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101.
102.
Kimura T. Sakai Y. Tagashira H. Nakamura S. 《Dielectrics and Electrical Insulation, IEEE Transactions on》1994,1(4):644-647
Fast and slow electron current signals in N2/Ar liquid mixtures have been observed. From the fast signal, the drift velocity and life time of delocalized electrons in the liquid with N2 molar fractions X=N2/(N2+Ar) below 20 mol% were obtained for field strengths E between 5 and 45 kV/cm. The mobility for delocalized electrons in the liquids was shown to decrease exponentially with X as indicated in a previous paper. However, the mobility for localized electrons was shown to increase with increasing X, but only slightly around the N2 liquid value 相似文献
103.
T. Isa T. Fukase M. Sasaki M. Koyano N. Taniguchi T. Kimura Y. Isobe H. Negishi 《Journal of Low Temperature Physics》2002,127(1-2):63-79
We have measured electrical resistivity, Hall coefficient, thermoelectric power, and magnetization for charge-density wave (CDW) material 1T-TaS2 single crystals grown by varying the excess sulfur content x
es. We have revealed that a small mid-gap state is formed inside the Mott gap and that anomalous low temperature transport is not governed by the Mott gap state itself but by the mid-gap state. The electric properties of the mid-gap state are modified by increasing x
es (or hole doping), and we have found the insulator-metal transition occurs by hole doping below 60K. 相似文献
104.
Kimura M 《Neural computation》2002,14(12):2981-2996
This article extends previous mathematical studies on elucidating the redundancy for describing functions by feedforward neural networks (FNNs) to the elucidation of redundancy for describing dynamical systems (DSs) by continuous-time recurrent neural networks (RNNs). In order to approximate a DS on R(n) using an RNN with n visible units, an n-dimensional affine neural dynamical system (A-NDS) can be used as the DS actually produced by the above RNN under an affine map from its visible state-space R(n) to its hidden state-space. Therefore, we consider the problem of clarifying the redundancy for describing A-NDSs by RNNs and affine maps. We clarify to what extent a pair of an RNN and an affine map is uniquely determined by its corresponding A-NDS and also give a nonredundant sufficient search set for the DS approximation problem based on A-NDS. 相似文献
105.
Trehalose was compared with glucose in its use as an embedding medium for the preparation of two-dimensional crystals for electron crystallographic structure analysis. Purple membranes (two-dimensional crystals of bacteriorhodopsin) embedded in either sugar were studied in a 'partially hydrated' state as well as after 'drying' of the specimen. Diffraction patterns of the four conditions, all showing spots to a resolution better than 3.0 A, were analysed and merged. In the case of glucose embedded specimens, the merging R-factor obtained from the diffraction patterns of dried specimens was significantly better than the one obtained from partially hydrated specimens. In the case of trehalose embedding, the merging R-factor for partially hydrated specimens was significantly better than the dried one. The trehalose embedded, partially hydrated specimens gave the best merging R-factor in all four cases. 相似文献
106.
C Mitsuoka K Ohmori N Kimura A Kanamori S Komba H Ishida M Kiso R Kannagi 《Canadian Metallurgical Quarterly》1999,96(4):1597-1602
We provide here evidence that supports the occurrence of a biologically dormant form of selectin ligand carbohydrate, the sialyl 6-sulfo Lewis X containing modified sialic acid, in human leukocytes. The modification of sialic acid involves first de-N-acetylation of sialic acid moiety through ubiquitous de-N-acetylation/re-N-acetylation cycle, followed by the dehydrative cyclization of de-N-acetyl sialic acid to form "cyclic sialic acid." The enzyme involved in the dehydration of de-N-acetyl sialic acid is a calcium-dependent enzyme having neutral-alkaline pH optimum. De-N-acetyl sialyl 6-sulfo Lewis X retained selectin binding activity as well as parental sialyl 6-sulfo Lewis X, but cyclic sialyl 6-sulfo Lewis X was devoid of selectin binding activity. Sialyl 6-sulfo Lewis X carrying the cyclic sialic acid is specifically recognized by the newly generated mAb, G159. The determinant was distributed widely among normal human leukocytes, especially on monocytes and subsets of lymphocytes including NK cells, helper memory T cells, Tcr-gammadelta T cells, and a part of B cells. The determinant was detected also on several cultured lymphocytic leukemia cell lines and O-tetradecanoylphorbol 13-acetate-activated lymphoid cells. Cyclic sialyl 6-sulfo Lewis X is efficiently formed by the action of the partly membrane-bound calcium-dependent enzyme, tentatively called "sialic acid cyclase," and a possible physiological significance of this reaction could be a rapid inactivation of selectin binding activity at the cell surface. Conversely, the accumulated intracellular cyclic sialyl 6-sulfo Lewis X determinant may function as a dormant pool of selectin ligands, which, on appropriate stimulation, is hydrolyzed and becomes active in selectin-dependent cell adhesion. 相似文献
107.
Neutrophil function is reduced in periparturient dairy cows. Possible factors that reduce neutrophil function include endocrine changes associated with parturition and metabolic stresses associated with lactogenesis. In this study, mastectomized and intact cows were studied to specifically examine the effects of lactogenesis on neutrophil function in periparturient cows. Expression of adhesion molecules on neutrophils (L-selectin, mediating capture and rolling adhesion, and beta 2-integrin, mediating tight adhesion vital to egress) and neutrophil myeloperoxidase activity (an index of bactericidal activity) were assessed in mastectomized and intact cows. Expression of L-selectin decreased at parturition followed by rapid recovery to prepartum values in both intact and mastectomized cows. Expression of beta 2-integrins increased in intact cows at parturition but not in mastectomized cows. Expression of beta 2-integrins was greater in intact cows than in mastectomized cows throughout the study. Neutrophil myeloperoxidase activity decreased from baseline prepartum values as parturition approached in both intact and mastectomized cows, which suggests the endocrine changes associated with the act of parturition are predominant factors causing loss of neutrophil function. Myeloperoxidase activity recovered to prepartum values within a week of parturition in mastectomized cows; however, myeloperoxidase activity remained depressed in neutrophils obtained from intact cows throughout the first 20 d of lactation. The presence of the mammary gland and its attendant metabolic stresses slowed recovery of neutrophil function, which suggests that the metabolic stress of lactation exacerbated periparturient immunosuppression. 相似文献
108.
Yamaoka H Hayashi H Karita S Kimura T Sakka K Ohmiya K 《Journal of Bioscience and Bioengineering》1999,88(3):328-330
Chitinase B (ChiB) was purified from the culture supernatant of Xanthomonas sp. strain AK by Phenyl-Toyopearl 650M and DEAE-Toyopearl 650M column chromatographies. The purified enzyme preparation gave a single band on SDS-polyacrylamide gel electrophoresis and the molecular weight of ChiB was estimated to be 48,000. The enzyme was optimally active at pH 6.0 and 60 degrees C. N-Terminal amino acid sequence analysis suggested that ChiB is a member of glycosyl hydrolase family 18 and that it is genetically different from ChiA recently reported (Sakka et al., J. Ferment. Bioeng., 86, 527-533, 1998). Immunological analysis suggested that ChiB was the major chitinase species in the culture supernatant of Xanthomonas sp. strain AK and that production of the enzyme was induced by the presence of chitin. 相似文献
109.
110.
The possible association between the emergence of cytopathogenic HIV-1 variants and disturbance of the cytokine production in the course of HIV-1 infection was studied in 18 infected patients. The cytopathogenicity of the isolates was studied in a microassay based on the use of HIV-1-infectible Hela-CD4 cells carrying the bacterial LacZ gene under the control of the HIV-LTR (P4 cells). In addition, the production of cytokines by heparinized whole blood (HWB) obtained the same day from HIV-1(+) patients was measured. TNF-alpha was determined in a one-step procedure combining HWB culture in the presence of LPS+PHA for 24 h and detection of cytokines in the same wells. In separate experiments HWB was cultured in the presence of LPS+PHA for 48 h, then the supernatants were collected and stored until assayed by ELISa for IFN-gamma and IL-4. Higher TNF-alpha levels were found in activated HWB of patients with cytopathic strains (n = 9) than in patients with non-cytopathic strains (n = 9, p = 0.02) assessed with P4 cells. A defective production of type 1 cytokine (IFN-gamma) and no increased secretion of type 2 cytokines (IL-4) was observed in patients with cytopathic strains. IFN-gamma/IL-4 ratios were significantly lower in patients with cytopathic strains (n = 9) than in other patients (n = 9, p = 0.009). The results show that the disarray of cytokine production, as assessed with whole blood culture, is associated with the cytopathogenicity of HIV-1 isolates in HIV-1-infected individuals. 相似文献