Analysis of consumer-oriented quality characteristics of raw and boiled plantains in Cameroon: implication for breeding

This study aimed at understanding users’ demand for raw and boiled plantains in rural and urban areas in West and Littoral regions of Cameroon. Surveys conducted in eight rural localities consisted of key informant interviews, gender-disaggregated focus group discussions, market and individual interviews. Processing and cooking diagnoses were done with restaurant cooks in urban areas, to know the details of plantain processing and boiling and to understand the quality characteristics of raw plantain that give a most-liked boiled plantain. Local favourite landraces, most cultivated landraces in Cameroon (Batard and Big ebanga) and a new CARBAP/CIRAD hybrid (CARBAP K74) were used. The preference for plantain cultivars was both gender and region-dependent. High-quality plantain should be mature, with big fingers and having a dark green peel colour. The fruit length and girth, pulp pH, dry matter content and firmness were found to be relevant postharvest quality characteristics for plantain breeding improvement.     

Indoor fine particulate matter and demographic,household, and wood stove characteristics among rural US homes heated with wood fuel

Household heating using wood stoves is common practice in many rural areas of the United States (US) and can lead to elevated concentrations of indoor fine particulate matter (PM_2.5). We collected 6-day measures of indoor PM_2.5 during the winter and evaluated household and stove-use characteristics in homes at three rural and diverse study sites. The median indoor PM_2.5 concentration across all homes was 19 µg/m³, with higher concentrations in Alaska (median = 30, minimum = 4, maximum = 200, n = 10) and Navajo Nation homes (median = 29, minimum = 3, maximum = 105, n = 23) compared with Montana homes (median = 16, minimum = 2, maximum = 139, n = 59). Households that had not cleaned the chimney within the past year had 65% higher geometric mean PM_2.5 compared to those with chimney cleaned within 6 months (95% confidence interval [CI]: −1, 170). Based on a novel wood stove grading method, homes with low-quality and medium-quality stoves had substantially higher PM_2.5 compared to homes with higher-quality stoves (186% higher [95% CI: 32, 519] and 161% higher; [95% CI:27, 434], respectively). Our findings highlight the need for, and complex nature of, regionally appropriate interventions to reduce indoor air pollution in rural wood-burning regions. Higher-quality stoves and behavioral practices such as regular chimney cleaning may help improve indoor air quality in such homes.     

High Aspect Ratio Sub‐Micrometer Channels Using Wet Etching: Application to the Dynamics of Red Blood Cell Transiting through Biomimetic Splenic Slits

Nanoparticles delivering drugs, disseminating cancer cells, and red blood cells (RBCs) during splenic filtration must deform and pass through the sub‐micrometer and high aspect ratio interstices between the endothelial cells lining blood vessels. The dynamics of passage of particles/cells through these slit‐like interstices remain poorly understood because the in vitro reproduction of slits with physiological dimensions in devices compatible with optical microscopy observations requires expensive technologies. Here, novel microfluidic PDMS devices containing high aspect ratio slits with sub‐micrometer width are molded on silicon masters using a simple, inexpensive, and highly flexible method combining standard UV lithography and anisotropic wet etching. These devices enabled revealing novel modes of deformations of healthy and diseased RBCs squeezing through splenic‐like slits (0.6–2 × 5–10 × 1.6–11 µm³) under physiological interstitial pressures. At the slit exit, the cytoskeleton of spherocytic RBCs seemed to be detached from the lipid membrane whereas RBCs from healthy donors and patients with sickle cell disease exhibited peculiar tips at their front. These tips disappeared much slower in patients' cells, allowing estimating a threefold increase in RBC cytoplasmic viscosity in sickle cell disease. Measurements of time and rate of RBC sequestration in the slits allowed quantifying the massive trapping of spherocytic RBCs.     

Development of a Sensitive Microarray Platform for the Ranking of Galectin Inhibitors: Identification of a Selective Galectin‐3 Inhibitor

Glycan microarrays are useful tools for lectin glycan profiling. The use of a glycan microarray based on evanescent‐field fluorescence detection was herein further extended to the screening of lectin inhibitors in competitive experiments. The efficacy of this approach was tested with 2/3′‐mono‐ and 2,3′‐diaromatic type II lactosamine derivatives and galectins as targets and was validated by comparison with fluorescence anisotropy proposed as an orthogonal protein interaction measurement technique. We showed that subtle differences in the architecture of the inhibitor could be sensed that pointed out the preference of galectin‐3 for 2′‐arylamido derivatives over ureas, thioureas, and amines and that of galectin‐7 for derivatives bearing an α substituent at the anomeric position of glucosamine. We eventually identified a diaromatic oxazoline as a highly specific inhibitor of galectin‐3 versus galectin‐1 and galectin‐7.     

Comparison of intensive versus moderate chemotherapy of lymphocytic lymphomas: a progress report

In an Easter Cooperative Oncology Group trial, Cytoxan-prednisone (CP) Induction was compared to BCNU-prednisone (BP) in 273 patients with lymphocytic lymphoma. Response rates were comparable, with 21% achieving complete response and 40%, partial response. Patients with a nodular pattern responded better. Maintenance phase comparing cyclic intensive therapy (BCVP) with intermittent chlorambucil revealed the superiority of BCVP as demonstrated by improvement of the quality of response and somewhat longer remissions. The value of the Rappaport classification in the evaluation of lymphoma chemotherapy results is discussed. It is suggested tha NHL be separated into "favorable" and "unfavorable" groups, based on the presence or absence of nodularity and treatment schedules devised accordingly.     

Transition Between University Students to Teachers: Practice in the Middle

Abstract

This article presents a study done in an elementary mathematics methods course that focused on the transition of novice teachers’ epistemological stances: former elementary student, university student, and teacher stances. In order to help them develop the teacher stance, we designed a three-phase activity, where two phases took place inside class and the last one occurred outside of class. Novice teachers were given an assignment where they had to rehearse a count in class and enact it in front of a small group of students. They had to write reflections on their rehearsal and enactment. Interviews were done 4 months after the end of the course. The results show that the reflections about mathematics in relation to the use of new teaching practices on eliciting students’ thinking allowed the novice teachers to develop the teacher stance.     

Building a geodatabase for mapping hydrogeological features and 3D modeling of groundwater systems: Application to the Saguenay-Lac-St.-Jean region, Canada

Understanding and managing groundwater resources require the integration of a large amount of high-quality data from a variety of sources. Due to the limitations in accessing information related to groundwater and subsurface conditions, the gathering of available existing information is of crucial importance when conducting a successful hydrogeological study. Here, we present an approach for the development of an exhaustive and comprehensive groundwater database through (1) the gathering of relevant sources of information relating to groundwater, and (2) the application of a quality control process in order to screen the data for accuracy and quality. This hydrogeological database is then implemented within a GIS (geographic information system) framework coupled to a Relational Database Management System (RDBMS) as a personal geodatabase (ESRI format) GIS technology. Once established, the spatial database allows a user to request the relevant data required for a specific hydrogeological study. In addition, stratigraphic data stored within the spatial database may be utilized for constructing 3D subsurface hydrostructural models. In order to achieve this objective, the software Arc Hydro Groundwater combined with the ArcGIS spatial database is shown to be appropriate for the 3D structural representation of aquifers (groundwater reservoirs). The innovative contribution of this approach in building 3D hydrostructural subsurface models from a spatial database resides in simplifying the required step-by-step processes by considering a unified compatible combination of “RDBMS-ArcGIS-Arc Hydro Groundwater” technologies. The proposed methodology is illustrated using data from an ongoing project aimed at developing an inventory of the groundwater resources of the Saguenay-Lac-Saint-Jean region, Quebec (Canada).     

A pretest for using logrank or Wilcoxon in the two-sample problem

In a two-sample location-scale model with censored data, the logrank test is asymptotically efficient when the error distribution is extreme minimum value. On the other hand, the Wilcoxon test is asymptotically efficient when the error distribution is logistic. We propose a pretest for choosing between logrank and Wilcoxon by determining if the error distribution is closer to extreme minimum value or logistic. This adaptive test is compared with the logrank and Wilcoxon tests through simulation.