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51.
CLAUDIA RUIZ-CAPILLAS CAROL M. GILLYON WILLIAM F.A. HORNER 《Journal of Food Biochemistry》2001,25(6):541-553
Flesh concentration of Trimethylamine Oxide‐Nitrogen (TMAO‐N), Trimethylamine‐Nitrogen (TMA‐N) and Total Volatile Base Nitrogen (TVB‐N) are the most studied parameters used as indices of spoilage in fish and fishery products. In the present work, the official methods to determine TVB‐N, TMA‐N and TMAO‐N were compared with the Flow Injection Analysis (FIA) technique using three different fish species over their storage in melting ice for a period of 16 days. There was a good correlation between determinations made using official and FIA methods with no apparent significant differences. FIA thus appears to be a suitable method for TMAO‐N, TMA‐N and TVB‐N in fish extract and the use of this system is much faster and simpler than the official methods for determination of these parameters. On the other hand, the individual concentrations of TMAO‐N, TMA‐N and TVB‐N were not found to represent reliable indices of spoilage in fish, which had been frozen and thawed and then presented in chilled format. 相似文献
52.
CLAUDIA L. NATALUCCI ADRIANA BRULLO LAURA M.I. LÓPEZ ROSANA M. HILAL NÉSTOR O. CAFFINI 《Journal of Food Biochemistry》1995,19(6):443-454
A cysteine proteinase was isolated from immature fruits of Pseudananas macrodontes (Morr.) Harms, a species taxonomically close to pineapple grown in the northeast of Argentina. The protease is active at pH 7.0 to 10.5, and rather unstable at temperatures over 45C. Crude extracts were purified by acetone fractionation followed by chromatofocusing at the pH range 4.5 to 6.5. Two main proteolytic fractions were obtained: fraction I (Mr= 20.6 kDa, pI = 5.9) and fraction II (Mr= 19.3 kDa, pI = 5.3), both homogeneous by gel-filtration and SDS-PAGE criteria. This new plant protease (“macrodontin”) could be valuable in processes carried out in neutral-weak alkaline media. The thermal behavior of the crude enzyme is also a useful property, since it could be easily inactivated in foodstuffs by heating 10 min at 75C. Comparison of macrodontin and alcalase proteolytic behavior on soy concentrates is presented. 相似文献
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This work studied the development of free amino acids (FAAs) and dipeptide anserine as quality indices for gutted hake stored in ice for 25 days. The correlation of these compounds was determined with total volatile basic nitrogen (TVBN) which has been used as a quality index, for fish stored in ice. The most abundant free amino acids in hake muscle were found to be threonine, glycine, alanine, glutamic acid, β-alanine methylhistidine. lysine and the dipeptide, anserine. The only hydrophobic free ammo adds which exhibit significant differences (P<0.05) throughout storage was tryptophan. moreover, this amino acid exhibited a very high correlation (r=0.951) with TVBN. A significant decrease in anserine (P<0.05) correlated with the increases in 1-methylhistidine and β-alanine throughout storage. These changes also exhibited a very high correlation with TVBN. Therefore, 1-methylhistidine, β-alanine anserine and tryptophan could be used as quality parameters for hake stored in ice. 相似文献
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CLAUDIA P. OSTERTAG 《Particulate Science and Technology》2013,31(3-4):133-142
ABSTRACT The paper discusses the processing and the resulting mechanical properties of sintered fiber reinforced ceramic matrix composites. In situ observations of the sintering process revealed that stresses which develop due to the differential shrinkage between the fibers and the matrix initiate already during the heating cycle and are of sufficient magnitude and duration that crack like damage forms. Successful methods were employed for reducing and avoiding these stresses during the sintering process. Coarse grained alumina coatings deposited onto the fibers with a coating thickness of up to 10 µm delayed and reduced the stress development. Polymer coated fibers produced fully dense composites on which fracture toughness measurements were performed. Crack propagation and crack/fiber interaction was observed in situ inside a scanning electron microscope. The importance of studying both the crack front and the crack wake phenomena in fiber reinforced composites is illustrated. In specimens where the cracks are already bridged by 10% area fraction of fibers a toughness of 7 MPa √m was obtained. However, in samples where the cracks are not bridged yet by fibers, the crack becomes unstable before reaching the fiber positions and the fibers had no effect in resisting the crack propagation. 相似文献
57.
HUGO A. ROLDÁN SARA I. ROURA CLAUDIA L. MONTECCHIA OLINDA PÉREZ BORLA MARCOS CRUPKIN 《Journal of Food Biochemistry》2005,29(2):187-204
The lipid composition of frozen stored fillets from pre‐ and postspawned hake was studied. The total lipid (TL) content in the chloroform/methanol extract from unfrozen postspawned hake was four times higher than that of prespawned fish. After freezing, the TL content of postspawning hake muscle remained unchanged whereas the TL extracted from prespawning fish muscle increased about 90%. The TL extractability of muscle from fish in both different gonadal conditions was not affected by frozen storage. Lipolysis in frozen stored fillets from prespawned hake occurs principally by hydrolytic action on phospholipids (PL), and phosphatidylcholine was the main PL hydrolyzed. Triacylglycerols were the main substrates hydrolyzed in frozen stored fillets from postspawned hake. Freezing and frozen storage affected polyenoics and n‐3 fatty acids (FA). The decrease in the contents of n‐3 FA in fillets from postspawned hake was lower than that observed in fillets from prespawned fish. 相似文献
58.