Size-dependent multispectral photoacoustic response of solid and hollow gold nanoparticles

Photoacoustic (PA) imaging attracts a great deal of attention as an innovative modality for longitudinal, non-invasive, functional and molecular imaging in oncology. Gold nanoparticles (AuNPs) are identified as superior, NIR-absorbing PA contrast agents for biomedical applications. Until now, no systematic comparison of the optical extinction and PA efficiency of water-soluble AuNPs of various geometries and small sizes has been performed.Here spherical AuNPs with core diameters of 1.0, 1.4 and 11.2?nm, nanorods with longitudinal/transversal elongation of 38/9 and 44/12?nm and hollow nanospheres with outer/inner diameters of 33/19, 57/30, 68/45 and 85/56?nm were synthesized. The diode laser set-up with excitations at 650, 808, 850 and 905?nm allowed us to correlate the molar PA signal intensity with the molar extinction of the respective AuNPs. Deviations were explained by differences in heat transfer from the particle to the medium and, for larger particles, by the scattering of light. The molar PA intensity of 1.0?nm AuNPs was comparable to the commonly used organic dye methylene blue, and rapidly increased with the lateral size of AuNPs.     

Investigating Reliability on Fuel Cell Model Identification. Part II: An Estimation Method for Stochastic Parameters

An alternative way to process data from polarization measurements for fuel cell model validation is proposed. The method is based on re‐ and subsampling of I–V data, with which repetitive estimations are obtained for the model parameters. This way statistics such as standard deviations and correlations between the parameters may be experimentally derived. Histograms may also be produced, approximating the probability distributions that they follow. Two experimental case studies are discussed. In the first case, observations are made on the behavior of the parameter values for two mathematical models. As the number of data points (measurement points) employed in the estimation of the parameters increases, parameters with high variances converge to specific values. On the contrary, parameters with small variances diverge linearly. The parameters' histograms do not usually follow normal distributions rather they show a connection between the number of peaks in the graphs and correlations of the parameters. The second case study is an application on a fast degraded SOFC button cell, where the values and the histograms of the parameters are compared before and after degradation.     

Chemical Expansion Due to Hydration of Proton‐Conducting Perovskite Oxide Ceramics

The crystal structures of proton‐conducting BaZr_1?xY_xO_3?x/2 (BZY05–BZY20) and BaCe_0.8Y_0.2O_2.9 (BCY20) during hydration/dehydration has been studied by in situ high‐temperature X‐ray diffraction and thermal analysis. A contraction/expansion of the crystal lattice associated with dehydration/hydration was observed for all materials at elevated temperatures and the polymorphic phase transition temperatures of BaCe_0.8Y_0.2O_2.9 were depressed by lowering the vapor pressure of water. A thermodynamic formalism is introduced to describe the chemical expansion associated with the hydration of oxygen vacancies in acceptor‐doped oxides. A conventional point defect model was applied to describe the lattice strain associated with the hydration. The chemical expansion is discussed with respect to the available volumetric data on the hydration of proton‐conducting oxide materials and its likely impact on ceramic fuel cells/hydrogen separation membranes utilizing a proton‐conducting electrolyte.     

Small‐Molecule Inhibitors of AF6 PDZ‐Mediated Protein–Protein Interactions

PDZ (PSD‐95, Dlg, ZO‐1) domains are ubiquitous interaction modules that are involved in many cellular signal transduction pathways. Interference with PDZ‐mediated protein–protein interactions has important implications in disease‐related signaling processes. For this reason, PDZ domains have gained attention as potential targets for inhibitor design and, in the long run, drug development. Herein we report the development of small molecules to probe the function of the PDZ domain from human AF6 (ALL1‐fused gene from chromosome 6), which is an essential component of cell–cell junctions. These compounds bind to AF6 PDZ with substantially higher affinity than the peptide (Ile‐Gln‐Ser‐Val‐Glu‐Val) derived from its natural ligand, EphB2. In intact cells, the compounds inhibit the AF6–Bcr interaction and interfere with epidermal growth factor (EGF)‐dependent signaling.     

Critical conditions for onset of mould growth under varying climate conditions

A performance-based service life design format based on climatic exposure on one hand and “resistance” of materials against mould growth on the other hand, is presented in this paper. A limit state for onset of mould growth is defined as the occurrence of traces of mould observed by microscopy. A dose–response model is proposed by which onset of mould growth can be predicted for an arbitrary climate history of combined relative humidity ? and temperature T. The model is calibrated and verified against a comprehensive set of experimental data published by Viitanen et al. [Viitanen H, Ritschkoff A-C. Mould growth in pine and spruce sapwood in relation to air humidity and temperature. Uppsala: Swedish University of Agricultural Sciences, Department of Forest Products; 1991. Report No. 221, 49 p.; Viitanen H. Modelling the time factor in the development of mould fungi – effect of critical humidity and temperature conditions in pine and spruce sapwood. Holzforschung 1997;51(1):6–14; Viitanen H. Modelling the time factor in the development of brown rot decay in pine and spruce sapwood – the effect of critical humidity and temperature conditions. Holzforschung 1997;51(2):99–106; Viitanen H, Bjurman J. Mould growth on wood under fluctuating humidity conditions. Material und Organismen 1995;29(1):27–46] describing mould development on spruce and pine sapwood as a function of climatic exposure. The model is applied to predict time to onset of mould growth under natural outdoor climate (under shelter) as well as mould development in building attics and in crawl space foundations. The predicted response shows reasonable agreement with experimental observations and proven experience, although biological processes of this type display great variability. The results show that a generally applicable, quantitative model can be used as a powerful tool for moisture safe design in practice. The model is designed to facilitate continuous improvement of prediction capability by further laboratory testing of various materials under specified climate conditions. In combination with currently available building physics software the model is suitable for moisture safe design of wood-based components in the building envelope.     

Mobilization of lead and other trace elements following shock chlorination of wells

Many owners of domestic wells shock chlorinate their wells to treat for bacterial contamination or control bad odors from sulfides. Analysis of well water with four wells from Fallon, Nevada, showed that following recommended procedures for shock chlorinating wells can cause large, short-lasting increases in trace-element concentrations in ground water, particularly for Cu, Fe, Pb, and Zn. Lead concentrations increased up to 745 fold between samples collected just before the well was shock chlorinated and the first sample collected 22-24 h later; Zn concentrations increased up to 252 fold, Fe concentrations increased up to 114 fold, and Cu concentrations increased up to 29 fold. Lead concentrations returned to near background levels following pumping of about one casing volume, however, in one well an estimated 120 mg of excess Pb were pumped before concentrations returned to prechlorination levels. Total Pb concentrations were much greater than filtered (0.45 microm) concentrations, indicating the excess Pb is principally particulate. Recommended procedures for purging treated wells following shock chlorination may be ineffective because a strong NaOCl solution can remain in the casing above the pump even following extended pumping. Only small changes in gross alpha and beta radioactivity occurred following shock chlorination. USEPA has not promulgated drinking-water standards for 210Pb, however, measured 210Pb activities in the study area typically were less than the Canadian Maximum Acceptable Concentration of 100 mBq/L. By consuming well water shortly after shock chlorination the public may inadvertently be exposed to levels of Pb, and possibly 210Pb, that exceed drinking-water standards.     

Inter-generational ties, financial transfers and home-ownership support

Home-ownership is transmitted between generations. Parental gifts form one of the mechanisms through which the intergenerational transmission of home-ownership takes place. Using the Netherlands Kinship Panel Study, we investigated the influence of parental and children’s resources and other characteristics on financial support from parents to children. A major independent variable was parental home-ownership. As dependent variables, we distinguished between financial support towards buying a home, and financial support in the form of gifts of € 5,000 or more ever received. By making this distinction, we could test whether homeowner parents were particularly likely to help their children become homeowners rather than giving other types of financial help. The results did not indicate such specific gift-giving: parental home-ownership was just as important to other types of monetary support as to home-ownership support. However, the distance to the place where the adult child had grown up was negatively associated with receiving home-ownership support but not with receiving other financial transfers.     

The Formation of Calcified Nanospherites during Micropetrosis Represents a Unique Mineralization Mechanism in Aged Human Bone

Osteocytes—the central regulators of bone remodeling—are enclosed in a network of microcavities (lacunae) and nanocanals (canaliculi) pervading the mineralized bone. In a hitherto obscure process related to aging and disease, local plugs in the lacuno‐canalicular network disrupt cellular communication and impede bone homeostasis. By utilizing a suite of high‐resolution imaging and physics‐based techniques, it is shown here that the local plugs develop by accumulation and fusion of calcified nanospherites in lacunae and canaliculi (micropetrosis). Two distinctive nanospherites phenotypes are found to originate from different osteocytic elements. A substantial deviation in the spherites' composition in comparison to mineralized bone further suggests a mineralization process unlike regular bone mineralization. Clearly, mineralization of osteocyte lacunae qualifies as a strong marker for degrading bone material quality in skeletal aging. The understanding of micropetrosis may guide future therapeutics toward preserving osteocyte viability to maintain mechanical competence and fracture resistance of bone in elderly individuals.