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51.
Clarke DT Botchway SW Coles BC Needham SR Roberts SK Rolfe DJ Tynan CJ Ward AD Webb SE Yadav R Zanetti-Domingues L Martin-Fernandez ML 《The Review of scientific instruments》2011,82(9):093705
Optics clustered to output unique solutions (OCTOPUS) is a microscopy platform that combines single molecule and ensemble imaging methodologies. A novel aspect of OCTOPUS is its laser excitation system, which consists of a central core of interlocked continuous wave and pulsed laser sources, launched into optical fibres and linked via laser combiners. Fibres are plugged into wall-mounted patch panels that reach microscopy end-stations in adjacent rooms. This allows multiple tailor-made combinations of laser colours and time characteristics to be shared by different end-stations minimising the need for laser duplications. This setup brings significant benefits in terms of cost effectiveness, ease of operation, and user safety. The modular nature of OCTOPUS also facilitates the addition of new techniques as required, allowing the use of existing lasers in new microscopes while retaining the ability to run the established parts of the facility. To date, techniques interlinked are multi-photon/multicolour confocal fluorescence lifetime imaging for several modalities of fluorescence resonance energy transfer (FRET) and time-resolved anisotropy, total internal reflection fluorescence, single molecule imaging of single pair FRET, single molecule fluorescence polarisation, particle tracking, and optical tweezers. Here, we use a well-studied system, the epidermal growth factor receptor network, to illustrate how OCTOPUS can aid in the investigation of complex biological phenomena. 相似文献
52.
Peter J. Patsula Benjamin H. Detenber Yin-Leng Theng 《International journal of human-computer interaction》2013,29(7):675-702
Grounded in cognitive load theory, levels of processing theory, modes of learning research, and the facets of working memory (WM) framework, the primary goal of this investigation was to seek empirical evidence for a structure processing mechanism in WM that facilitates the coordination of associations made into coherent structures and the role this mechanism might play in performance and retention. This goal was motivated by a gap in the understanding of mental model production and encoding processes in WM. To carry out this goal, two usability studies were conducted to investigate retention, error, and time performance of Web-based menus created with structured or unstructured design. Relationships between these measures and coordination ability in WM, as well as storage and supervision ability, were also assessed. In Study 1, which tested simple Web-based menus, correlations of .28, ?.56, and ?.54 were detected between coordination (CC) and retention scores, CC and errors, and CC and time; in Study 2, which tested commercial Web-based menus, correlations of .46, ?.45, and ?.25 were detected. For Studies 1 and 2, differences in favor of structured design for retention, error, and time performance were highly significant (p < .001). 相似文献
53.
We present an odometry‐free three‐dimensional (3D) point cloud registration strategy for outdoor environments based on area attributed planar patches. The approach is split into three steps. The first step is to segment each point cloud into planar segments, utilizing a cached‐octree region growing algorithm, which does not require the 2.5D image‐like structure of organized point clouds. The second step is to calculate the area of each segment based on small local faces inspired by the idea of surface integrals. The third step is to find segment correspondences between overlapping point clouds using a search algorithm, and compute the transformation from determined correspondences. The transformation is searched globally so as to maximize a spherical correlation‐like metric by enumerating solutions derived from potential segment correspondences. The novelty of this step is that only the area and plane parameters of each segment are employed, and no prior pose estimation from other sensors is required. Four datasets have been used to evaluate the proposed approach, three of which are publicly available and one that stems from our custom‐built platform. Based on these datasets, the following evaluations have been done: segmentation speed benchmarking, segment area calculation accuracy and speed benchmarking, processing data acquired by scanners with different fields of view, comparison with the iterative closest point algorithm, robustness with respect to occlusions and partial observations, and registration accuracy compared to ground truth. Experimental results confirm that the approach offers an alternative to state‐of‐the‐art algorithms in plane‐rich environments. 相似文献
54.
Michael Wand Alexander Berner Martin Bokeloh Philipp Jenke Arno Fleck Mark Hoffmann Benjamin Maier Dirk Staneker Andreas Schilling Hans-Peter Seidel 《Computers & Graphics》2008,32(2):204-220
This paper describes a new out-of-core multi-resolution data structure for real-time visualization, interactive editing and externally efficient processing of large point clouds. We describe an editing system that makes use of the novel data structure to provide interactive editing and preprocessing tools for large scanner data sets. Using the new data structure, we provide a complete tool chain for 3D scanner data processing, from data preprocessing and filtering to manual touch-up and real-time visualization. In particular, we describe an out-of-core outlier removal and bilateral geometry filtering algorithm, a toolset for interactive selection, painting, transformation, and filtering of huge out-of-core point-cloud data sets and a real-time rendering algorithm, which all use the same data structure as storage backend. The interactive tools work in real-time for small model modifications. For large scale editing operations, we employ a two-resolution approach where editing is planned in real-time and executed in an externally efficient offline computation afterwards. We evaluate our implementation on example data sets of sizes up to 63 GB, demonstrating that the proposed technique can be used effectively in real-world applications. 相似文献
55.
Thomas F. O'Dwyer Benjamin K. Hodnett 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》1995,62(1):30-37
Adsorption isotherms for sodium and trivalent chromium uptake from aqueous solutions onto Amberlite resin were prepared at 18°C. Adsorption of each cation followed the Langmuir model. The rate of uptake of each cation was found to be film diffusion controlled with sodium showing the most rapid uptake. In aqueous solutions containing both chromium and sodium as the only cationic species, it was found that with increasing initial concentration of sodium, the trivalent chromium uptake on the resin decreased substantially. To overcome this difficulty a four step redox–adsorption system has been developed for the removal of Cr3+ from tannery effluents. The first step comprises the oxidation of trivalent chromium to the hexavalent form using selected common oxidising agents. The liquid effluent is then passed through an Amberlite cation-exchange resin in step 2 where the sodium in the waste stream is completely removed. The anionic hexavalent form of chromium (Cr2O) passes unaltered through the resin along with the waste stream. In the third stage the dichromate is reduced back to the trivalent cationic form which is subsequently removed from the waste stream by a second Amberlite ion-exchange bed in stage 4. Each step in this process is assessed in batch and flow mode using simulated and real tannery effluents. 相似文献
56.
Lin Pei-Chun Yankson Benjamin Chauhan Vishal Tsukada Manabu 《The Journal of supercomputing》2022,78(13):15060-15088
The Journal of Supercomputing - Currently, many smart speakers, even social robots, appear on the market to help people's lives become more convenient. Usually, people use smart speakers to... 相似文献
57.
Optimized Photoinitiator for Fast Two‐Photon Absorption Polymerization of Polyester‐Macromers for Tissue Engineering 下载免费PDF全文
58.
Manipulating Electrical and Fluidic Access in Integrated Nanopore‐Microfluidic Arrays Using Microvalves 下载免费PDF全文
59.
High Figure of Merit (FOM) of Bragg Modes in Au‐Coated Nanodisk Arrays for Plasmonic Sensing 下载免费PDF全文
Maxime Couture Thibault Brulé Stacey Laing Wenli Cui Mitradeep Sarkar Benjamin Charron Karen Faulds Wei Peng Michael Canva Jean‐Francois Masson 《Small (Weinheim an der Bergstrasse, Germany)》2017,13(38)
Gold‐coated nanodisk arrays of nearly micron periodicity are reported that have high figure of merit (FOM) and sensitivity necessary for plasmonic refractometric sensing, with the added benefit of suitability for surface‐enhanced Raman scattering (SERS), large‐scale microfabrication using standard photolithographic techniques and a simple instrumental setup. Gold nanodisk arrays are covered with a gold layer to excite the Bragg modes (BM), which are the propagative surface plasmons localized by the diffraction from the disk array. This generates surface‐guided modes, localized as standing waves, leading to highly confined fields confirmed by a mapping of the SERS intensity and numerical simulations with 3D finite element method. The optimal gold‐coated nanodisk arrays are applied for refractometric sensing in transmission spectroscopy with better performance than nanohole arrays and they are integrated to a 96‐well plate reader for detection of IgY proteins in the nanometer range in PBS. The potential for sensing in biofluids is assessed with IgG detection in 1:1 diluted urine. The structure exhibits a high FOM of up to 46, exceeding the FOM of structures supporting surface plasmon polaritons and comparable to more complex nanostructures, demonstrating that subwavelength features are not necessary for high‐performance plasmonic sensing. 相似文献
60.