an electron beam-based functionalization method. The immobilized proteins act as a passivation layer against material corrosion in aqueous solutions. The effects'' dependence on the main parameters of the process (i.e., the electron beam dose, the biomolecule concentration, and the incubation time) has been demonstrated. 相似文献