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111.
Fluoroquinolones (FQs) are among the most important antibacterial agents (synthetic antibiotics) used in human and veterinary medicine. An analytical method based on reversed-phase liquid chromatography with fluorescence detection was developed and validated for the simultaneous determination of nine FQs and the quinolone pipemidic acid in urban wastewater. Aqueous samples were extracted using mixed-phase cation-exchange disk cartridges that were subsequently eluted by ammonia solution in methanol. Recoveries were above 80% at an overall precision of better than 10%. Instrumental quantification limits varied between 150 and 450 pg injected. The presented method was successfully applied to quantify FQs in effluents of urban wastewater treatment plants. The two most abundant human-use FQs, ciprofloxacin and norfloxacin, occurred in primary and tertiary waste-water effluents at concentrations between 249 and 405 ng/L and from 45 to 120 ng/L, respectively. The identity of FQs in urban wastewater was confirmed by recording full fluorescence spectra and liquid chromatography directly coupled to tandem mass spectrometry. These results indicate that conventional environmental risk assessment overestimates FQ concentrations in surface waters by 1 to 2 orders of magnitude. 相似文献
112.
Leticia Hosta‐Rigau Brigitte Städler Yan Yan Edouard Collins Nice Joan K. Heath Fernando Albericio Frank Caruso 《Advanced functional materials》2010,20(1):59-66
Therapeutic artificial cells or organelles are nanoengineered vehicles that are expected to substitute for missing or lost cellular function. The creation of capsosomes, polymer carrier capsules containing liposomal subcompartments, is a promising approach towards constructing such therapeutic devices using the layer‐by‐layer assembly method. Herein, the assembly of intact, nonaggregated capsosomes containing multiple liposome layers is reported. It is also further demonstrated that thiocoraline, a hydrophobic model peptide with antitumor activity, can be efficiently loaded into the membrane of the liposomal subcompartments of the capsosomes. Cell viability assays verify the activity of the trapped antitumor cargo. It is also shown that pristine capsosomes do not display inherent cytotoxic effects. The ability to tune the number of liposome layers and hence the drug loading in capsosomes as well as their noncytotoxicity provide new opportunities for the creation of therapeutic artificial cells and organelles. 相似文献
113.
Stephan Krenek Klaus Anhalt André Lindemann Christian Monte Jörg Hollandt Jürgen Hartmann 《International Journal of Thermophysics》2010,31(4-5):998-1010
The laser-flash method is a fast, widely used and well established technique to measure the thermal diffusivity. Since its introduction in the 1960s, it was proposed to expand this technique to the measurement of heat capacity and emissivity. Currently, the measurement of spectral emissivity at high temperatures is connected with relatively large uncertainties, although the spectral emissivity is an essential parameter for applications, e.g., in the lamp industry and fusion research. In this work, a theoretical study is presented on the possibility of emissivity measurements using the laser-flash method. Two mathematical approaches are discussed which solve the problem, that a measured temperature rise—necessary to calculate the emissivity—itself depends on the emissivity. It is shown that both methods have a negligible arithmetic error, making them applicable to be used in future work. 相似文献
114.
A new approach to estimate vehicle tire forces and road maximum adherence is presented. Contrarily to most of the previous works on this subject, it is not an asymptotic observer-based estimation, but a combination of elementary diagnosis tools and new algebraic techniques for filtering and estimating derivatives of noisy signals. In a first step, instantaneous friction and lateral forces will be computed within this framework. Then, extended braking stiffness concept is exploited to detect which braking efforts allow to distinguish a road type from another. A weighted Dugoff model is used during these ‘distinguishable’ intervals to estimate the maximum friction coefficient. Very promising results have been obtained in noisy simulations and real experimentations for most of the driving situations. 相似文献
115.
Canonical processes of semantically annotated media production 总被引:1,自引:1,他引:0
Lynda Hardman Željko Obrenović Frank Nack Brigitte Kerhervé Kurt Piersol 《Multimedia Systems》2008,14(6):327-340
116.
What makes virtual violence enjoyable rather than aversive? Two 2×2 experiments tested the assumption that moral disengagement cues provided by a violent video game's narrative and game play lessen users' guilt and negative affect, which would otherwise undermine players' enjoyment of the game. Experiment 1 found that users' familiarity with the violent game reduced guilt and negative affect, and enhanced enjoyment, whereas opponents' nonhuman outer appearance and blameworthiness had no effect. Experiment 2 found that fighting for a just purpose, perceiving less mayhem, and framing the overall situation as “just a game” or “just an experiment” reduced guilt and negative affect, whereas the distorted portrayal of consequences did not. Effects on game enjoyment were mixed and suggest that moral disengagement cues may both foster and diminish game enjoyment. 相似文献
117.
Algorithms for the maximum satisfiability problem 总被引:2,自引:0,他引:2
Old and new algorithms for the Maximum Satisfiability problem are studied. We first summarize the different heuristics previously proposed, i.e., the approximation algorithms of Johnson and of Lieberherr for the general Maximum Satisfiability problem, and the heuristics of Lieberherr and Specker, Poljak and Turzik for the Maximum 2-Satisfiability problem. We then consider two recent local search algorithmic schemes, the Simulated Annealing method of Kirkpatrick, Gelatt and Vecchi and the Steepest Ascent Mildest Descent method, and adapt them to the Maximum Satisfiability problem. The resulting algorithms, which avoid being blocked as soon as a local optimum has been found, are shown empirically to be more efficient than the heuristics previously proposed in the literature. 相似文献
118.
The initiating ability of tetramethylene zwitterions formed from cyclobutane adducts of donor olefins with TCNE was investigated. Polar solvents increased the ability of vinyl ether-TCNE cyclobutane adducts to initiate the cationic polymerization of N-vinylcarbazole. The concept of charge separation in the tetramethylene zwitterions was also investigated. 相似文献
119.
Axel Kramer Prof. Dr. Ojan Assadian Prof. Dr. H. Below Dr. rer. nat. Claudia Bender Aylin Hammann Bernd Hartmann Dr. Nils‐Olaf Hübner Dr. med. Thomas Kocher Prof. Dr. Jürgen Lademann Prof. Dr. Dr.‐Ing. Rutger Matthes Dipl. human. biol. Ina Koban Dipl. human. biol. Klaus‐Dieter Weltmann Prof. Dr. 《真空研究与实践》2010,22(2):33-38
Prospects of plasma medicine: Applications of Tissue Tolerable Plasmas (TTP) Based on the current knowledge on the physical properties and biological effects of tissue tolerable plasma (ttp) potential perspectives of plasma medicine are discussed. Currently, the foundations for the following medical applications of ttp are developed by an interdisciplinary research team: Prevention and/or treatment of diseases such as chronic wounds, skin and mucosal infectious diseases, localized tumors, promotion of angiogenesis, and tissue ablation Inhibition and/or elimination of biofilms by prevention of biofilm development due to surface treatment and/or plasma steered application of antimicrobial active layers with drug delivery function on foreign objects implanted into humans (implantable prosthesis, contact lenses, stents etc.) as well as elimination of biofilms by direct action of ttp on surfaces and tissues (chronic wounds, tooth surface, prosthesis) promotion of the incorporation of implants into viable tissue by changing the surface of materials (hydrophobicity) Promotion of penetration of topically applied drugs with therapeutic results Assessment of veterinary indications Improved cleaning performance in reprocessing of medical devices by surface modification. 相似文献
120.