Purification and Characterization of β-amylase from Bacillus polymyxa No. 26-1

An extracellular β-amylase, which was easily adsorbable onto raw corn starch, was purified 22.5-fold from a new isolate of Bacillus polymyxa No 26–1 with a M_r of 53 kDa and pI of 9.1. The optimum temperature was 45°C and pH 5.5 for raw corn starch. Thermal stability at 40°C and pH stability at 5.0–8.5 were shown. The degradation ofraw starch by β-amylase was greatly stimulated by pullulanase addition. Scanning electron micrographs revealed that starch granule degradation by the enzyme alone occured at the equatorial grooves of lecticular granules. Corn starch granules hydrolyzed by β-amylase had large holes on granule surfaces.     

Dress and Ideology,Fashioning Identity from Antiquity to the Present

Abstract

John Redfern's name appears frequently in the history of couture and late nineteenth- and early twentieth-century fashion, but information on his business is limited. The following is based on research done for the author's MA in the history of dress at the Courtauld Institute in 1993. It examines John Redfern's early years as a draper and how by 1892, he had become the leading ladies' tailor in Britain and France.     

地震激励邻接高层建筑的随机最优控制

采用控制设备联接相邻的高层建筑以降低其地震响应是一个切实有效的方法。基于随机动态规划原理与随机平均法,提出耦合相邻高层建筑的随机最优控制方法。先建立任意层数并在任意层高处控制联接的耦合结构的缩聚模型,再运用随机平均法导出关于模态能量的oIt随机微分方程,应用随机动态规划原理建立动态规划方程,由此可确定最优控制律。将结构的响应控制化为模态能量控制,缩减控制系统的维数。用高斯随机过程模拟地震激励,可计及其功率谱特性。数值结果表明该耦合结构随机最优控制方法的有效性。