Articular cartilage volume in the knee: semiautomated determination from three-dimensional reformations of MR images

PURPOSE: To determine the accuracy of semiautomated quantification of articular cartilage volume from three-dimensional (3D) reformations of magnetic resonance (MR) images. MATERIALS AND METHODS: Sagittal, fat-suppressed, 3D, spoiled gradient-recalled-echo MR imaging of two bovine and two human cadaver knees was performed. Articular cartilage volume was calculated from 3D reformations of the MR images by using a semiautomated program written at the authors' institution. Calculated volumes were compared with directly measured volumes of the surgically removed articular cartilage. RESULTS: The percentage of error of the MR imaging-determined volumes was 6.53% +/- 4.75 (mean +/- standard deviation). A strong correlation between the two sets of observations was shown (r=.997). Linear regression showed the calculated volumes to be highly accurate (slope=1.002, P>.25). Repeated reformations yielded volumes that were reproducible (mean absolute error, 0.013 mL +/- 0.019) and not significantly different from the measured volume (P>.10). CONCLUSION: Semiautomated quantification of knee articular cartilage from MR images yields highly accurate cartilage volumes.     

A multisource energy harvesting utilizing highly efficient ferroelectric PMN-PT single crystal

This paper demonstrates a multi-source energy harvester that is able to utilize simultaneously both piezoelectric and pyroelectric effects in lead magnesium niobate-lead titanate (PMN-PT) single crystal. The paper presents a study of PMN-PT single crystal with a (67:33) composition grown in our laboratory via a vertical gradient freeze method without any flux. The performance of the piezoelectric and pyroelectric energy harvester using unimorph device structure was evaluated via modeling and experiment. The theoretical study was implemented based on a distributed parameter electromechanical model and the modelling procedure was approximated using finite element analysis to predict the electromechanical behavior of the harvester. The maximum power density at a resonance frequency of 50 Hz and optimum resistance of 2 MΩ was 16.7 nW/(g² cm³) under a 1 g acceleration of vibration. The measured values of electrical output parameters were in good agreement with theoretical and modelling results using MATLAB and COMSOL Multiphysics, respectively. By using the pyroelectric effect along with the piezoelectric effect, the output voltage of the energy harvester was found to be enhanced at the optimum resistance and specific frequency values. It was noticed that the output voltage was increased monotonically with temperature-difference (ΔT) and reaches up to 180 % of its original value under temperature difference of 1.7 °C at a frequency value of 49 Hz.     

Location of nucleolar organizing regions on the chromosomes of the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus)

Terminal chromosome associations ("satellite associations") in ASG-banded preparations have been used to determine the number and location of staellites in the Syrian hamster (Mesocricetus auratus) and the Djungarian hamster (Phodopus sungorus). Five pairs of satellites are found in the former and four in the latter. Nucleolar organizing regions (NORs) were visualized with the Ag-AS silver precipitation technique, and their number and position corresponded exactly with the number and position of satellites in Phodopus, where positive chromosome identification can be made in the absence of banding. Numerical agreement is exact in Mesocricetus as well, and the morphology of the silver-tagged NOR-bearing chromosomes strongly suggests that corrrespondence also occurs in this species.     

Natural frequencies of functionally graded plates by a meshless method

We use the global collocation method, the first and the third-order shear deformation plate theories, the Mori–Tanaka technique to homogenize material properties, and approximate the trial solution with multiquadric radial basis functions to analyze free vibrations of functionally graded plates. Frequencies computed by the present method are found to agree well with those from the analytical solution of Vel and Batra, and the numerical solution of Qian et al. based on the meshless local Petrov–Galerkin formulation.     

Multi-objective optimization of the ecm process

Optimization of process parameters is helpful in efficient working of the process and, hence, in lowering the cost of machining. Optimization of ECM process parameters has been achieved by considering only one objective at a time from metal removal rate, geometrical accuracy, and total process cost. From a practical point of view, a solution of the ecm problem satisfying all three objectives simultaneously is highly desirable.In the proposed model, a multi-objective problem involving the ecm process is formulated producing highly nonlinearized equations. These are then linearized by regression analysis and converted into a goal programming format. Finally, the problem is solved by the partitioning algorithm.It is concluded that the tool, or cathode, remains safe at the optimal values of design variables obtained in the examples discussed. The optimal value of voltage when metal removal rate is the only objective, is found to be higher than the case when the geometrical accuracy requirement is also to be satisfied.     

Cyclic stretch down-regulates calcium transporter gene expression in neonatal rat ventricular myocytes

Abnormal intracellular Ca2+ handling in hypertrophied and failing hearts is partly due to changes in Ca2+ transporter gene expression, but the mechanisms responsible for these alterations remain largely unknown. We previously showed that intrinsic mechanical load (i.e. spontaneous contractile activity) induced myocyte hypertrophy, and down-regulated SR Ca2+ ATPase (SERCA2) gene expression in cultured neonatal rat ventricular myocytes (NRVM). In the present study, we examined whether extrinsic mechanical load (i.e. cyclic stretch) also induced NRVM hypertrophy, and led to down-regulation of SERCA2 and other Ca2+ transporter genes which have been associated with cardiac hypertrophy and failure in vivo. NRVM were maintained in serum-free culture medium under control conditions, or subjected to cyclic mechanical deformation (1.0 Hz, 20% maximal strain, 48 h). Under these conditions, cyclic stretch induced NRVM hypertrophy, as evidenced by significant increases in total protein/DNA ratio, myosin heavy chain (MHC) content, and atrial natriuretic factor (ANF) secretion. Cyclic stretch also induced the MHC isoenzyme "switch" which is characteristic of hemodynamic overload of the rat heart in vivo. Cyclic stretch significantly down-regulated SERCA2 and ryanodine receptor (RyR) mRNA and protein levels, while simultaneously increasing ANF mRNA. In contrast, Na+-Ca2+ exchanger and phospholamban mRNA levels were unaffected. Load-dependent SERCA2 and RyR down-regulation was independent of Ca2+ influx via voltage-gated, L-type Ca2+ channels, as cyclic stretch down-regulated SERCA2 and RyR mRNA levels in both control and verapamil-treated NRVM. These results indicate that extrinsic mechanical load (in the absence of other exogenous stimuli) induces NRVM hypertrophy and causes down-regulation of Ca2+ transporter gene expression. This in vitro model system should prove useful to dissect the intracellular signaling pathways responsible for transducing this phenotype during cardiac hypertrophy and heart failure in vivo.     

Quasistatic indentation of a rubber-covered roll by a rigid roll

The nonlinear elastic problem involving the indentation of a slightly compressible rubber-like layer bonded to a rigid cylinder and indented by another rigid cylinder is analysed by the finite element method. Both the geometric and material nonlinearities are accounted for. The finite element formulation of the problem is based upon a variational principle recently proposed by Cescotto and Fonder, and is valid for both slightly compressible and incompressible materials. The results computed and presented graphically include the shape of the indented surface, the pressure distribution over the contact surface, and the stress distribution at the bond surface. For the same contact width, the results for the compressible material are found to differ significantly from those for the case when the rubber-like layer is assumed to be incompressible.     

Factors affecting length of herdlife in purebred and crossbred dairy cattle

The proportional hazards model with censoring was used to assess the effects of breeding value, disease, calving, size, and udder and lactation traits on length of herdlife of 3881 heifers in five herds. Data were recorded over 10 yr from three lines: a Holstein line, an Ayrshire-based line, and a crossbred line. Influences on survival were assessed from data collected at birth, 34, 50, and 82 wk, first freshening, and at 112 and 308 d postpartum. Median estimated herdlife (age at 50% culling) was 3.9 yr for animals alive at first freshening and increased to 4.3 yr for those that completed a first lactation (308 d postpartum). Herds differed greatly in the pattern of culling after freshening. Crossbred females had 21 wk longer median estimated herdlife than the mean of the purelines at 308 d postpartum. Individual milk yield was positively associated with longevity and had the greatest impact on length of herdlife. Abortion and fertility measured as days to last insemination were negatively associated with length of herdlife. Large heifers tended to have increased longevity. High feed intake postpartum was associated with reduced length of herdlife. Objective measures of conformation, which included measurements of the udder, were not important in determining herdlife.     

Design of a Stable DC Voltage Source and Computer Controlling of It Using an Indigenously Developed All-Digital Addressing-Cum-Control Hardware

We have developed a digitally operated addressing and control module (DACM) for addressing and controlling of equipment from a remote computer using a communication protocol developed in-house. This is useful for automation of an experiment that uses multiple equipment in a pre-decided synchronized manner. We also report design of a multipurpose high voltage direct current (DC) source that provides output of 0–100 V with an average stability of 1.90 (36) mV and has minimum step size of 3 mV. Operation of the DACM is examined by selecting the desired equipment, which in this case is the dc source, and remotely controlling its output from a computer. We also show that this can generate voltage with different waveforms within a 0–10 Hz frequency bandwidth. Such computer controlled ultra-stable high voltage sources tuneable to any arbitrary waveforms at low frequencies have many applications such as, driving a piezo for smooth scanning of laser frequencies, tuning length of a Fabry–Perot cavity, biasing of the electrodes in an ion trap and so on.     

Class I alleles of the bovine major histocompatibility system and their association with economic traits

A total of 179 Holstein cows from the Agriculture Canada Research Branch herd at Ottawa and 271 progeny-tested Canadian Holstein bulls were typed for 37 lymphocyte antigens. Each antigen appears to be controlled by a distinct, codominant allele of the bovine lymphocyte antigen (BoLA)-A locus, which is a class I locus of the bovine major histocompatibility system. Only 10 and 16 alleles were present in Holstein cows and bulls, respectively. The association between bovine lymphocyte antigens and economically important traits was examined in gene substitution models. Separate models were used for the cow and bull data. The substitution of the W6.1 allele for the W10 allele was associated with increased protein yield in the milk in both analyses. The results were also consistent with a previous report that the presence of the W10 allele was associated with increased fat percentage compared with some, but not all, of the other alleles. However, more research is necessary to confirm these findings and to determine the biological mechanisms underlying these associations.