全文获取类型
收费全文 | 6052篇 |
免费 | 7篇 |
专业分类
电工技术 | 6篇 |
化学工业 | 276篇 |
机械仪表 | 11篇 |
建筑科学 | 6篇 |
矿业工程 | 1篇 |
能源动力 | 5篇 |
轻工业 | 518篇 |
水利工程 | 1篇 |
石油天然气 | 1篇 |
无线电 | 13篇 |
一般工业技术 | 51篇 |
冶金工业 | 5147篇 |
原子能技术 | 2篇 |
自动化技术 | 21篇 |
出版年
2013年 | 76篇 |
2011年 | 5篇 |
2010年 | 6篇 |
2005年 | 7篇 |
2004年 | 7篇 |
2003年 | 7篇 |
2000年 | 5篇 |
1999年 | 138篇 |
1998年 | 1526篇 |
1997年 | 860篇 |
1996年 | 581篇 |
1995年 | 338篇 |
1994年 | 312篇 |
1993年 | 331篇 |
1992年 | 58篇 |
1991年 | 102篇 |
1990年 | 73篇 |
1989年 | 99篇 |
1988年 | 107篇 |
1987年 | 99篇 |
1986年 | 91篇 |
1985年 | 58篇 |
1984年 | 26篇 |
1983年 | 33篇 |
1982年 | 46篇 |
1981年 | 48篇 |
1980年 | 68篇 |
1979年 | 25篇 |
1978年 | 50篇 |
1977年 | 163篇 |
1976年 | 368篇 |
1975年 | 41篇 |
1974年 | 24篇 |
1973年 | 24篇 |
1972年 | 31篇 |
1971年 | 28篇 |
1970年 | 22篇 |
1969年 | 11篇 |
1968年 | 10篇 |
1967年 | 12篇 |
1966年 | 12篇 |
1965年 | 14篇 |
1964年 | 11篇 |
1963年 | 8篇 |
1959年 | 6篇 |
1958年 | 8篇 |
1957年 | 5篇 |
1956年 | 10篇 |
1955年 | 7篇 |
1947年 | 4篇 |
排序方式: 共有6059条查询结果,搜索用时 9 毫秒
151.
THE DESIGN OF EXPERIMENTS FOR SHELF LIFE STUDY 总被引:13,自引:0,他引:13
152.
Vitamin contents of peas were measured at various stages of raw product handling, during 1976–1979 seasons, on different cultivars, on different sizes of peas, at various stages of processing, and at different processing plants. Some cultivar differences were shown in ascorbic acid, carotene, and folic acid, and different sizes of peas contained significantly different amounts of ascorbic acid, carotene, and thiamin contents. Profound effects were observed during blanching and thermal processing of peas. Ascorbic acid, thiamin, vitamin B6, and niacin contents of canned peas were significantly (95% level) lower than those of fresh peas. Also some significant differences in vitamin contents of canned peas among different processing plants were observed. 相似文献
153.
The complete phase-equilibrium diagram of the system CaF2 -AlF3 -Na3 AlF6 and the subsolidus portion of the system CaF2 -AlF3 -Na3 AlF6 -Al2 O3 were established from microscopic, powder X-ray diffraction, quench, and DTA data obtained from samples encapsulated in sealed tubes and either reacted in the solid state or melted and recrystallized. The system Na3 AlF6 -CaF2 contains a simple eutectic with no compound formation or solid solution. The system CaF2 -AlF3 -Na3 AlF6 contains two ternary compounds, NaCaAlF6 and NaCaAl2 F9 , which melt incongruently at 735° and 712°C, respectively; NaCaAlF6 exists in three polymorphic forms with transitions at 610° and 722°C and NaCaAl2 F9 is body-centered cubic with a0 = 10.765 Å. The three binary and two ternary compounds divide the system into eight compatibility triangles. Along the NaCaAlF6 -Na3 AlF6 join, 7 mol% NaCaAlF6 is soluble in α-cryolite at 525° and 42% in β-cryolite at 731°C. The quaternary system Na3 AlF6 -AlF3 -CaF2 -Al2 O3 contains eight compatibility tetrahedra. 相似文献
154.
ELIZABETH J. FORDYCE RICHARD M. FORBES KELLY R. ROBBINS JOHN W. ERDMAN JR. 《Journal of food science》1987,52(2):440-444
The utility of the phytate/zinc and phytate × calcium/zinc molar ratios for predicting zinc bioavailability from processed soybean foods was investigated. Weight gain and bone zinc accumulation in rats fed various soy protein products were plotted against the calculated molar ratios. The phytate × calcium/zinc ratio was a better predictor of zinc bioavailability in similarly processed products than was the phytate/ zinc ratio. However, in some cases the phytate × calcium/zinc ratio was not effective since some processing procedures apparently altered binding of phytic acid to minerals and other food components. 相似文献
155.
Calcium bioavailability (BV) from sesame seeds, almond powder, whole wheat bread, spinach, and nonfat dry milk (NFDM) was compared to calcium BV from a calcium carbonate (CaCO3)-supplemented control diet using a rat model. When comparing different calcium sources, the relative BV of the products was CaCO3 (100%), NFDM (100%) whole wheat bread (95%), almond powder (66%), sesame seeds (65%), NFDM and spinach mixture (52%), and spinach (47%). Separate almond, NFDM, and CaCO3 diets were supplemented with 0.4% ascorbic acid; vitamin C addition had no significant effect on calcium BV. 相似文献
156.
TE Dever R Sripriya JR McLachlin J Lu JR Fabian SR Kimball LK Miller 《Canadian Metallurgical Quarterly》1998,95(8):4164-4169
Phosphorylation of eukaryotic translation initiation factor 2alpha (eIF2alpha) is a common cellular mechanism to limit protein synthesis in stress conditions. Baculovirus PK2, which resembles the C-terminal half of a protein kinase domain, was found to inhibit both human and yeast eIF2alpha kinases. Insect cells infected with wild-type, but not pk2-deleted, baculovirus exhibited reduced eIF2alpha phosphorylation and increased translational activity. The negative regulatory effect of human protein kinase RNA-regulated (PKR), an eIF2alpha kinase, on virus production was counteracted by PK2, indicating that baculoviruses have evolved a unique strategy for disrupting a host stress response. PK2 was found in complex with PKR and blocked kinase autophosphorylation in vivo, suggesting a mechanism of kinase inhibition mediated by interaction between truncated and intact kinase domains. 相似文献
157.
Higher levels of thymidylate synthase gene expression are observed in pulmonary as compared with hepatic metastases of colorectal adenocarcinoma 总被引:1,自引:0,他引:1
158.
EA Fyrberg CC Fyrberg JR Biggs D Saville CJ Beall A Ketchum 《Canadian Metallurgical Quarterly》1998,36(7-8):271-287
We show that different Drosophila actin isoforms are not interchangeable. We sequenced the six genes that encode conventional Drosophila actins and found that they specify amino acid replacements in 27 of 376 positions. To test the significance of these changes we used directed mutagenesis to introduce 10 such conversions, independently, into the Act88F flight muscle-specific actin gene. We challenged these variant actins to replace the native protein by transforming germline chromosomes of a Drosophila strain lacking flight muscle actin. Only one of the 10 reproducibly perturbed myofibrillar function, demonstrating that most isoform-specific amino acid replacements are of minor significance. In order to establish the consequences of multiple amino acid replacements, we substituted portions of the Drosophila Act88F actin gene with corresponding regions of genes encoding other isoforms. Only one of five constructs tested engendered normally functioning flight muscles, and the severity of myofibrillar defects correlated with the number of replacements within the chimeric genes. Finally, we completely converted the flight muscle actin-encoding gene to one specifying a nonmuscle isoform, a change entailing a total of 18 amino acid replacements. Transformation of flies with this construct resulted in disruption of flight muscle structure and function. We conclude that actin isoform sequences are not equivalent and that effects of the amino acid replacements, while minor individually, collectively confer unique properties. 相似文献
159.
AC Rosa AT Mariano AM Pereira A Tibana TA Gomes JR Andrade 《Canadian Metallurgical Quarterly》1998,47(9):781-790
Faeces from urban children < 2 years old with acute diarrhoeal illness and from non-diarrhoeal infants (controls) were examined for Escherichia coli and other enteropathogens. A total of 990 E. coli isolates from 100 patients and 50 controls was tested for enteropathogenic E. coli (EPEC) serotype (O:H), adherence to HEp-2 cells after incubation for 3 and 6 h, fluorescent actin staining (FAS), DNA hybridisation with EAF, eaeA, STh, STp and EAggEC probes and production of heat-labile enterotoxin (LT) and verocytotoxin (VT) with Y1 and Vero cells. EPEC were the most prevalent enteropathogens in patients (32.7%; and 14% in controls). Enteroinvasive E. coli (EIEC) and Vero cytotoxin-producing E. coli (VTEC) were not detected. The rate of isolation of enterotoxigenic E. coli (ETEC) was identical in both groups. Among the EPEC isolates the prevalent serotypes were O111:H2, O55:NM and O119:H6. Localised adherence (LA) was found significantly more frequently in isolates from patients (19.6%) than controls (2.1%). All LA-positive EPEC isolates were FAS+ and eaeA+, but only 75.2% of them hybridised with the EAF probe. Diffusely adhering E. coli (DAEC) and enteroaggregative E. coli (EAggEC) were found with equal frequency in patients and controls. Twenty-seven E. coli isolates were negative for EAF but positive for eaeA and FAS and produced LA in 6-h adherence tests. These EAF-/eaeA+ strains were the only putative enteropathogen identified in seven patients and were not found in controls. The ability of these strains to elicit ultrastructural cell alterations and cell-signalling events was evaluated in Caco-2 cells (human colon carcinoma cell line) by the gentamicin invasion assay and by transmission electron microscopy. The numbers of intracellular bacteria in cell invasion tests varied from 0.4% to 1.6% of the cell-associated bacteria after a 6-h incubation period. Tyrosine phosphorylation of host cell proteins was assessed in HEp-2 cells by immunofluorescence microscopy and all strains gave positive results. EAF-/eaeA+ E. coli strains express most of the virulence properties found among true EPEC strains and can be a relevant cause of infant diarrhoea in developing countries. 相似文献
160.
EH Herman J Zhang BB Hasinoff KT Tran DP Chadwick JR Clark VJ Ferrans 《Canadian Metallurgical Quarterly》1998,128(1):35-52
We have determined the time course, the spatial spread in brain tissue, and the intracellular distribution of biotin- and fluorescein-labeled phosphorothioate oligodeoxynucleotides (ODNs) following single injections into the rat striatum or the lateral ventricle. These time and space parameters were correlated with the ability of c-fos phosphorothioate antisense ODNs to suppress the induction of Fos protein by cocaine. A rapid and dose-dependent tissue penetration of labeled ODNs was observed following either intrastriatal or intraventricular injections of a constant sample volume. Inspection of tissue sections by confocal microscopy uncovered a distinct change in the intracellular disposition of labeled ODNs during the 24 h post-injection period. At 1, 6 and 12 h, the vast majority of the fluorescent signal was confined to the interstitial spaces throughout the zone penetrated by ODNs. Neuronal nuclei displayed faint labeling along the outer portion of the nucleus at 1 and 6 h post-injection. At these time-points, ODNs were not detected in the cytoplasm. By 16 h, ODNs were barely detectable in the extracellular space and absent from neuronal nuclei. Instead, ODNs were seen in large cytoplasmic granules of neurons throughout the tissue zone penetrated by the ODNs. Experiments with intrastriatal injections of antisense ODNs to c-fos mRNA revealed Fos suppression between 3 and 12 h, but not at 16 and 24 h. This combined analysis has revealed that (1) restricted tissue penetration by ODNs limits their antisense effects on protein expression, and (2) depletion of extracellular ODNs and sequestration of c-fos antisense ODNs into large intracellular granules coincides with the loss of their biological activity. 相似文献