Cell surface expression of paraneoplastic encephalomyelitis/sensory neuronopathy-associated Hu antigens in small-cell lung cancers and neuroblastomas

BACKGROUND: Serum from patients with small-cell lung cancer-associated paraneoplastic encephalomyelitis/sensory neuronopathy contains autoantibodies recognizing 35- to 40-kDa nuclear antigens present in neurons, small-cell lung cancers, and some neuroblastomas (anti-Hu). AIM: Because the mechanisms by which Hu autoantibodies may contribute to the paraneoplastic syndrome are largely unknown, we sought to examine if Hu antigens are expressed at the plasma membrane of cultured cells from Hu-expressing tumors. METHODS AND RESULTS: Hu-related molecules of 35 to 41 kDa were detected in the membrane of small-cell lung cancers and neuroblastomas using: (1) immunofluorescence, (2) absorption assays, (3) Western blotting on membrane fractions, and (4) surface biotinylation. The antibodies recognizing these membrane components were specifically absorbed by recombinant HuD protein. There was a perfect correlation between nuclear and membrane Hu expression. To determine the purity of the subcellular fractions, their reactivity with antibodies recognizing the A2 nuclear ribonucleoprotein and the cytoplasmic mitogen-activated protein kinase was examined. None of them was detected in the membrane fractions reactive with sera containing Hu antibodies. CONCLUSIONS: Hu-related antigens can be detected both in the nucleus and the membrane of small-cell lung cancer and neuroblastomas. IMPLICATIONS: These results provide an experimental basis for surface binding-mediated pathogenic mechanisms in paraneoplastic encephalomyelitis/sensory neuronopathy and in Hu-expressing tumors.     

原位合成TiB增强钛基复合材料的微观组织研究

在热力学计算纯钛粉与TiB2颗粒生成TiB条件的基础上，采用原位反应粉末冶金技术制备了TiB／Ti复合材料。试验结果表明，采用计算的反应条件可以实现纯钛粉与TiB2颗粒完全反应，反应生成物TiB呈晶须状，TiB晶须在基体中均匀分布，并与基体之间界面平整、干净。     

Shift of the emission lines of aluminum in a laser plasma generated on the surface of a solid target in the atmosphere

Experiments designed to elucidate how the emission lines of the elements of a laser plasma are affected by the presence of the atmosphere are described. It is shown that the generation of a shock wave when the plasma flare is ignited on the surface of a target in the atmosphere leads to a shift of the center of the emission lines and to an increase in their intensity. Pis’ma Zh. Tekh. Fiz. 23, 31–35 (December 12, 1997)     

Improving the batch distribution at OAO NLMK blast furnace 6

The charging programs for a blast furnace with a nonconical charging system are investigated. On that basis, rational charging conditions are developed and tested. Experimental experience indicates more economical furnace operation, on account of the improved batch distribution over the charge hole and the reduced heat losses through the cooling systems.     

粉末冶金法制备SiCp/Al-Cu-Mg复合材料的固溶时效行为

通过XRD、硬度和电导率测试对比了不同固溶温度下Al-Cu-Mg合金与SiCp/Al-Cu-Mg复合材料中的析出相。XRD分析表明,经T4处理后,未增强基体铝合金中析出相为Al2CuMg相和CuAl2相,而复合材料中的析出相为CuAl2相和Mg2Si相。由SiC颗粒提供的游离Si与基体中的Mg生成了Mg2Si相,从而提高基体合金中Cu/Mg含量比,进而影响Al2CuMg相的生成。