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Maud-Alix Mader Chaouqi Misbah Thomas Podgorski 《Microgravity science and technology》2006,18(3-4):200-203
Vesicles are closed fluid membranes with a size in the range 10–100 µm. Under flow they exhibit various dynamics such as tank-treading, tumbling, and vacillating-breathing. Part of these dynamics are studied experimentally both on earth and under microgravity conditions. We report on the basic results of vesicles dynamics. Deviations from existing theories are discussed. A small deformation theory is presented that shows notable differences from those imposing a fixed shape: the vesicle shape is shown to undergo a temporal oscillation. This theory allows one to extract the effective viscosity of the suspension. 相似文献
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Irina Mader Werner Roser Gisela Hagberg Monika Schneider Rolf Sauter Joachim Seelig Ernst W. Radue Wolfgang Steinbrich 《Magma (New York, N.Y.)》1996,4(2):139-150
Seventeen patients with presumed glial brain tumors were examined with proton chemical shift imaging and single voxel spectroscopy that used different echo times. Metabolite resonances were evaluated by metabolic ratios and absolutely by correcting for coil load and comparison to phantom measurements. Metabolic images were created to visualize the metabolic changes. All patients showed spectra that were different from those measured in healthy control subjects. Spectral changes were also present in normal-appearing matter (NAM) that was distant from lesions. The resonance at 3.55 ppm which is usually assigned to bothmyo-inositol and glycine, was the only one to allow a discrimination between healthy volunteers, astrocytoma grade II, and glioblastoma multiforme (GBM) (p<0.02). From the different echo times used we conclude that an increase inhis resonance has to be assigned to glycine rather thanmyo-inositol. This resonance might be used to grade human gliomas more reliably. Total creatine (Cr) decreased more drastically with malignancy than N-acetylated metabolites (NA). This led to a higher NA/Cr ratio in GBM compared to astrocytoma grade II. NA/Cr was thus pseudonormal in GBM due to a change in both nominator and denominator. This study reveals the importance of comparing magnetic resonance spectroscopy data of lesions to spectra measured in identical localizations in healthy control subjects instead of NAM and the importance of quantifying single metabolic peaks instead of creating metabolic ratios in clinical magnetic resonance spectroscopy. 相似文献
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Tereza Cabicarová Eva Kaclíková Anneluise Mader Jana Minarovičová Janka Koreňová Tomáš Kuchta 《Food Analytical Methods》2016,9(7):1980-1984
Various variants of ISO 6888-1:1999 and ISO 6888-3:2003 methods for the detection of Staphylococcus aureus were evaluated and improved for their application to conduct analysis in spices and herbs. Improvement substantiated in washing of the sample to remove compounds interfering with the analysis and in the use of PCR for final detection, instead of plating on Baird-Parker agar, to improve sensitivity at high backgrounds and to save time. The evaluation of the method variants was based on determination of the detection limit (LOD) using series of artificially contaminated spices (allspice, black pepper, cinnamon, nutmeg, paprika, vanilla) and herbs (basil, oregano, parsley, thyme). The method without enrichment, ISO 6888-1:1999, produced LODs of 103–105 CFU/g with no positive effect of washing the sample or use of PCR for final detection. The method with enrichment, ISO 6888-3:2003, had LOD of 100 CFU/g for basil, black pepper, paprika and parsley. If the washing step was added and PCR was used for final detection, LOD of 100 CFU/g was determined also for cinnamon, nutmeg and vanilla, and LOD of 101 CFU/g was determined for allspice. For oregano and thyme, which strongly inhibit the growth of S. aureus, an alternative enrichment-independent method based on direct DNA extraction coupled to real-time PCR may be advantageous. 相似文献
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An important aspect of the regulation of gene expression is the modulation of translation rates in response to growth factors, hormones and mitogens. Most of this control is at the level of translation initiation. Recent studies have implicated the MAP kinase pathway in the regulation of translation by insulin and growth factors. MAP kinase phosphorylates a repressor of translation initiation [4E-binding protein (BP) 1] that binds to the mRNA 5' cap binding protein eukaryotic initiation factor (eIF)-4E and inhibits cap-dependent translation. Phosphorylation of the repressor decreases its affinity for eIF-4E, and thus relieves translational inhibition. eIF-4E forms a complex with two other polypeptides, eIF-4A and p220, that promote 40S ribosome binding to mRNA. Here, we have studied the mechanism by which 4E-BP1 inhibits translation. We show that 4E-BP1 inhibits 48S pre-initiation complex formation. Furthermore, we demonstrate that 4E-BP1 competes with p220 for binding to eIF-4E. Mutants of 4E-BP1 that are deficient in their binding to eIF-4E do not inhibit the interaction between p220 and eIF-4E, and do not repress translation. Thus, translational control by growth factors, insulin and mitogens is affected by changes in the relative affinities of 4E-BP1 and p220 for eIF-4E. 相似文献
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S. Mader 《Journal of Electronic Materials》1980,9(6):963-976
After drive-in of arsenic-implanted emitter structures dislocations are found at the edge of the masking window and in the
unimplanted surrounding regions while the implanted areas are defect-free. These dislocations originate from a defect structure
which exists temporarily in the emitters during drive-in heat treatment. The dislocations are attracted to the window edge
and are pushed into the surrounding region by mechanical stresses which have their origin in the intrinsic stress of the masking
layer. No penetration of dislocations outside the implanted area is observed when the implantation is done through a thin
oxide film. A mechanical stress analysis of film edge induced stresses can account for the observed defect patterns. 相似文献
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