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排序方式: 共有1677条查询结果,搜索用时 11 毫秒
21.
Flavien PilletAuthor Vitae Carmen RomeraAuthor Vitae Emmanuelle TrévisiolAuthor Vitae Sophie BellonAuthor VitaeMarie-Paule Teulade-FichouAuthor Vitae Jean-Marie FrançoisAuthor Vitae Geneviève PratvielAuthor Vitae Véronique Anton LeberreAuthor Vitae 《Sensors and actuators. B, Chemical》2011,157(1):304-309
Surface plasmon resonance imaging (SPRi) is a label free technology for biomolecular interaction, which gives access to binding kinetic parameters from real time acquisition. It offers the possibility to test in a single run a large number of interactions, allowing rapid identification of the most suitable compounds toward a given biological entity. Until now, this technique has proven to be relevant for interaction between relatively large molecules (protein, antibodies, DNA) but has not been challenged yet for the screening of small molecules that can be of interested in the field of drug discovery. As a proof a principle, we have used SPRi to screen for interaction of several small molecules, referred to as G4-ligands on G-quadruplex DNA. This technology allowed to easy discrimination of the binding properties of four G4-ligands on quadruplex DNA models. 相似文献
22.
Daniela PatrascuAuthor VitaeIulia DavidAuthor Vitae Vasile DavidAuthor VitaeConstantin MihailciucAuthor Vitae Ioan StamatinAuthor VitaeJean CiureaAuthor Vitae Livia NagyAuthor VitaeGéza NagyAuthor Vitae Anton Alexandru CiucuAuthor Vitae 《Sensors and actuators. B, Chemical》2011,156(2):731-736
Iron(II) phthalocyanine (FePc) modified multi-wall carbon nanotubes paste electrodes (MWCNTPEs) were used as voltammetric sensors to selectively detect dopamine (DA) in the presence of serotonin (5-HT). The electrochemical behavior of DA at the new modified electrode was investigated using CV. The enhanced current response of DA indicates that FePc modification of the MWCNTPE surface results in a high catalytic activity for the redox reaction of DA. Differential pulse voltammetry was applied in detection of DA and 5-HT at FePc-MWCNTPE. The method parameters were optimized. Detection limit of 2.05 × 10−7 M was obtained for DA by using the electrocatalytic oxidation signal corresponding to the FeII/FeIII redox process. The separation between the peak potentials of DA and 5-HT is 170 mV which is large enough for the simultaneously, selective determination of the two chemical species in their mixtures. There was no electrochemical response for ascorbic acid (AA) added in the sample. The monoamine neurotransmitter measuring method has been tested in analyzing deproteinized serum samples. 相似文献
23.
James Gray Yang-Hui He Anton Ilderton André Lukas 《Computer Physics Communications》2009,180(1):107-119
We give a simple tutorial introduction to the Mathematica package STRINGVACUA, which is designed to find vacua of string-derived or inspired four-dimensional N=1 supergravities. The package uses powerful algebro-geometric methods, as implemented in the free computer algebra system Singular, but requires no knowledge of the mathematics upon which it is based. A series of easy-to-use Mathematica modules are provided which can be used both in string theory and in more general applications requiring fast polynomial computations. The use of these modules is illustrated throughout with simple examples.
Program summary
Program title: STRINGVACUACatalogue identifier: AEBZ_v1_0Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEBZ_v1_0.htmlProgram obtainable from: CPC Program Library, Queen's University, Belfast, N. IrelandLicensing provisions: GNU GPLNo. of lines in distributed program, including test data, etc.: 31 050No. of bytes in distributed program, including test data, etc.: 163 832Distribution format: tar.gzProgramming language: “Mathematica” syntaxComputer: Home and office spec desktop and laptop machines, networked or stand aloneOperating system: Windows XP (with Cygwin), Linux, Mac OS, running Mathematica V5 or aboveRAM: Varies greatly depending on calculation to be performedClassification: 11.1External routines: Linux: The program “Singular” is called from Mathematica. Windows: “Singular” is called within the Cygwin environment from Mathematica.Nature of problem: A central problem of string-phenomenology is to find stable vacua in the four-dimensional effective theories which result from compactification.Solution method: We present an algorithmic method, which uses techniques of algebraic geometry, to find all of the vacua of any given string-phenomenological system in a huge class.Running time: Varies greatly depending on calculation requested. 相似文献24.
Oriol Falivene Patricia Cabello Pau Arbués Josep Anton Muñoz Lluís Cabrera 《Computers & Geosciences》2009,35(8):1642-1651
Valid representations of geological heterogeneity are fundamental inputs for quantitative models used in managing subsurface activities. Consequently, the simulation of realistic facies distributions is a significant aim. Realistic facies distributions are typically obtained by pixel-based, object-based or process-based methods. This work presents a pixel-based geostatistical algorithm suitable for reproducing lateral gradual facies transitions (LGFT) between two adjacent sedimentary bodies. Lateral contact (i.e. interfingering) between distinct depositional facies is a widespread geometric relationship that occurs at different scales in any depositional system. The algorithm is based on the truncation of the sum of a linear expectation trend and a random Gaussian field, and can be conditioned to well data. The implementation introduced herein also includes subroutines to clean and geometrically characterize the obtained LGFT. The cleaned sedimentary body transition provides a more appropriate and realistic facies distribution for some depositional settings. The geometric measures of the LGFT yield an intuitive measure of the morphology of the sedimentary body boundary, which can be compared to analogue data. An example of a LGFT obtained by the algorithm presented herein is also flow simulated, quantitatively demonstrating the importance of realistically reproducing them in subsurface models, if further flow-related accurate predictions are to be made. 相似文献
25.
Sergei V. Gusev Anton S. Shiriaev Leonid B. Freidovich 《International journal of control》2016,89(7):1396-1405
Numerically finding stabilising feedback control laws for linear systems of periodic differential equations is a nontrivial task with no known reliable solutions. The most successful method requires solving matrix differential Riccati equations with periodic coefficients. All previously proposed techniques for solving such equations involve numerical integration of unstable differential equations and consequently fail whenever the period is too large or the coefficients vary too much. Here, a new method for numerical computation of stabilising solutions for matrix differential Riccati equations with periodic coefficients is proposed. Our approach does not involve numerical solution of any differential equations. The approximation for a stabilising solution is found in the form of a trigonometric polynomial, matrix coefficients of which are found solving a specially constructed finite-dimensional semidefinite programming (SDP) problem. This problem is obtained using maximality property of the stabilising solution of the Riccati equation for the associated Riccati inequality and sampling technique. Our previously published numerical comparisons with other methods shows that for a class of problems only this technique provides a working solution. Asymptotic convergence of the computed approximations to the stabilising solution is proved below under the assumption that certain combinations of the key parameters are sufficiently large. Although the rate of convergence is not analysed, it appeared to be exponential in our numerical studies. 相似文献
26.
This work introduces a new algorithm for surface reconstruction in ℝ3 from spatially arranged one-dimensional cross sections embedded in ℝ3. This is generally the case with acoustic signals that pierce an object non-destructively. Continuous deformations (homotopies)
that smoothly reconstruct information between any pair of successive cross sections are derived. The zero level set of the
resulting homotopy field generates the desired surface. Four types of homotopies are suggested that are well suited to generate
a smooth surface. We also provide derivation of necessary higher order homotopies that can generate a C
2 surface. An algorithm to generate surface from acoustic sonar signals is presented with results. Reconstruction accuracies
of the homotopies are compared by means of simulations performed on basic geometric primitives. 相似文献
27.
This paper studies the so‐called inverse filtering and deconvolution problem from different angles. To start with, both exact and almost deconvolution problems are formulated, and the necessary and sufficient conditions for their solvability are investigated. Exact and almost deconvolution problems seek filters that can estimate the unknown inputs of the given plant or system either exactly or almostly whatever may be the unintended or disturbance inputs such as measurement noise, external disturbances, and model uncertainties that act on the system. As such they require strong solvability conditions. To alleviate this, several optimal and suboptimal deconvolution problems are formulated and studied. These problems seek filters that can estimate the unknown inputs of the given system either exactly, almostly or optimally in the absence of unintended (disturbance) inputs, and on the other hand, in the presence of unintended (disturbance) inputs, they seek that the influence of such disturbances on the estimation error be as small as possible in a certain norm (H2 or H∞) sense. Both continuous‐ and discrete‐time systems are considered. For discrete‐time systems, the counter parts of all the above problems when an ??‐step delay in estimation is present are introduced and studied. Next, we focus on the exact and almost deconvolution but this time when the uncertainties in plant dynamics can be structurally modeled by a Δ‐block as a feedback element to the nominally known plant dynamics. This is done either in the presence or absence of external disturbances. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献
28.
Eremeev AV 《Evolutionary computation》2008,16(1):127-147
We consider the optimization problem of finding the best possible offspring as a result of a recombination operator in an evolutionary algorithm, given two parent solutions. The optimal recombination is studied in the case where a vector of binary variables is used as a solution encoding. By means of efficient reductions of the optimal recombination problems (ORPs) we show the polynomial solvability of the ORPs for the maximum weight set packing problem, the minimum weight set partition problem, and for linear Boolean programming problems with at most two variables per inequality, and some other problems. We also identify several NP-hard cases of optimal recombination: the Boolean linear programming problems with three variables per inequality, the knapsack, the set covering, the p-median, and some other problems. 相似文献
29.
In this paper we survey our research on smart meeting rooms and its relevance for augmented reality meeting support and virtual
reality generation of meetings in real time or off-line. The research reported here forms part of the European 5th and 6th
framework programme projects multi-modal meeting manager (M4) and augmented multi-party interaction (AMI). Both projects aim
at building a smart meeting environment that is able to collect multimodal captures of the activities and discussions in a
meeting room, with the aim to use this information as input to tools that allow real-time support, browsing, retrieval and
summarization of meetings. Our aim is to research (semantic) representations of what takes place during meetings in order
to allow generation, e.g. in virtual reality, of meeting activities (discussions, presentations, voting, etc.). Being able
to do so also allows us to look at tools that provide support during a meeting and at tools that allow those not able to be
physically present during a meeting to take part in a virtual way. This may lead to situations where the differences between
real meeting participants, human-controlled virtual participants and (semi-) autonomous virtual participants disappear.
相似文献
Dirk HeylenEmail: |
30.
A rapid method of oxymyoglobin purification 总被引:1,自引:0,他引:1
Oxymyoglobin was isolated from bovine Longissimus lumborum muscle by precipitation with ammonium sulfate and purification in rapid conditions with only one chromatographic step on Mono-Q HR column with a HPLC system. Purity of oxymyoglobin was controlled by SDS-polyacrylamide gel electrophoresis and isoelectrofocusing. 相似文献